FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005284373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5935863
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA251845542.42778177326532No Hit
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA2046353.447434686413753RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA1939743.267831484655222No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA1201262.0237326905961273RNA PCR Primer, Index 1 (100% over 23bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG963541.6232517495771044RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC876271.476230162320121RNA PCR Primer, Index 1 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGAATTCTCGGGTGCC737671.24273420730903No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC555850.9364265987944802RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA533860.8993805955427207RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC501500.8448645125401311RNA PCR Primer, Index 1 (100% over 27bp)
TCTTAACCCGGACCAGAAACTATGGAATTCTCGGGTGCCAAGGAACTCCA392570.6613528647814142RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC359760.6060786780287888RNA PCR Primer, Index 1 (100% over 26bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACATGGAATTCTCGGGTGCCA339330.5716607677771539No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA304580.5131183115243731RNA PCR Primer, Index 1 (100% over 28bp)
GCTCTACACTCTACTCGTCAGCTGTTGGAATTCTCGGGTGCCAAGGAACT258960.4362634380207225RNA PCR Primer, Index 1 (100% over 25bp)
AGGTTCCGGATAAGTAAGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCA253160.4264923230202584RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA212480.3579597440170031RNA PCR Primer, Index 1 (100% over 28bp)
GTCCCACTCCGTAGATCTGTTCTGGAATTCTCGGGTGCCAAGGAACTCCA209410.35278779176675745RNA PCR Primer, Index 1 (100% over 28bp)
TATGCATATGATCACGAGACTCAGTTGGAATTCTCGGGTGCCAAGGAACT204630.3447350452663749RNA PCR Primer, Index 1 (100% over 25bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA191080.32190769901529065RNA PCR Primer, Index 1 (100% over 28bp)
TGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA185760.3129452280148649No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC172080.2898988740137702RNA PCR Primer, Index 1 (100% over 26bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTTGGAATTCTCGGGT169510.2855692592635645No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA165930.27953812276327805RNA PCR Primer, Index 1 (100% over 28bp)
CGATGCTATAGACTCTCACGTGATGTGGAATTCTCGGGTGCCAAGGAACT162230.273304825262982RNA PCR Primer, Index 1 (100% over 25bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC154390.2600969732623546RNA PCR Primer, Index 1 (100% over 26bp)
CGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA148550.25025847126188727No Hit
AGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA137150.23105317626097502No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC130740.22025440951046207RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA122540.2064400745098059RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC121320.20438477100970828RNA PCR Primer, Index 1 (100% over 27bp)
CCCCCCACTGCTAAATTTGACTGGCTTTTGGAATTCTCGGGTGCCAAGGA120090.20231262075960985RNA PCR Primer, Index 1 (100% over 22bp)
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTGCCAAGGAACTCC117550.1980335462594066RNA PCR Primer, Index 1 (100% over 27bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA116990.19709012825936179RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTATGGAATTCTCGGG109040.1836969620087256No Hit
TGAGGTAGTAGATTGTATAGTTTTGGAATTCTCGGGTGCCAAGGAACTCC105320.17742997100842792RNA PCR Primer, Index 1 (100% over 27bp)
CCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAAC100510.16932668425804304RNA PCR Primer, Index 1 (100% over 24bp)
GGCAGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA90980.1532717315072804No Hit
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG89170.15022246975713557RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTC87140.14680257950697312RNA PCR Primer, Index 1 (100% over 26bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTCGTATGC86680.14602762900693633RNA PCR Primer, Index 15 (100% over 50bp)
CCCCCCACTGCTAAATTTGACTGGCTATGGAATTCTCGGGTGCCAAGGAA82130.1383623577565722RNA PCR Primer, Index 1 (100% over 23bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATGGAATTCTCGGGTGC81400.1371325450065138No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA77520.1305960060062033RNA PCR Primer, Index 1 (100% over 28bp)
CTAGACTGAAGCTCCTTGAGGATGGAATTCTCGGGTGCCAAGGAACTCCA77410.1304106917561945RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTC74520.12554198100596325RNA PCR Primer, Index 1 (100% over 26bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA72850.12272857375582961RNA PCR Primer, Index 1 (100% over 28bp)
GGATGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA72480.1221052440058No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA70120.11812941100561114RNA PCR Primer, Index 1 (100% over 28bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCCA65940.11108746950527666RNA PCR Primer, Index 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTAC206.611964E-572.6189343
GCGTTCC150.001923661972.6189343
CGCTCAC150.001923661972.6189343
CGGTACA206.611964E-572.6189343
GTGACCC206.611964E-572.6189345
AGGCTAC206.611964E-572.6189342
GCATTGA208.0690705E-569.7570651
GTATGCT150.002256255469.7570651
CGCACGA150.002256255469.7570651
CTGTGCG252.9017501E-669.757061
GTGCATC4800.069.725924
ACTTCCG150.002260254769.725924
TACGGCC1750.069.725924
ATGGGGT14800.069.7259145
GCGCGAC208.086936E-569.7259143
ACGTCCG353.8344297E-969.7259144
TGCTACG252.9094554E-669.7259144
TGCTGCG6450.069.7235715
TACTTCG301.0535041E-769.72356415
GCCTCTC252.9100374E-669.72356415
Produced by FastQC (version 0.11.5)