FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005284370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10095281
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA185410618.366066283840933No Hit
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA2354582.332357068614534RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA2031462.012286730800262No Hit
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC2022132.0030447889464393RNA PCR Primer, Index 2 (100% over 34bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC1200081.1887534383639247RNA PCR Primer, Index 1 (100% over 27bp)
GGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCAAGGA925330.9165965761626645RNA PCR Primer, Index 1 (100% over 22bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG911150.9025504094437787RNA PCR Primer, Index 1 (100% over 29bp)
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA855570.8474949830519823RNA PCR Primer, Index 1 (100% over 23bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA823900.8161238899640337RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG807280.7996607523851985Illumina Small RNA Adapter 2 (100% over 21bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA701840.695215913256897RNA PCR Primer, Index 1 (100% over 22bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC691570.6850428432848972RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC634270.6282836505492021RNA PCR Primer, Index 1 (100% over 27bp)
AAGATTAAGAGAACCAACACCTTGGAATTCTCGGGTGCCAAGGAACTCCA596900.5912663550425193RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC557630.5523669920629253RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA496850.4921606441663189RNA PCR Primer, Index 1 (100% over 28bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC491220.4865837810755342RNA PCR Primer, Index 1 (100% over 24bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA466170.4617702072879397RNA PCR Primer, Index 1 (100% over 23bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA332420.32928256281325896RNA PCR Primer, Index 1 (100% over 28bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT326640.3235571154482971RNA PCR Primer, Index 1 (97% over 35bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGAATTCTCGGGTGCC324560.3214967468463731No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG318120.31511752867503146No Hit
AAAGATTAAGAGAACCAACACCTTGGAATTCTCGGGTGCCAAGGAACTCC313920.31095716899806947RNA PCR Primer, Index 1 (100% over 27bp)
CTTTGGACACTAGGAAAAAACCTGGAATTCTCGGGTGCCAAGGAACTCCA299730.2969010966609052RNA PCR Primer, Index 1 (100% over 28bp)
GGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAAGGAA296230.29343413026343695RNA PCR Primer, Index 1 (100% over 23bp)
GCAGCCGACTTAGAACTGGTGCGGACCTGGAATTCTCGGGTGCCAAGGAA282840.28017050738855115RNA PCR Primer, Index 1 (100% over 23bp)
CGGACAGGATTGACAGATTGATAGCTTGGAATTCTCGGGTGCCAAGGAAC253210.2508201604294125RNA PCR Primer, Index 1 (100% over 24bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCC241340.2390621915328558No Hit
CGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTT240870.2385966274737672RNA PCR Primer, Index 12 (100% over 36bp)
CGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTCTCGGGTGCCAAGG235980.2337527801355901Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCTGGAATTCTCGGGTGCCAAGGA215120.21308966040667912RNA PCR Primer, Index 1 (100% over 22bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACATGGAATTCTCGGGTGCCA206740.2047887522893122No Hit
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGC206610.20465997925169194No Hit
CCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAAC202100.2001925454080971RNA PCR Primer, Index 1 (100% over 24bp)
CAAACGAGAACTTTGAAGGCCGAAGTGGAATTCTCGGGTGCCAAGGAACT200650.19875623075771737RNA PCR Primer, Index 1 (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG189960.18816712481802142No Hit
CGCGACCTCAGATCAGACGTGGCGACCTGGAATTCTCGGGTGCCAAGGAA159150.15764791490202204RNA PCR Primer, Index 1 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCAA150680.1492578562201488No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC148750.147346071892402RNA PCR Primer, Index 1 (100% over 26bp)
GTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCAAGGAA144230.14286873243052867RNA PCR Primer, Index 1 (100% over 23bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTT143930.14257156388217426RNA PCR Primer, Index 12 (100% over 36bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA141490.14015459302222494RNA PCR Primer, Index 1 (100% over 28bp)
ACAGAGTGGTTCATTTCCACCTTTCGGGCGCTGGAATTCTCGGGTGCCAA134610.13333952764663015No Hit
CGGCTCGAAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTG133340.1320815141252631RNA PCR Primer, Index 12 (100% over 37bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA129180.12796077692141505RNA PCR Primer, Index 1 (100% over 28bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC128280.1270692712763518RNA PCR Primer, Index 1 (100% over 33bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG122150.12099712727164307RNA PCR Primer, Index 1 (100% over 29bp)
CGAAGAAGCAGCTTCAAACCTGCCGGGGCTTTGGAATTCTCGGGTGCCAA122110.12095750479852914No Hit
TTCGACCTGAGCTCAGGCAAGGCTACCCGTGGAATTCTCGGGTGCCAAGG112260.11120047079422554Illumina Small RNA Adapter 2 (100% over 21bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA108340.10731746842906106No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC104840.10385050203159278RNA PCR Primer, Index 1 (100% over 27bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA104020.10303824133275737RNA PCR Primer, Index 1 (100% over 32bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC102740.1017703221931118RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC102350.10138400308025106RNA PCR Primer, Index 1 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTGGT2352650.069.9043661
GCTGGTC2366650.069.8192442
TGGTCCG2382550.069.779764
CTGGTCC2364150.069.770513
CTACGGC187950.069.589393
GTCTACG188250.069.571141
TACGGCC188800.069.442984
CAACGCT148250.069.2727057
TCAACGC148850.069.100546
ACGGCCA189900.069.07765
TGCCCCG641250.069.0541614
TCCTGTA645000.069.051331
TCTACGG189800.069.021422
GCCCCGA634550.069.0052315
CCTGTAC652300.068.992372
ACTGAGC643900.068.9119957
CTGCCCC642550.068.89879613
CAGTTGA102650.068.874258
GCTGCCC641950.068.8541412
ACGCTGT150900.068.839739
Produced by FastQC (version 0.11.5)