FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005284342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51875184
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTC8041231.550111128280528No Hit
GTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGG7465491.4391254978488366No Hit
CGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATT5638461.0869281928715664No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG4061660.7829678252321958No Hit
CTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACT3963900.7641225908711958No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT3787790.7301737956245129No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGC3183370.613659510104099No Hit
GTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGT2972390.5729888109890848No Hit
GTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCC2880570.5552886328075483No Hit
GGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCG2870920.5534283984419216No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTC2703670.5211875489444048No Hit
GGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTT2479720.4780166177338282No Hit
GGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCT2142260.4129643183530684No Hit
GGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGAC2037130.3926983661397712No Hit
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT1969770.3797133519564962No Hit
GTCAGACAAAGGATAAACGGTGAAAATTAACAAGAAGCAATACAGAGTATGGTTTAGGGCCCTGGATCGTGCG1702750.32823979959280725No Hit
GCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCG1601170.3086581823015799No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTCTAATACTGGTG1600160.308463484196991No Hit
GGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTG1525370.29404618593738385No Hit
CACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACA1470310.2834322476812805No Hit
GTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTC1346170.2595017301528993No Hit
AGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGAC1280020.2467499681543298No Hit
CTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA1262410.2433552814000621No Hit
CCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA1259300.2427557654542488No Hit
GTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTT1257800.2424666098533742No Hit
GGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATT1222960.23575048909706037No Hit
GCGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGAT1220110.2352010934553986No Hit
GTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATT1210810.23340832872997616No Hit
GCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG1157080.22305077510664828No Hit
GCTAATTTATATACGTCACGGCTTCTATTCGATGATCCGATATGGTAAAATTTATTCAGACTGTAAAGTAGGACC1060650.20446192537842373No Hit
GGGACTCTTTTGCCAACTTGGGAATGTTCGCGAATATATGCTGAAGCCGT1046010.20163976671388772No Hit
CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAATGTGTCCTGC990790.19099498519369107No Hit
GGCCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGGGAATGTTCGCGAATATATGCTGA953230.1837545289477913No Hit
CTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGG950770.18328031376235698No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT907780.17499311424129116TruSeq Adapter, Index 8 (97% over 36bp)
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTC895220.17257191800996793No Hit
AGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATT886440.17087939389284865No Hit
CTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGG862470.16625868739087268No Hit
GTCACGGCTTCTATTCGATGATCCGATATGGTAAAATTTATTCAGACTGT861100.1659945919420739No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTT854460.16471459648220235No Hit
CGACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCG836920.16133340365597548No Hit
GGTTTAGGGCCCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTT834330.16083412831846533No Hit
GTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCA794690.1531927096393528No Hit
GTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG791290.1525372902773704No Hit
CTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATG790680.15241970033301472No Hit
GGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTC786370.15158885990650173No Hit
GCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGT760680.1466365883155229No Hit
GTAGAGAGTATGTTAATTGGGAAATGAAGCAATAATGATGTGGCTTATGCTTACTGGTAAAAAGACTTTGTAATG747800.14415370555601306No Hit
ATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG718650.13853444837901682No Hit
CAACTTGGGAATGTTCGCGAATATATGCTGAAGCCGTGTCTCTGTGGACGGGAAAGGGCGAATCATAGGGAGGTG717440.13830119619431133No Hit
GGACTCTTTTGCCAACTTGGGAATGTTCGCGAATATATGCTGAAGCCGTG712570.13736240434347183No Hit
GGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATT710380.13694023716619494No Hit
GAATGTTCGCGAATATATGCTGAAGCCGTGTCTCTGTGGACGGGAAAGGG710070.13688047834201417No Hit
CTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTA703480.1356101214021718No Hit
CAAAGGATAAACGGTGAAAATTAACAAGAAGCAATACAGAGTATGGTTTAGGGCCCTGGATCGTGCGAGAGAA691160.13323519006698847No Hit
GCTGAAGCCGTGTCTCTGTGGACGGGAAAGGGCGAATCATAGGGAGGTGA687860.1325990477450644No Hit
CATCTATCCAGTGAGTCCTGCAAGACTTCAGGCTCTACTACCTCCAGCAG685880.13221736235190992No Hit
CACTCTGAGCTGCCTGGATATCCAGGGCGATGTCCAGGTAAGGGTCAAAAGTGTCTGAAATGCCGTGGCAGTGG685730.13218844679182248No Hit
AGGAATGAGAATTAAACAGTGGAAGGAATAAAACACTGTAGAGAAGCTATTCGATGTCCTACATATAGTTATAGA684220.13189736348694206No Hit
CTTGGGAATGTTCGCGAATATATGCTGAAGCCGTGTCTCTGTGGACGGGA677030.13051134430674985No Hit
GCAGGTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATT675760.13026652589800933No Hit
CACGGCTTCTATTCGATGATCCGATATGGTAAAATTTATTCAGACTGTAAAGTAGGACCATAGGGGAATTAAGGA674680.13005833386537963No Hit
CGGGCCCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAA673930.12991375606494235No Hit
AGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTT671020.12935279419924564No Hit
ACGGCTTCTATTCGATGATCCGATATGGTAAAATTTATTCAGACTGTAAAGTAGGACCATAGGGGAATTAAGGA670300.12921399951082582No Hit
CTTCCGTACGCCACATGTCTCGCGCCCCGCCGCGGGGCGGGGATTCGGCG656790.1266096713989487No Hit
GTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGTTTCGTTTCCCCGGGACCTCTTGCCCC654800.12622605830178837No Hit
GACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGT649150.1251369055384941No Hit
CTAGGCTAATTTATATACGTCACGGCTTCTATTCGATGATCCGATATGGT639270.12323233398073344No Hit
AAGGGGAGTGGTGTCTAAATTGACCCTGAAAACTTACAGTGCGTCTAGGT636600.12271763701117668No Hit
GGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAAT625140.12050848822049479No Hit
GCCTACAGCACCCGGTATTCCCAGGCGGTCTCCCATCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCG622050.11991282768269314No Hit
CGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTC619400.11940198612114804No Hit
CTGCGGATGTATTTTTCACCCAAGAAATTTCGGATTTCAACAAGAGACCC618210.1191725893444542No Hit
GCTGGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGTTGAATAACAT607570.11712151228225041No Hit
GGGAGCTAGCCCCCCTCGCACAGCCTATCCCTCCTGGGTCGTGGATGTGT606290.1168747661695041No Hit
GTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATA605240.11667235724889188No Hit
GGGTCTTCCGTACGCCACATGTCTCGCGCCCCGCCGCGGGGCGGGGATTC593590.11442658208209922No Hit
GGCAGGAAGGAAGTTACTAGGCTAATTTATATACGTCACGGCTTCTATTC593130.11433790769783102No Hit
GATCAGTCCATGTTGCATTACCAGCCTAAAATTGCCAAATATGATATAGGATGACATTCCTAAAATGGAGCGGTG592930.1142993536177144No Hit
GTCTTCCGTACGCCACATGTCTCGCGCCCCGCCGCGGGGCGGGGATTCGG576730.11117647312826881No Hit
GCTCAGACAGGCGTAGCCCCGGGAGGACCCCGGGGCCGCAAGTGCGTTCG562670.10846612129607096No Hit
CCCTCGTGGAGCCATTCATACAGGTCCCTAATTAAGGAACAAGTGATTAT556200.10721889680429855No Hit
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCACTGCTGTAGGGCTGGCCTAAGTC552250.10645745372199547No Hit
GGGCGTTCATGATTCAAGCGTCTAAGAGAGTAATGAGGATGCAAGGGTGAGAGAAACATTAGCACATGTGTGCA549790.10598323853656115No Hit
AGGGGCGACACGCAGAGTATGGTTTAGGGCCCTGGATCGTGCGAGAGAAA538910.10388589657821744No Hit
AGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCC536780.10347529562497552No Hit
CATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG534590.10305312844769861No Hit
ATGTGCGTCTAGGTCGGGGCAGGAAGGAAGTTACTAGGCTAATTTATATA533920.10292397227930797No Hit
GTTAAGACGCTTACTTGGAATGTTTACTTGGAGCTGGTGTACTTGGTGAC530490.10226276980530807No Hit
CTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTG522640.10074952216073103No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)