FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005284317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51470855
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGC1075230.2089007458687057No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGC765200.14866665805337798No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT623730.12118120050657795No Hit
CCCAGATTTATTGAAAATAATACAGCACTACAGAAAAAATTCAAACAGGT561440.10907920608662902No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGC546000.10607945020536379No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC531730.10330700743168147No Hit
GTCATGTCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG527410.10246769749599069No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)