FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005284334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29381037
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCA2416160.8223535472897026No Hit
GTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGT2361190.8036442008496841No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA2227250.758056973959088No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACAC1987660.6765111796428424No Hit
CAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAG1749920.5955950431565775No Hit
CACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCA1649990.5615833096701114No Hit
GGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACC1268710.4318125326890266No Hit
CCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGAGGGTTC1267380.43135985976260804No Hit
CCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACT1044740.35558309259132004No Hit
AATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATG1001870.3409920487149586No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT986180.33565186960555543No Hit
CCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTG903970.30767123706355226No Hit
TGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAAT850680.289533688004273No Hit
CCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTC762900.25965727486065243No Hit
CTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTT751730.25585550298990467No Hit
GCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGG740370.25198906355823997No Hit
GTTTACCAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCT682940.23244244238213918No Hit
CGCATTTTGAAAATTCTATGGAAGAGCTAGCATCTCTGACGAAAACAGCA675670.2299680572881073No Hit
CGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGG659060.22431475104163273No Hit
CTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAAAAATCCACTGCCTTCCAA643850.21913794261244082No Hit
CACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCA643820.21912773194492763No Hit
TGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAA642130.21855253100835073No Hit
TATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAA621490.21152759175926977No Hit
GGGGAAACGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCATTCA611450.2081104216981858No Hit
CTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACT610950.20794024390629914No Hit
ATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGC595350.20263069679943563No Hit
GGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGT591470.20131011713439523No Hit
TGAAAATTCTATGGAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCACACAAAA558870.19021452510338557No Hit
CCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCAT555530.18907773745358272No Hit
CTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCAC516970.1759536261432842No Hit
CGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTA502860.1711512088562429No Hit
CACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGC501820.17079723904911864No Hit
CTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGC494540.168319450399249No Hit
CATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATG491740.16736645476468376No Hit
CGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCATTCATGAAATC466970.15893584695461907No Hit
ACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTT465350.1583844709089063No Hit
CTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACT458630.15609728138594972No Hit
CAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATA444100.15115191475372364No Hit
GTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATA443350.15089664806589365No Hit
CAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCA439270.14950799728409858No Hit
CGAATAGCTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAG437650.14895662123838585No Hit
TGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTG427600.14553604762146413No Hit
CCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTAT413890.14086977256793218No Hit
TGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGAC411590.14008695472525357No Hit
GTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAG410280.13964108891051052No Hit
ACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGAGGGTTC405620.13805503189012694No Hit
CTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGG385780.13130237710806464No Hit
CGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGGTCAGTAATTCCTGGAGGAATAGGTACCAAGAAAAA373170.12701049319668328No Hit
GGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCG372090.12664290916620813No Hit
CTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAAGACGAGAAGACCCTATGG356700.121404836731937No Hit
AGATGATTGACCGCACGCAATTGATATTAGATATTTTTGCAAAGCGGGCG354020.12049268376742456No Hit
CATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCA352370.1199310970541986No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT340290.1158196016022171No Hit
GAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATT336630.11457390016560681No Hit
CAATAACGTCTCTTTCAGAAGGCATTGGTATCTTTTCCCCACTTCCAAGC336140.11440712592955789No Hit
AAAACGAATAGCTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCC331610.11286531513506484No Hit
GCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACAC330500.11248752043707647No Hit
GTTTTACTAGGCTAGACTAGCGTACGAGCACTATGGTCAGTAATTCCTGGAGGAATAGGTACCAAGAAAAAAACG330010.11232074620102755No Hit
CCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAG329500.11214716485330317No Hit
AGAACAACCGCCGCATACGGCCGATTGTCGCAGCCCGGGTCGATTATAAC328600.11184084482790721No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA324800.11054749360956864No Hit
CAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAA322640.1098123255486183No Hit
CTATGGAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCACACAAAAACGGAAC312700.1064291910459117No Hit
ATTCTATGGAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCACACAAAAACGGA309400.10530601761945979No Hit
GGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA307600.10469337756866784No Hit
CCTTTCCCGGCAAGGCGGAGGAATTGGGGCAAGAGGTCCCGGGGAAACGA303750.10338300857114065No Hit
GGCAAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACC299350.1018854440025381No Hit
GGCCGATTGTCGCAGCCCGGGTCGATTATAACAACGGTGCAATCTCAGCTAAACCGACGCAGTTTTGCTCCTTGG297760.10134427862433855No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT296590.10094606259132378No Hit
CAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTC294220.10013941985778105No Hit
ACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCA294000.10006454162935093No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTCGG19200.042.855921
CGCGAAC9250.042.82072452
TACGTCA12200.042.1248827
ACTTTCG21450.038.68332320
ATGCGTA2350.038.4092378
CGGATTA21550.038.02575725
ACGCCAC61800.037.2939762
CGGTACG2700.037.2917323
CGTATAA8950.037.2528833
CGCATTT139300.037.0789341
TTCGCGA19150.036.5798369
CGTCGAA6000.036.5247663
ATAGCGG5500.035.9905330
CGCTAAC6100.035.4270
ATTACGT5400.034.75010755
TATAGCG9400.034.71533217
TCGGATT23950.034.069124
TCGCGAA12050.033.44593451
GTAGCGA5450.032.50203339
CGGTTAT3750.032.3975521
Produced by FastQC (version 0.11.5)