FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005284375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6240874
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA373457859.84062488683476No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA2947034.722143084446185No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGAATTCTCGGGTGCC1381262.213247695755434No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA881061.4117573916730253RNA PCR Primer, Index 1 (100% over 23bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA494020.7915878449076202RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACATGGAATTCTCGGGTGCCA429480.688172842457643No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTTGGAATTCTCGGGT259280.4154546302328808No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG252500.4045907672547146No Hit
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG242290.3882308792005735RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC224010.35894010999100445RNA PCR Primer, Index 1 (100% over 27bp)
CCCCCCACTGCTAAATTTGACTGGCTTTTGGAATTCTCGGGTGCCAAGGA194100.3110141303926341RNA PCR Primer, Index 1 (100% over 22bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA186920.29950933154554954RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTATGGAATTCTCGGG154530.24760954955988537No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC143840.23048053846304217RNA PCR Primer, Index 1 (100% over 26bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC135390.21694076823214184RNA PCR Primer, Index 1 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATGGAATTCTCGGGTGC118450.1897971341834493No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA114820.18398064117301519RNA PCR Primer, Index 1 (100% over 28bp)
TGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA110380.1768662530280214No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTATCGGGTGCCA108370.17364555028670664No Hit
GCTCTACACTCTACTCGTCAGCTGTTGGAATTCTCGGGTGCCAAGGAACT106870.17124204077826277RNA PCR Primer, Index 1 (100% over 25bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC106850.17120999398481687RNA PCR Primer, Index 1 (100% over 27bp)
CGATGCTATAGACTCTCACGTGATGTGGAATTCTCGGGTGCCAAGGAACT95000.15222226886811047RNA PCR Primer, Index 1 (100% over 25bp)
GGGTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA91510.14663010341179777No Hit
CCCCCCACTGCTAAATTTGACTGGCTATGGAATTCTCGGGTGCCAAGGAA86730.13897091977822337RNA PCR Primer, Index 1 (100% over 23bp)
CCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAAC84890.13602261478119893RNA PCR Primer, Index 1 (100% over 24bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGC83100.13315442676778924No Hit
GGATGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA80230.12855571190830004No Hit
TATGCATATGATCACGAGACTCAGTTGGAATTCTCGGGTGCCAAGGAACT76640.12280331248475775RNA PCR Primer, Index 1 (100% over 25bp)
AGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA74150.11881348670074096No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAACTTGGAATTCTC72890.11679453871364812No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAACTGGAATTCTCG68820.11027301624740381No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC65910.10561020780102273RNA PCR Primer, Index 1 (100% over 26bp)
CGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA65700.1052737164698406No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTGGT4526650.069.959941
GCTGGTC4561200.069.853962
TGGTCCG4628150.069.8485264
CTGGTCC4569400.069.841783
GGTCCGA4641350.069.810545
GTCCGAT4618550.069.7877356
TCCGATG4620650.069.7625057
TAGTGGG4637550.069.7198715
AGTGGGT4637250.069.71986416
CGATGGT4631000.069.71939
GGGTTAT4664500.069.7173319
TGGGTTA4665500.069.7108318
GATGGTA4637400.069.6994910
GGTAGTG4638800.069.6988313
TGGTAGT4637250.069.6972212
ATCAGAA4628650.069.68558524
TCAGAAC4620650.069.6842825
GTGGGTT4669400.069.67602517
GAACTTG3991150.069.6664328
ATGGTAG4641000.069.6620211
Produced by FastQC (version 0.11.5)