FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005284331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4436759
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA93985821.18343592699085RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC3565668.03663214522132RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG1095932.470113882678775RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC705011.5890202735825858RNA PCR Primer, Index 1 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA695671.5679688709709045No Hit
CTTATCGGATTGGAGGGGCTGCTGAGCGGTTCCTGGAATTCTCGGGTGCC359650.8106142343994794No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA277560.6255917889612665RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC133890.30177433572569523RNA PCR Primer, Index 1 (100% over 27bp)
CCTTATCGGATTGGAGGGGCTGCTGAGCGGTTCCTGGAATTCTCGGGTGC98170.22126511717224218No Hit
TTCGACCTGAGCTCAGGCAAGGCTACCCGTGGAATTCTCGGGTGCCAAGG97710.2202283243241294Illumina Small RNA Adapter 2 (100% over 21bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA90490.20395518440375057RNA PCR Primer, Index 1 (100% over 28bp)
AGATGGTGACGGATCGCAGGTGTAGTTCCTGGAATTCTCGGGTGCCAAGG89810.2024225341065404Illumina Small RNA Adapter 2 (100% over 21bp)
GCAGGCCTATGCTCTGAATACATTAGCATGGAATTCTCGGGTGCCAAGGA89750.20228730025678654RNA PCR Primer, Index 1 (100% over 22bp)
CAACTCTTAACAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGT80010.18033433864674642RNA PCR Primer, Index 16 (100% over 37bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTATGC77990.17578146570503378RNA PCR Primer, Index 16 (100% over 50bp)
CAGGCCTATGCTCTGAATACATTAGCATGGAATTCTCGGGTGCCAAGGAA69140.1558344728663423RNA PCR Primer, Index 1 (100% over 23bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA68570.15454975129368081No Hit
CAAACGAGAACTTTGAAGGCCGAAGTGGAATTCTCGGGTGCCAAGGAACT66440.14974894962741947RNA PCR Primer, Index 1 (100% over 25bp)
GCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA65770.14823883830516824RNA PCR Primer, Index 1 (100% over 28bp)
TTCGACCTGAGCTCAGGCAAGGCTACCCTGGAATTCTCGGGTGCCAAGGA62140.1400571903950609RNA PCR Primer, Index 1 (100% over 22bp)
TCCTGTACTGAGCTGCCCCGAGTGAATTCTCGGGTGCCAAGGAACTCCAG58130.13101906143651254RNA PCR Primer, Index 1 (100% over 27bp)
CTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGT56260.12680427311918452RNA PCR Primer, Index 1 (100% over 30bp)
AAGCAGGCCTATGCTCTGAATACATTAGCATGGAATTCTCGGGTGCCAAG55990.12619572079529223No Hit
GATGGTGACGGATCGCAGGTGTAGTTCCTGGAATTCTCGGGTGCCAAGGA54460.1227472576265693RNA PCR Primer, Index 1 (100% over 22bp)
CTTACTGAGTCTAAGCAAACTCCGAATACCGAGAATGGAATTCTCGGGTG54250.12227393915243087No Hit
AGCAGGCCTATGCTCTGAATACATTAGCATGGAATTCTCGGGTGCCAAGG54050.12182315965325138Illumina Small RNA Adapter 2 (100% over 21bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC54040.1218006206782924RNA PCR Primer, Index 2 (100% over 34bp)
TCTTAACCCGGACCAGAAACTATGGAATTCTCGGGTGCCAAGGAACTCCA54010.1217330037534155RNA PCR Primer, Index 1 (100% over 28bp)
TTTGAACGCAAATTGCGCCCGAGGTGGAATTCTCGGGTGCCAAGGAACTC53280.12008765858141045RNA PCR Primer, Index 1 (100% over 26bp)
TCCTGTACTGAGCTGCCCCGAGATTGGAATTCTCGGGTGCCAAGGAACTC50860.11463322664133886RNA PCR Primer, Index 1 (100% over 26bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA50660.11418244714215942RNA PCR Primer, Index 1 (100% over 32bp)
GGCTTTATAGTGTAAGGGCTATCACATAGGATTCTGATTCCTGGAATTCT50580.11400213534248761No Hit
AAACGAGAACTTTGAAGGCCGAAGTGGAATTCTCGGGTGCCAAGGAACTC50390.11357389481826712RNA PCR Primer, Index 1 (100% over 26bp)
TCCTGTACTGAGCTGCCCCGAGAATGGAATTCTCGGGTGCCAAGGAACTC48340.10895340495167757RNA PCR Primer, Index 1 (100% over 26bp)
CTTACTGAGTCTAAGCAAACTCCGAATACCGAGATGGAATTCTCGGGTGC48080.10836739160274425No Hit
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG47690.1074883715793443Illumina Small RNA Adapter 2 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)