FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005284347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7504850
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA367676048.9917853121648No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA2913823.88258259658754No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGAATTCTCGGGTGCC1245011.658940551776518No Hit
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA1159421.5448943016849104RNA PCR Primer, Index 1 (100% over 28bp)
TCTTAACCCGGACCAGAAACTATGGAATTCTCGGGTGCCAAGGAACTCCA1020581.3598939352552015RNA PCR Primer, Index 1 (100% over 28bp)
AGGTTCCGGATAAGTAAGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCA947131.262023891217013RNA PCR Primer, Index 1 (100% over 28bp)
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA864941.1525080447976974RNA PCR Primer, Index 1 (100% over 23bp)
GTCCCACTCCGTAGATCTGTTCTGGAATTCTCGGGTGCCAAGGAACTCCA726420.9679340693018514RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG547710.7298080574561784RNA PCR Primer, Index 1 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACATGGAATTCTCGGGTGCCA507270.675922903189271No Hit
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC492630.6564155179650493RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGC410220.5466065277787031RNA PCR Primer, Index 7 (100% over 50bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC350090.46648500636255225RNA PCR Primer, Index 1 (100% over 26bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC289530.3857905221290232RNA PCR Primer, Index 1 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC281410.37497085218225545RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA275070.36652298180509935RNA PCR Primer, Index 1 (100% over 28bp)
TGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA274440.365683524654057No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTTGGAATTCTCGGGT232980.31043924928546207No Hit
GCTCTACACTCTACTCGTCAGCTGTTGGAATTCTCGGGTGCCAAGGAACT221770.295502241883582RNA PCR Primer, Index 1 (100% over 25bp)
CGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA218400.29101181236133966No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTTGGAATTCTCGGGTGCCAAGGA200350.2669606987481429RNA PCR Primer, Index 1 (100% over 22bp)
AGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA196070.26125772000772834No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA178940.238432480329387RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA169380.22569405118023678RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC156890.20905148004290558RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC149630.199377735730894RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA138640.18473387209604455RNA PCR Primer, Index 1 (100% over 28bp)
TATGCATATGATCACGAGACTCAGTTGGAATTCTCGGGTGCCAAGGAACT136540.18193568159257015RNA PCR Primer, Index 1 (100% over 25bp)
GGCAGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA133600.17801821488770594No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTATGGAATTCTCGGG126530.1685976401926754No Hit
CGATGCTATAGACTCTCACGTGATGTGGAATTCTCGGGTGCCAAGGAACT123500.1645602510376623RNA PCR Primer, Index 1 (100% over 25bp)
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTGCCAAGGAACTCC108900.14510616468017348RNA PCR Primer, Index 1 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATGGAATTCTCGGGTGC108210.1441867592290319No Hit
GGATGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA108160.14412013564561585No Hit
CCCCCCACTGCTAAATTTGACTGGCTATGGAATTCTCGGGTGCCAAGGAA99820.13300732193181744RNA PCR Primer, Index 1 (100% over 23bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC94640.12610511868991386RNA PCR Primer, Index 1 (100% over 27bp)
TAGCATATCGAGCCTGAGAACATGGAATTCTCGGGTGCCAAGGAACTCCA94260.1255987794559518RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA94000.12525233682218834RNA PCR Primer, Index 1 (100% over 28bp)
TGATACGGATGTTATACGCAGCTGGAATTCTCGGGTGCCAAGGAACTCCA88400.11779049547958986RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC87910.11713758436211251RNA PCR Primer, Index 1 (100% over 27bp)
CCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAAC83100.11072839563748776RNA PCR Primer, Index 1 (100% over 24bp)
TCCGTTGTCGTCCAGCGGTTAGGATATCTGGCTTGGAATTCTCGGGTGCC83040.11064844733738849No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTATCGGGTGCCA82120.10942257340253302No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA77470.10322658014483967RNA PCR Primer, Index 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)