FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005284346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2239801
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA57122425.503337126825105No Hit
TCTTAACCCGGACCAGAAACTATGGAATTCTCGGGTGCCAAGGAACTCCA1745997.795290742347199RNA PCR Primer, Index 1 (100% over 28bp)
AGGTTCCGGATAAGTAAGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCA1626797.263100605812749RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGTATGC1313285.863378041174194RNA PCR Primer, Index 8 (100% over 50bp)
GTCCCACTCCGTAGATCTGTTCTGGAATTCTCGGGTGCCAAGGAACTCCA1042144.652824067852457RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA453282.0237512171840266No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGAATTCTCGGGTGCC176830.7894897805653269No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACT154190.6884093720826091RNA PCR Primer, Index 8 (100% over 36bp)
TGATACGGATGTTATACGCAGCTGGAATTCTCGGGTGCCAAGGAACTCCA143630.6412623264298927RNA PCR Primer, Index 1 (100% over 28bp)
TAGCATATCGAGCCTGAGAACATGGAATTCTCGGGTGCCAAGGAACTCCA130850.5842036859524574RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCGCGTATGC126060.5628178574793028RNA PCR Primer, Index 8 (98% over 50bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA115670.5164298078266775RNA PCR Primer, Index 1 (100% over 34bp)
CCTGGAACTTAGGACGTGAATCTGGAATTCTCGGGTGCCAAGGAACTCCA115040.513617057943987RNA PCR Primer, Index 1 (100% over 28bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG112560.5025446457073642No Hit
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA90060.4020892927541331RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGTATGG76180.34011950168787314RNA PCR Primer, Index 8 (97% over 49bp)
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA63590.2839091508575985RNA PCR Primer, Index 1 (100% over 23bp)
TTCTTACTCGTCTATCGGCTAGGATGCGCGCCTTGGAATTCTCGGGTGCC58040.2591301637958015No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATGTCGTATGC55080.24591470402950977RNA PCR Primer, Index 8 (98% over 50bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC52730.23542270049883895RNA PCR Primer, Index 5 (100% over 35bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG52000.23216348238080078RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC43270.19318680543494712RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAAGCTCGTATGC41860.18689160331654464RNA PCR Primer, Index 8 (98% over 50bp)
TCTTACTCGTCTATCGGCTAGGATGCGCGCCTTGGAATTCTCGGGTGCCA36500.16296090590190826No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACATGGAATTCTCGGGTGCCA35320.15769258072480546No Hit
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGTATGCC35240.1573354061365273RNA PCR Primer, Index 8 (98% over 50bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC34970.15612994190108853RNA PCR Primer, Index 1 (100% over 26bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTT34960.15608529507755375RNA PCR Primer, Index 8 (100% over 37bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGGATGC34740.15510306495978884RNA PCR Primer, Index 8 (98% over 50bp)
GTCCGACGATCGTCCCACTCCGTAGATCTGTTCTGGAATTCTCGGGTGCC33200.14822745413543434No Hit
GCTCTACACTCTACTCGTCAGCTGTTGGAATTCTCGGGTGCCAAGGAACT32540.14528076378213958RNA PCR Primer, Index 1 (100% over 25bp)
CGCCCGTGAAAGTAGGACATCGTCAGACATGGAATTCTCGGGTGCCAAGG32510.14514682331153525Illumina Small RNA Adapter 2 (100% over 21bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATATCGTATGC31150.13907485531080663RNA PCR Primer, Index 8 (98% over 50bp)
CCCGTGAAAGTAGGACATCGTCAGACATGGAATTCTCGGGTGCCAAGGAA29390.1312170143686872RNA PCR Primer, Index 1 (100% over 23bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA28410.12684162566227983RNA PCR Primer, Index 1 (100% over 28bp)
CGTTCCCGTGGAATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCA26460.1181354950729998No Hit
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC24880.11108129695450622RNA PCR Primer, Index 1 (100% over 27bp)
TATGCATATGATCACGAGACTCAGTTGGAATTCTCGGGTGCCAAGGAACT23970.10701843601284222RNA PCR Primer, Index 1 (100% over 25bp)
CTTACTCGTCTATCGGCTAGGATGCGCGCCTTGGAATTCTCGGGTGCCAA23180.10349133695359543No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)