FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005284388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5907188
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA5290468.95597025183556No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA589960.9987154632627233No Hit
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA542690.918694309373597RNA PCR Primer, Index 1 (100% over 28bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA270320.45761198052271235RNA PCR Primer, Index 1 (100% over 34bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG212690.3600528711799929RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC171260.28991797789405044RNA PCR Primer, Index 1 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGAATTCTCGGGTGCC102330.17322963142530762No Hit
TTCGACCTGAGCTCAGGCAAGGCTACCCTGGAATTCTCGGGTGCCAAGGA95430.16154894680853227RNA PCR Primer, Index 1 (100% over 22bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG92550.15667353062066078Illumina Small RNA Adapter 2 (100% over 21bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA87230.1476675534958427RNA PCR Primer, Index 1 (100% over 28bp)
GTCCCACTCCGTAGATCTGTTCTGGAATTCTCGGGTGCCAAGGAACTCCA86460.14636405680672426RNA PCR Primer, Index 1 (100% over 28bp)
TCTTAACCCGGACCAGAAACTATGGAATTCTCGGGTGCCAAGGAACTCCA80400.1361053685780781RNA PCR Primer, Index 1 (100% over 28bp)
AGGTTCCGGATAAGTAAGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCA79660.13485265747425002RNA PCR Primer, Index 1 (100% over 28bp)
GGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCAAGGA76500.1295032424903355RNA PCR Primer, Index 1 (100% over 22bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC64630.10940907924379586RNA PCR Primer, Index 1 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTGGT669250.069.730531
GCTGGTC671650.069.572312
TGCCCCG119050.068.9588814
TGGTCCG683200.068.8935554
TACGGCC27900.068.369294
GGTCCGA688900.068.359095
CTGGTCC684500.068.353653
AGTGGGT684950.068.3170416
GTCCGAT684600.068.302786
GCCCCGA117900.068.2958615
GGGTTAT688650.068.2357119
TTATCAG683900.068.1877922
ATCAGAA685750.068.15414424
TATCAGA685550.068.127523
GTGGGTT691400.068.02894617
TAGTGGG688900.068.0116815
TGGGTTA691700.067.93931618
TCCGATG688150.067.887487
GTTATCA688050.067.80080421
GCTGCCC121550.067.7420812
Produced by FastQC (version 0.11.5)