FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005284374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7845955
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA113936314.521661161706891No Hit
TCTTAACCCGGACCAGAAACTATGGAATTCTCGGGTGCCAAGGAACTCCA6555638.355426458601917RNA PCR Primer, Index 1 (100% over 28bp)
AGGTTCCGGATAAGTAAGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCA5169376.588579720378207RNA PCR Primer, Index 1 (100% over 28bp)
GTCCCACTCCGTAGATCTGTTCTGGAATTCTCGGGTGCCAAGGAACTCCA3863454.924129694855503RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTATGC1227171.5640798347683615RNA PCR Primer, Index 16 (100% over 50bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA1127361.43686778728657No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATGTCGTATGC549070.6998128334919076RNA PCR Primer, Index 16 (98% over 50bp)
TAGCATATCGAGCCTGAGAACATGGAATTCTCGGGTGCCAAGGAACTCCA547040.6972255130191289RNA PCR Primer, Index 1 (100% over 28bp)
CCTGGAACTTAGGACGTGAATCTGGAATTCTCGGGTGCCAAGGAACTCCA526760.6713777991334389RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCAGGTCGTATGC505070.6437329808799566RNA PCR Primer, Index 16 (97% over 40bp)
TGATACGGATGTTATACGCAGCTGGAATTCTCGGGTGCCAAGGAACTCCA424360.540864687600171RNA PCR Primer, Index 1 (100% over 28bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG370830.4726384487293134No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCAGCTCGTATGC326130.41566641664399046RNA PCR Primer, Index 16 (98% over 50bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCG257520.3282200828324914RNA PCR Primer, Index 7 (97% over 36bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA235910.30067722794739454RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTTGGAATTCTCGGG192450.24528562807204476No Hit
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC189840.24195907317847223RNA PCR Primer, Index 2 (100% over 34bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCGCGTATGC175440.22360566686910643RNA PCR Primer, Index 16 (98% over 50bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG164700.2099170846633711No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC143640.18307522793592365No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT140930.1796212188318694No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGAATTCTCGGGTGCC136980.17458677751784202No Hit
ATAGGCGATAGAAATTGAAACCTTGGAATTCTCGGGTGCCAAGGAACTCC134170.17100531420330606RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG133330.16993469883525972RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC101420.12926406027054704RNA PCR Primer, Index 1 (100% over 27bp)
GCTCTACACTCTACTCGTCAGCTGTTGGAATTCTCGGGTGCCAAGGAACT100050.12751793758694766RNA PCR Primer, Index 1 (100% over 25bp)
AGGTTCAGGATAAGTAAGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCA95480.12169328016793367RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCAGGTCGGATGC87940.11208323269761297RNA PCR Primer, Index 16 (97% over 40bp)
CCCGTGAAAGTAGGACATCGTCAGACATGGAATTCTCGGGTGCCAAGGAA87580.11162439753987882RNA PCR Primer, Index 1 (100% over 23bp)
CGCCCGTGAAAGTAGGACATCGTCAGACATGGAATTCTCGGGTGCCAAGG84320.1074693902781752Illumina Small RNA Adapter 2 (100% over 21bp)
GTCCGACGATCGTCCCACTCCGTAGATCTGTTCTGGAATTCTCGGGTGCC82550.10521345075264897No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCAGGGCGTATGC82010.10452519801604776RNA PCR Primer, Index 16 (97% over 40bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACATGGAATTCTCGGGTGCCA81010.10325065591123068No Hit
TGTAGTATTCTCTGATGGTAGTTTGTATTTCTGTGGAATTCTCGGGTGCC80180.10219278596423252No Hit
TCTTAACCCGGACCAGAAACTATGGAATTCTCGGGTGCAAAGGAACTCCA79370.1011604068593307RNA PCR Primer, Index 1 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCCG150.00222483170.0044255
TCTTAAC812950.069.655681
TGGTCCG1499150.069.654214
AGGTTCC622800.069.63351
GGTTCCG620400.069.5925752
GGCTGGT1453600.069.568591
GCTGGTC1468900.069.5421452
GGTCCGA1514000.069.507375
GTCCGAT1509750.069.420196
CTTAACC815900.069.3308942
TCCGATG1511950.069.2966847
GGGTTAT1530350.069.29098519
TTAACCC815800.069.2407153
AGTGGGT1521250.069.21081516
GTTCCGG623900.069.190953
TGGGTTA1532200.069.179918
TTCCGGA626100.069.154674
GTTATCA1515650.069.1243321
GGTAGTG1524450.069.0673913
TTATCAG1515950.069.0598522
Produced by FastQC (version 0.11.5)