FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005284387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9238004
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA327013335.39869651496146No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA2331722.5240517323872127No Hit
TCTTAACCCGGACCAGAAACTATGGAATTCTCGGGTGCCAAGGAACTCCA1934122.0936557290947264RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA1872112.0265308393458152RNA PCR Primer, Index 1 (100% over 28bp)
AGGTTCCGGATAAGTAAGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCA1424691.54220543745164RNA PCR Primer, Index 1 (100% over 28bp)
GTCCCACTCCGTAGATCTGTTCTGGAATTCTCGGGTGCCAAGGAACTCCA1195081.2936560754899002RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG848300.918271955716841RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC779900.8442299873435863RNA PCR Primer, Index 1 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGAATTCTCGGGTGCC773620.8374319820601939No Hit
ATTCCCGTGTGAAAGTAGGTAATCGTCAGGCTTGGAATTCTCGGGTGCCA741390.8025434931615097No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA726170.7860680727135428RNA PCR Primer, Index 1 (100% over 23bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA697640.755184778010488RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC520220.5631303039054757RNA PCR Primer, Index 1 (100% over 26bp)
CCCGTGTGAAAGTAGGTAATCGTCAGGCTTGGAATTCTCGGGTGCCAAGG485570.5256222015058664Illumina Small RNA Adapter 2 (100% over 21bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG479840.5194195629272297No Hit
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC473450.5125024843028861RNA PCR Primer, Index 1 (100% over 27bp)
GCTCTACACTCTACTCGTCAGCTGTTGGAATTCTCGGGTGCCAAGGAACT467990.5065921166520387RNA PCR Primer, Index 1 (100% over 25bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC399770.43274499556397683RNA PCR Primer, Index 1 (100% over 27bp)
GATTCCCGTGTGAAAGTAGGTAATCGTCAGGCTTGGAATTCTCGGGTGCC380560.41195046029423676No Hit
CCGTGTGAAAGTAGGTAATCGTCAGGCTTGGAATTCTCGGGTGCCAAGGA363390.39336419425668145RNA PCR Primer, Index 1 (100% over 22bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGC327780.354816906336044RNA PCR Primer, Index 12 (100% over 50bp)
TATGCATATGATCACGAGACTCAGTTGGAATTCTCGGGTGCCAAGGAACT283700.3071009711621688RNA PCR Primer, Index 1 (100% over 25bp)
TCCCGTGTGAAAGTAGGTAATCGTCAGGCTTGGAATTCTCGGGTGCCAAG281580.304806103136565No Hit
CGATGCTATAGACTCTCACGTGATGTGGAATTCTCGGGTGCCAAGGAACT275720.2984627415186224RNA PCR Primer, Index 1 (100% over 25bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA253710.27463724847921694RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA251500.27224495681101674RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA227460.24622201938860386RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC226810.24551840419207438RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC220080.23823328069570007RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTGCCAAGGAACTCC218720.23676110120757687RNA PCR Primer, Index 1 (100% over 27bp)
CGGATTCCCGTGTGAAAGTAGGTAATCGTCAGGCTTGGAATTCTCGGGTG206310.22332746337845275No Hit
GGATTCCCGTGTGAAAGTAGGTAATCGTCAGGCTTGGAATTCTCGGGTGC202950.21969031405485429No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACATGGAATTCTCGGGTGCCA199570.21603151503290105No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTATCGGGTGCCA188260.20378861061328835No Hit
CGTGTGAAAGTAGGTAATCGTCAGGCTTGGAATTCTCGGGTGCCAAGGAA172590.1868260719523395RNA PCR Primer, Index 1 (100% over 23bp)
TAGCATATCGAGCCTGAGAACATGGAATTCTCGGGTGCCAAGGAACTCCA170950.18505079668724975RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA167140.18092652915066934RNA PCR Primer, Index 1 (100% over 28bp)
TGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA166510.1802445636524946No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTTGGAATTCTCGGGTGCCAAGGA159250.17238572314971934RNA PCR Primer, Index 1 (100% over 22bp)
CCTGGAACTTAGGACGTGAATCTGGAATTCTCGGGTGCCAAGGAACTCCA150910.16335779893578742RNA PCR Primer, Index 1 (100% over 28bp)
TGATACGGATGTTATACGCAGCTGGAATTCTCGGGTGCCAAGGAACTCCA145760.15778300160943856RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTTGGAATTCTCGGGTGCCAAGGAACTCC142380.15412420258748535RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATGTCGTATGC137660.1490148737757637RNA PCR Primer, Index 12 (98% over 50bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA133110.1440895674000574RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA125560.13591680627113822RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC125420.13576525838265496RNA PCR Primer, Index 1 (100% over 27bp)
GGCAGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA121960.1320198605672827No Hit
GGATGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA116550.12616361716232208No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA115110.12460483888077988RNA PCR Primer, Index 1 (100% over 28bp)
TTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCC111760.12097851440635878No Hit
GTGAAAGTAGGTAATCGTCAGGCTTGGAATTCTCGGGTGCCAAGGAACTC110550.11966870765589624RNA PCR Primer, Index 1 (100% over 26bp)
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGC106480.11526299404070403No Hit
GTGTGAAAGTAGGTAATCGTCAGGCTTGGAATTCTCGGGTGCCAAGGAAC105610.11432123216227229RNA PCR Primer, Index 1 (100% over 24bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC98590.10672218803975404RNA PCR Primer, Index 1 (100% over 27bp)
GGGTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA94360.10214327683772383No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)