FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005284314

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284314
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5600394
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA238542442.59386036053892No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA1950653.483058513383166No Hit
TCTTAACCCGGACCAGAAACTATGGAATTCTCGGGTGCCAAGGAACTCCA1033591.8455665797799226RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA912581.6294924964207875RNA PCR Primer, Index 1 (100% over 28bp)
AGGTTCCGGATAAGTAAGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCA809711.4458089912959695RNA PCR Primer, Index 1 (100% over 28bp)
GTCCCACTCCGTAGATCTGTTCTGGAATTCTCGGGTGCCAAGGAACTCCA743811.3281386988129762RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGAATTCTCGGGTGCC670191.196683661899502No Hit
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG434970.7766774980474589RNA PCR Primer, Index 1 (100% over 29bp)
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA418400.7470902940043147RNA PCR Primer, Index 1 (100% over 23bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC372490.665113918770715RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA369740.6602035499645204RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGC305150.5448723786219326RNA PCR Primer, Index 12 (100% over 50bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC272240.48610865592670804RNA PCR Primer, Index 1 (100% over 26bp)
GCTCTACACTCTACTCGTCAGCTGTTGGAATTCTCGGGTGCCAAGGAACT261020.4660743511974336RNA PCR Primer, Index 1 (100% over 25bp)
ATTCCCGTGTGAAAGTAGGTAATCGTCAGGCTTGGAATTCTCGGGTGCCA259450.46327097700626063No Hit
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC231000.4124709797203554RNA PCR Primer, Index 1 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC195890.349778961980175RNA PCR Primer, Index 1 (100% over 27bp)
TGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA183000.3267627241940478No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACATGGAATTCTCGGGTGCCA181480.3240486294357147No Hit
CCCGTGTGAAAGTAGGTAATCGTCAGGCTTGGAATTCTCGGGTGCCAAGG175890.3140671888442135Illumina Small RNA Adapter 2 (100% over 21bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA152940.27308792917069763RNA PCR Primer, Index 1 (100% over 28bp)
TATGCATATGATCACGAGACTCAGTTGGAATTCTCGGGTGCCAAGGAACT148150.26453495950463485RNA PCR Primer, Index 1 (100% over 25bp)
CGATGCTATAGACTCTCACGTGATGTGGAATTCTCGGGTGCCAAGGAACT146800.26212441481795745RNA PCR Primer, Index 1 (100% over 25bp)
GATTCCCGTGTGAAAGTAGGTAATCGTCAGGCTTGGAATTCTCGGGTGCC144500.25801756090732186No Hit
CGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA143380.256017701611708No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA132550.2366797764585849RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA132430.23646550581976913RNA PCR Primer, Index 1 (100% over 28bp)
AGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA124790.2228236084818318No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC124200.22177011117432097RNA PCR Primer, Index 1 (100% over 26bp)
CCGTGTGAAAGTAGGTAATCGTCAGGCTTGGAATTCTCGGGTGCCAAGGA122340.2184489162726765RNA PCR Primer, Index 1 (100% over 22bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC114840.20505700134669097RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTGCCAAGGAACTCC114620.2046641718421954RNA PCR Primer, Index 1 (100% over 27bp)
CCCCCCACTGCTAAATTTGACTGGCTTTTGGAATTCTCGGGTGCCAAGGA108470.19368280160288723RNA PCR Primer, Index 1 (100% over 22bp)
TAGCATATCGAGCCTGAGAACATGGAATTCTCGGGTGCCAAGGAACTCCA93490.16693468352405205RNA PCR Primer, Index 1 (100% over 28bp)
TCCCGTGTGAAAGTAGGTAATCGTCAGGCTTGGAATTCTCGGGTGCCAAG92990.16604188919565302No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA90670.1618993235118815RNA PCR Primer, Index 1 (100% over 28bp)
GGATTCCCGTGTGAAAGTAGGTAATCGTCAGGCTTGGAATTCTCGGGTGC88920.15877454336248484No Hit
CGGATTCCCGTGTGAAAGTAGGTAATCGTCAGGCTTGGAATTCTCGGGTG85600.15284638902191525No Hit
GGCAGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA84100.15016800603671812No Hit
TGATACGGATGTTATACGCAGCTGGAATTCTCGGGTGCCAAGGAACTCCA83540.14916807638891122RNA PCR Primer, Index 1 (100% over 28bp)
CCTGGAACTTAGGACGTGAATCTGGAATTCTCGGGTGCCAAGGAACTCCA80290.14336491325431747RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTTGGAATTCTCGGGT77270.13797243551078728No Hit
TGAGGTAGTAGATTGTATAGTTTTGGAATTCTCGGGTGCCAAGGAACTCC76990.1374724706868838RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA70230.1254018913669288RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA70190.1253304678206569RNA PCR Primer, Index 1 (100% over 28bp)
TTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCC69330.12379486157581056No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC68990.12318776143249921RNA PCR Primer, Index 1 (100% over 27bp)
GGATGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA68820.12288421136084353No Hit
CGTGTGAAAGTAGGTAATCGTCAGGCTTGGAATTCTCGGGTGCCAAGGAA68690.1226520848354598RNA PCR Primer, Index 1 (100% over 23bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC60420.10788526664373972RNA PCR Primer, Index 1 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)