FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005284335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2333122
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA698972.995857053338831No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC267371.1459752211843188No Hit
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC209840.899395745271786RNA PCR Primer, Index 2 (100% over 34bp)
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA150060.6431725387699401RNA PCR Primer, Index 1 (100% over 23bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA147870.633785974329675RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC88930.381163093914506RNA PCR Primer, Index 1 (100% over 27bp)
TCTTAACCCGGACCAGAAACTATGGAATTCTCGGGTGCCAAGGAACTCCA85680.36723326084105334RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA80650.34567416534583273No Hit
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG70760.3032846117776953RNA PCR Primer, Index 1 (100% over 29bp)
GCTCTACACTCTACTCGTCAGCTGTTGGAATTCTCGGGTGCCAAGGAACT43450.18623115293585163RNA PCR Primer, Index 1 (100% over 25bp)
AGGTTCCGGATAAGTAAGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCA43140.18490246116576844RNA PCR Primer, Index 1 (100% over 28bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGT40330.17285851318533707RNA PCR Primer, Index 12 (97% over 36bp)
ATCTTCTGTAGACGACTTAACATGGAATTCTCGGGTGCCAAGGAACTCCA37690.1615432026272094RNA PCR Primer, Index 1 (100% over 28bp)
CCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAAC37170.15931442933545695RNA PCR Primer, Index 1 (100% over 24bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG36030.15442827250353816RNA PCR Primer, Index 2 (100% over 35bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG33330.14285579579636212Illumina Small RNA Adapter 2 (100% over 21bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCGTATGC30660.13141190216371026RNA PCR Primer, Index 22 (100% over 50bp)
GTCCCACTCCGTAGATCTGTTCTGGAATTCTCGGGTGCCAAGGAACTCCA27810.11919651008391331RNA PCR Primer, Index 1 (100% over 28bp)
CTGTCGTCCAAACTAAGCCTAAAGCACCGTGGAATTCTCGGGTGCCAAGG25750.11036713896658641Illumina Small RNA Adapter 2 (100% over 21bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC23450.10050910325306607No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)