FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005284382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28663868
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACT1203420.41983866238848155No Hit
GTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCC930460.3246107608366045No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTCTAATACTGGTG888480.30996514496926936No Hit
CTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTTCTCTTGCGTCT716530.24997673028636608No Hit
CTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATG708730.24725553438914805No Hit
CCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA603570.2105682317543466No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT598160.20868083818973768No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGC574230.2003323487255802No Hit
CTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGG470340.16408811260224893No Hit
GTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTG462430.16132854086545473No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTC436780.1523799928188338No Hit
GTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGT432910.151029861008291No Hit
CCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCAT428860.14961693236935086No Hit
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT390950.13639122256633335No Hit
GGCAATGCTTGTGGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTA387690.13525390222980374No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTAT362220.12636815101158017No Hit
CTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTA355560.12404466836087857No Hit
GTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTT352220.12287943832283905No Hit
CCCAGATTTATTGAAAATAATACAGCACTACAGAAAAAATTCAAACAGGT337840.11786266947642936No Hit
GGTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCT337270.11766381285317111No Hit
GTAAGATTTGCCGAGTTCCTTTTACTTTTTTTAACCTTTCCTTATGAGCA327600.11429022768315847No Hit
CGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTTCTCTTGCGTCTCTGTCTTCTTC326150.11378436434329099No Hit
CTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTG312170.10890714400443094No Hit
GGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTG310830.10843965650413963No Hit
CTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGG306550.10694648747335844No Hit
CCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATA304740.10631503047669631No Hit
GGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGAC302720.1056103105135706No Hit
GTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGGTGAAG302060.10538005547611369No Hit
CCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTT301800.10528934894620642No Hit
CTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTTC297670.10384851060575635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATCG253100.031.95075470
GCACTAC120050.027.00623325
CGCTACC28850.025.79131
CGACTTA237550.024.47643767
ACTTATC259700.024.2172169
AGCACTA136650.023.62384824
CGAGGCG134200.023.4095554
GATTTAT155150.023.2777065
CACTACA140600.023.1578826
TAATACA143000.023.10933918
TTATGCT444900.022.61471770
TATGCCG12200.022.23907746
GACTTAT267900.022.1447568
TGTACGC34000.022.0814236
AATAATA152700.021.93734616
TATTGAA157900.021.7211429
GTTTCGA267950.021.71418463
AGATTTA144250.021.7110884
ACCGCGT50000.021.6901476
GGCGTTT146800.021.60565257
Produced by FastQC (version 0.11.5)