FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005284380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9894946
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA331774633.52970294127932No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA2431472.4572847593104603No Hit
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA1401901.4167838813875284RNA PCR Primer, Index 1 (100% over 28bp)
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA1016061.026847443129048RNA PCR Primer, Index 1 (100% over 23bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGAATTCTCGGGTGCC940640.950626713879995No Hit
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG650960.6578712001055893RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC553580.5594573229606307RNA PCR Primer, Index 1 (100% over 27bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG475230.48027548609158655No Hit
TCTTAACCCGGACCAGAAACTATGGAATTCTCGGGTGCCAAGGAACTCCA449210.45397923343896973RNA PCR Primer, Index 1 (100% over 28bp)
AGGTTCCGGATAAGTAAGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCA389670.3938071011201072RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC358520.36232638359016817RNA PCR Primer, Index 1 (100% over 26bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC334560.33811200182396145RNA PCR Primer, Index 1 (100% over 27bp)
GTCCCACTCCGTAGATCTGTTCTGGAATTCTCGGGTGCCAAGGAACTCCA313410.31673745364552774RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACATGGAATTCTCGGGTGCCA241410.24397303431468953No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTTGGAATTCTCGGGTGCCAAGGA219140.22146659516888725RNA PCR Primer, Index 1 (100% over 22bp)
GCTCTACACTCTACTCGTCAGCTGTTGGAATTCTCGGGTGCCAAGGAACT216490.2187884602907383RNA PCR Primer, Index 1 (100% over 25bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTATCGGGTGCCA195020.19709051469305644No Hit
GGCGCCGAATTGTGGTCTGTCGACTCTGGTGGAATTCTCGGGTGCCAAGG194410.1964740383626146Illumina Small RNA Adapter 2 (100% over 21bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC193790.19584745586282126RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA175320.177181361070591RNA PCR Primer, Index 1 (100% over 28bp)
GGCGCCGAATTGTGGTCTGTCGACTCTGGTATGGAATTCTCGGGTGCCAA170320.17212827639483833No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA167240.1690155762345747RNA PCR Primer, Index 1 (100% over 28bp)
TGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA152560.1541797196265649No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC150790.15239092765134848RNA PCR Primer, Index 1 (100% over 26bp)
GGCAGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA127100.12844941245763242No Hit
GGCGCCGAATTGTGGTCTGTCGACTCTGTGGAATTCTCGGGTGCCAAGGA120740.12202188875007502RNA PCR Primer, Index 1 (100% over 22bp)
GGATGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA116440.11767623592892776No Hit
CGATGCTATAGACTCTCACGTGATGTGGAATTCTCGGGTGCCAAGGAACT107080.1082168614159188RNA PCR Primer, Index 1 (100% over 25bp)
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTGCCAAGGAACTCC104080.1051850106104672RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC103520.1046190651267829RNA PCR Primer, Index 1 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTGGT4162750.069.904451
TGGTCCG4315450.069.7335744
CTGGTCC4220100.069.7299653
GCTGGTC4214050.069.701332
GTCCGAT4324200.069.680246
GGTCCGA4359550.069.638715
TCCGATG4329800.069.616627
AGTGGGT4353000.069.5995616
CGATGGT4345500.069.574169
GGGTTAT4399450.069.5728319
TAGTGGG4355600.069.55415
GTTATCA4341250.069.536321
TGGGTTA4399900.069.5342418
GATGGTA4356200.069.52930510
GTGGGTT4402650.069.5167617
ATCAGAA4335300.069.5066124
TGGTAGT4357000.069.49615512
GGTAGTG4360450.069.4750413
ATGGTAG4360850.069.4564711
TTATCAG4344050.069.4262322
Produced by FastQC (version 0.11.5)