FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005284372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10710290
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA173174016.168936602090138No Hit
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC7691787.181672951899529RNA PCR Primer, Index 2 (100% over 34bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT2301382.148756009407775RNA PCR Primer, Index 1 (97% over 35bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA1768241.6509730362109711RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA1721761.607575518496698No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1257221.173843098552887RNA PCR Primer, Index 1 (100% over 28bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTT997760.9315900876633593RNA PCR Primer, Index 12 (100% over 36bp)
CGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTT977820.9129724778694134RNA PCR Primer, Index 12 (100% over 36bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA902020.8421994175694589RNA PCR Primer, Index 1 (100% over 22bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG812550.7586629306956207RNA PCR Primer, Index 1 (100% over 29bp)
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA742400.6931651710644624RNA PCR Primer, Index 1 (100% over 23bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC738830.6898319279870107RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC733920.6852475516535967RNA PCR Primer, Index 1 (100% over 27bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC676890.6319996937524567RNA PCR Primer, Index 1 (100% over 33bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG656690.6131393267595928Illumina Small RNA Adapter 2 (100% over 21bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA587700.5487246377082227RNA PCR Primer, Index 1 (100% over 23bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA551890.5152895019649327RNA PCR Primer, Index 1 (100% over 32bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGC501900.4686147620652662RNA PCR Primer, Index 12 (100% over 50bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC485650.4534424371328881RNA PCR Primer, Index 1 (100% over 24bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG458610.4281956884454109No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCTGGAATTCTCGGGTGCCAAGGAACT429060.40060539910684023RNA PCR Primer, Index 1 (100% over 25bp)
CGCGACCTCAGATCAGACGTGGCGACCTGGAATTCTCGGGTGCCAAGGAA410400.3831829016767987RNA PCR Primer, Index 1 (100% over 23bp)
GCAGCCGACTTAGAACTGGTGCGGACCTGGAATTCTCGGGTGCCAAGGAA396560.370260749242084RNA PCR Primer, Index 1 (100% over 23bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGAATTCTCGGGTGCC365160.340943149065058No Hit
GGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCAAGGA290740.2714585692824377RNA PCR Primer, Index 1 (100% over 22bp)
CGGCTCGAAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTG287620.26854548289542113RNA PCR Primer, Index 12 (100% over 37bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACATGGAATTCTCGGGTGCCA279210.26069322119195654No Hit
CCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAAC256270.2392745667951101RNA PCR Primer, Index 1 (100% over 24bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC255840.23887308373536104RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTCTCGGGTGCCAAGG253690.23686566843661563Illumina Small RNA Adapter 2 (100% over 21bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA250480.233868550711512RNA PCR Primer, Index 1 (100% over 28bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCC244630.2284065137358559No Hit
CAAACGAGAACTTTGAAGGCCGAAGTGGAATTCTCGGGTGCCAAGGAACT241210.22521332288854926RNA PCR Primer, Index 1 (100% over 25bp)
ACAGAGTGGTTCATTTCCACCTTTCGGGCGCTGGAATTCTCGGGTGCCAA234480.21892964616270894No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC226460.21144152025762142RNA PCR Primer, Index 1 (100% over 27bp)
CGAAGAAGCAGCTTCAAACCTGCCGGGGCTTTGGAATTCTCGGGTGCCAA217850.20340252224729677No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCAA203210.18973342458514197No Hit
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTG195540.18257208721705948RNA PCR Primer, Index 12 (100% over 37bp)
CGCGACCTCAGATCAGACGTGGCGACCCTGGAATTCTCGGGTGCCAAGGA193170.18035926198076804RNA PCR Primer, Index 1 (100% over 22bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGC171950.16054653982291797No Hit
CTTTGGACACTAGGAAAAAACCTGGAATTCTCGGGTGCCAAGGAACTCCA150170.14021095600585978RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA142810.13333905991341038No Hit
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG142640.13318033405257934RNA PCR Primer, Index 12 (100% over 45bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA141670.13227466296430815RNA PCR Primer, Index 1 (100% over 28bp)
CGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGT138150.12898810396357147RNA PCR Primer, Index 12 (100% over 46bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC136500.12744752943197615RNA PCR Primer, Index 1 (100% over 31bp)
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTC135270.12629910114478693RNA PCR Primer, Index 1 (100% over 26bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTTGGAATTCTC131850.12310591029748028No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC131100.12240564914675514No Hit
CTAGACTGAAGCTCCTTGAGGATGGAATTCTCGGGTGCCAAGGAACTCCA127370.11892301702381541RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG126420.11803601956623025No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT117070.10930609722052344No Hit
GGGTTCGTTTCCCGGTCAGGGAATGGAATTCTCGGGTGCCAAGGAACTCC116920.10916604499037841RNA PCR Primer, Index 1 (100% over 27bp)
GAAAAAGTCATGGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA115070.10743873415192307RNA PCR Primer, Index 1 (100% over 32bp)
CGAAGAAGCAGCTTCAAACCTGCCGGGGCTTGGAATTCTCGGGTGCCAAG114570.10697189338477298No Hit
CACCACGATCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC111760.10434824827338941RNA PCR Primer, Index 2 (100% over 34bp)
GGGGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC107640.10050148035207264RNA PCR Primer, Index 12 (100% over 44bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC107340.10022137589178258RNA PCR Primer, Index 1 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTA207.9361824E-569.99187518
GGCTGGT2073400.069.899611
GCTGGTC2078450.069.8011252
GTCTACG199650.069.796591
TACGGCC200700.069.727224
TGGTCCG2088500.069.64094
TCCTGTA430950.069.6183551
CTGGTCC2085150.069.602033
CTACGGC201200.069.5716553
GGCCATA201750.069.453827
TCTACGG200400.069.412732
CCTGTAC435050.069.346832
ACGGCCA203150.069.334285
CGGCCAT202250.069.262716
CAGTTGA145500.069.246588
TGCCCCG434200.069.1794414
TGCCAGT145600.069.116765
GCCAGTT145250.069.0911566
CTGTACT416300.069.048053
CAACGCT64000.069.00737
Produced by FastQC (version 0.11.5)