FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005284319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7078398
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA445870662.99032634220342No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA3307944.67328906908032No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGAATTCTCGGGTGCC1468542.074678479509064No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACATGGAATTCTCGGGTGCCA500740.7074199557583509No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTTGGAATTCTCGGGT393950.5565524854635187No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG310490.4386444503403171No Hit
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA285700.40362240156600404RNA PCR Primer, Index 1 (100% over 28bp)
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA248510.3510822646593198RNA PCR Primer, Index 1 (100% over 23bp)
TTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCC222740.31467572182293224No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTATGGAATTCTCGGG181680.25666824612009664No Hit
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGC178110.2516247320368253No Hit
TCTTAACCCGGACCAGAAACTATGGAATTCTCGGGTGCCAAGGAACTCCA166020.23454459610776335RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATGGAATTCTCGGGTGC146120.20643089015339347No Hit
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC134190.18957679407120084RNA PCR Primer, Index 1 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTATCGGGTGCCA132000.18648287366717725No Hit
TGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA131610.18593190154043332No Hit
GGGTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA115040.16252264989903084No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA112340.1587082274831113RNA PCR Primer, Index 1 (100% over 28bp)
TCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCCAAGG110480.15608051426325562Illumina Small RNA Adapter 2 (100% over 21bp)
AGGTTCCGGATAAGTAAGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCA97610.13789843408070585RNA PCR Primer, Index 1 (100% over 28bp)
GGATGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA95730.13524246587999147No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGTATGC94100.13293968494001043RNA PCR Primer, Index 13 (100% over 50bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC90810.12829174058876033RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG89450.12637040189037124RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC88580.1251413102230194RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA88020.12435017075897682RNA PCR Primer, Index 1 (100% over 28bp)
TAGGATGGGTGTGATAGGTGGCACGGAGAATTTTGTGGAATTCTCGGGTG87760.1239828560078142No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAACTGGAATTCTCG86380.12203326232856644No Hit
AGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA85720.12110084796023056No Hit
TAGGATGGGTGTGATAGGTGGCACGGAGAATTTGGAATTCTCGGGTGCCA85530.12083242564207325No Hit
TAGGATGGGGTGTGATAGGTGGCACGGAGAATTTGGAATTCTCGGGTGCC84160.11889695945325482No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAACTTGGAATTCTC83420.11785152516148428No Hit
CGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA77760.10985536557848259No Hit
ATTCCCGTGTGAAAGTAGGTAATCGTCAGGCTTGGAATTCTCGGGTGCCA73270.1035121223757127No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTTGGAATTCTCGGGTGCCAAGGA73140.10332846500013138RNA PCR Primer, Index 1 (100% over 22bp)
CGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCCAAGGA72720.10273511040209947RNA PCR Primer, Index 1 (100% over 22bp)
CATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTG71040.10036169200997175No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)