FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005284348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6031589
Sequences flagged as poor quality0
Sequence length76
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTAACCCGGACCAGAAACTATGGAATTCTCGGGTGCCAAGGAACTCCA87633114.529023777979566RNA PCR Primer, Index 1 (100% over 28bp)
AGGTTCCGGATAAGTAAGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCA73530212.190850537064115RNA PCR Primer, Index 1 (100% over 28bp)
GTCCCACTCCGTAGATCTGTTCTGGAATTCTCGGGTGCCAAGGAACTCCA5791789.602411570151746RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA4696197.785991386349435No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGTATGC964551.599164001393331RNA PCR Primer, Index 8 (100% over 50bp)
TAGCATATCGAGCCTGAGAACATGGAATTCTCGGGTGCCAAGGAACTCCA768541.274191593624831RNA PCR Primer, Index 1 (100% over 28bp)
TGATACGGATGTTATACGCAGCTGGAATTCTCGGGTGCCAAGGAACTCCA682431.1314265610604435RNA PCR Primer, Index 1 (100% over 28bp)
CCTGGAACTTAGGACGTGAATCTGGAATTCTCGGGTGCCAAGGAACTCCA666041.1042529588803216RNA PCR Primer, Index 1 (100% over 28bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACT367540.6093584957463116RNA PCR Primer, Index 8 (100% over 36bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA330500.5479484759322958No Hit
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA261250.4331362763610054RNA PCR Primer, Index 1 (100% over 28bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG236820.392632853465314No Hit
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA227410.37703165782681813RNA PCR Primer, Index 1 (100% over 34bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATGTCGTATGC169280.280655727702932RNA PCR Primer, Index 8 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCGCGTATGC160460.2660327154254045RNA PCR Primer, Index 8 (98% over 50bp)
CTCTATGCTGAGAACTGATAGCAAGCTTGGAATTCTCGGGTGCCAAGGAA143180.23738354851433013RNA PCR Primer, Index 1 (100% over 23bp)
AGGTTCAGGATAAGTAAGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCA137640.2281985725486269RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGTATGG134710.22334081450178386RNA PCR Primer, Index 8 (97% over 49bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG129210.21422215605207848RNA PCR Primer, Index 1 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGAATTCTCGGGTGCC127150.21080680397818882No Hit
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC123590.20490454505437952RNA PCR Primer, Index 1 (100% over 27bp)
TCTTAACCCGGACCAGAAACTATGGAATTCTCGGGTGCAAAGGAACTCCA105720.1752771947823368RNA PCR Primer, Index 1 (96% over 28bp)
TCTTAACCCGGACCAGAAACTATGGAATTCTCGGGTGCCAAGGAAATCCA94070.15596221824796086RNA PCR Primer, Index 1 (96% over 28bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC92960.15412190717902033RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA88280.14636275780727104RNA PCR Primer, Index 1 (100% over 28bp)
AGGTTCCGGATAAGTAAGAGCCTGGAATTCTCGGGTGCAAAGGAACTCCA87580.1452022012773085RNA PCR Primer, Index 1 (96% over 28bp)
CATCGGTCGAACTTATGTGAAATGGAATTCTCGGGTGCCAAGGAACTCCA84690.14041076074646333RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGTAAGC84090.13941599800649546RNA PCR Primer, Index 8 (98% over 50bp)
AGGTTCCGGATAAGTAAGAGCCTGGAATTCTCGGGTGCCAAGGAAATCCA83080.13774148072754958RNA PCR Primer, Index 1 (96% over 28bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC82660.13704514680957208RNA PCR Primer, Index 5 (100% over 35bp)
CCCGTGAAAGTAGGACATCGTCAGACATGGAATTCTCGGGTGCCAAGGAA81480.13508878008763528RNA PCR Primer, Index 1 (100% over 23bp)
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA81400.13495614505563955RNA PCR Primer, Index 1 (100% over 23bp)
GAATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCA80580.13359663597768348RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAAGCTCGTATGC80540.13353031846168564RNA PCR Primer, Index 8 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGGATGC79740.1322039681417285RNA PCR Primer, Index 8 (98% over 50bp)
CGCCCGTGAAAGTAGGACATCGTCAGACATGGAATTCTCGGGTGCCAAGG79420.13167342801374562Illumina Small RNA Adapter 2 (100% over 21bp)
TCATGAGTCCGTACCTTGATTGTGGAATTCTCGGGTGCCAAGGAACTCCA78980.1309439353377692RNA PCR Primer, Index 1 (100% over 28bp)
TCTTAAACCGGACCAGAAACTATGGAATTCTCGGGTGCCAAGGAACTCCA76980.12762805953787634RNA PCR Primer, Index 1 (100% over 28bp)
TCTTAACCCGGACCAGAAACTATGGAATTCTCGGGTGACAAGGAACTCCA73380.12165948309806919RNA PCR Primer, Index 1 (96% over 28bp)
TTCTTACTCGTCTATCGGCTAGGATGCGCGCCTTGGAATTCTCGGGTGCC73010.121046046075089No Hit
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTT70000.11605565299625023RNA PCR Primer, Index 8 (100% over 37bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC68560.11366822242032737RNA PCR Primer, Index 1 (100% over 27bp)
TCTTAACCCGGCCCAGAAACTATGGAATTCTCGGGTGCCAAGGAACTCCA68090.11288899160735255RNA PCR Primer, Index 1 (100% over 28bp)
TCTTACTCGTCTATCGGCTAGGATGCGCGCCTTGGAATTCTCGGGTGCCA67880.1125408246483638No Hit
CGTTCCCGTGGAATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCA63360.10504694534060594No Hit
TCTTAACCCGGACCAGAAACTATGGAATTCTAGGGTGCCAAGGAACTCCA62760.10405218260063807RNA PCR Primer, Index 1 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)