FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005284389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34407517
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGT4415871.283402693661388No Hit
CTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACT4303591.2507702895271402No Hit
GGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGAC3844431.117322705965676No Hit
CTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA3786771.1005647399665601No Hit
GTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGG2923460.8496573583034196No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT2746420.7982034855929884No Hit
GTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATT2719030.7902430157921596No Hit
GGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTC2669120.7757374645778712No Hit
GTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGT2473890.7189969563918257No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGC2400170.6975714056902159No Hit
AGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGAC2385140.6932031741784797No Hit
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT2172310.6313475046746325No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTCTAATACTGGTG1990140.5784026786937285No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTC1939920.563807030888047No Hit
GGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCT1907180.5542916683002729No Hit
CGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATT1831820.532389477566777No Hit
GGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTT1800930.5234117881857037No Hit
ATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG1791080.5205490416527295No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG1676770.4873266501619399No Hit
GGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATT1567500.4555690548667025No Hit
GTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATA1471130.4275606403100811No Hit
CCCTCGTGGAGCCATTCATACAGGTCCCTAATTAAGGAACAAGTGATTAT1389430.4038158289655136No Hit
GTTCCTTTTACTTTTTTTAACCTTTCCTTATGAGCATGCCTGTGTTGGGT1286340.3738543528148224No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA1271370.36950355935303325No Hit
GTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCC1256680.36523414345766364No Hit
CATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG1246490.3622725813083228No Hit
GGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTG1143310.33228494808271114No Hit
CCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA1078110.31333560047358255No Hit
GTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTC1076360.31282699068346026No Hit
CTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGG1029860.2993125019744959No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT1004590.29196817660512964TruSeq Adapter, Index 13 (97% over 38bp)
AGCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGG978690.2844407517113194No Hit
GGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCG922300.26805189110274946No Hit
GGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTC902240.26222176973711875No Hit
GTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTA872890.2536916569713531No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGC868030.252279174925642No Hit
GCAGGTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATT866880.2519449456349902No Hit
CACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACA862090.250552807980884No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA816060.23717491732983811No Hit
GTCACTGGGCAGGCGGTGCCTCTAATACTGGTGATGCTAGAGGTGATGTT809860.23537298550197622No Hit
GGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAAT802320.2331816038919635No Hit
GTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTT792610.2303595461421991No Hit
GGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGAC788540.2291766650874575No Hit
CTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTA772030.22437829501036066No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGC760740.22109703527865726No Hit
CTCGTGGAGCCATTCATACAGGTCCCTAATTAAGGAACAAGTGATTATGC748900.21765592675577258No Hit
GCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTT740460.2152029743965541No Hit
CCTCGTGGAGCCATTCATACAGGTCCCTAATTAAGGAACAAGTGATTATG692620.2012990359054389No Hit
CCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTT684710.19900011965408604No Hit
GCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCG647350.18814202722038906No Hit
CTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTG640950.18628196855937032No Hit
GTCTCAAACATGATCTGGGTCATCTTCTCGCGGTTGGCCTTGGGGTTCAG619650.1800914608281673No Hit
AGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCC614000.17844937779148667No Hit
GGGAGGGATGCCTTCTTTATCCTGGATCTTGGCCTTCACATTTTCGATGG599000.17408986530472395No Hit
GCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG578810.16822196149754137No Hit
GCCAGATTTTCTCCATGTCGTCCCAGTTGGTGACGATGCCGTGCTCGATG576570.1675709409661848No Hit
GCCTTCTTTATCCTGGATCTTGGCCTTCACATTTTCGATGGTGTCACTGG575380.16722508630890162No Hit
CTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATG571230.1660189545208973No Hit
CAGCTGAACCCTCGTGGAGCCATTCATACAGGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCACGGTT556990.16188032400013055No Hit
GGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCCCTAATT551730.16035158828810575No Hit
CTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGG551060.16015686339703036No Hit
CACCTATTATGCCATGAATTCATAGGGAATAGGTTCCAGCAGCTCAGGCT531850.15457378107231626No Hit
CTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTTCTCTTGCGTCT528590.15362631369185983No Hit
GTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCA527310.1532543019596561No Hit
GCCTTCTTTCACTTTGCCAAACACCACATGCTTGCCATCCAACCACTCAG516360.1500718578443193No Hit
AAGCAATACAGAGTATGGTTTAGGGCCCTGGATCGTGCGAGAGAAAGAAA513750.1493133026716226No Hit
CGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGG511630.14869715824016014No Hit
CCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTTT503470.14632558344736124No Hit
GACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCCCTAATTAAGGAAC501980.1458925385403428No Hit
GTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTG501330.14570362633258307No Hit
CTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTTC497980.14473000187720608No Hit
CAGGGTCTTCACGAAGATCTGCATACCACCTCTCAGACGCAGGACCAGGT497850.14469221943565413No Hit
GGATCTTGGCCTTCACATTTTCGATGGTGTCACTGGGCTCCACTTCCAGG482810.14032108158226006No Hit
CCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAG477460.1387661887953147No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTT476400.13845811657958346No Hit
GCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATG464680.13505188415659286No Hit
GGTCTTGCCGGTCAGGGTCTTCACGAAGATCTGCATACCACCTCTCAGAC463280.134644996324495No Hit
GCCGCCGATCCACACGGAGTACTTGCGCTCAGGAGGAGCAATGATCTTGATCTTCATTGTGCTGGGTGCCAGGGC462440.13440086362523632No Hit
CCATCTTCCAGCTGCTTGCCTGCAAAGATGAGCCTCTGCTGGTCGGGAGG456470.13266577765550475No Hit
GGTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCT452630.1315497424588935No Hit
TGCGTTTTCCCAGGTCCAGTCTTGTAATGTCTGAGAGCAGTGTTCCCACC438510.12744598803802087No Hit
AGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCCCTAATTAAGGAA436930.12698678605608188No Hit
TTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGA433870.1260974455087823No Hit
CTCTGAGGTCGTAGCCGGGCTCACAGCTGTAGAACACTTCCTGCCCGGGT427560.1242635439226841No Hit
GGTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAAT423160.12298475359323371No Hit
GCTATCTAAATGGTTTTAAGCGTGGCCCGTATCTGCAGGGGCGACACGCCTGGATCGTGCGAGAGAAAGAAAACC423070.12295859651831315No Hit
GCGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGAT421440.12248486282808492No Hit
AGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTT418830.12172630765538821No Hit
CACTGGGCAGGCGGTGCCTCTAATACTGGTGATGCTAGAGGTGATGTTTT415100.12064224221701321No Hit
CACGTGTATCTCTGCTCTTGTCCAGAAGGCACCACCACAGCTGCCCACTT414880.12057830270054071No Hit
CTCTCCAGAAGGCACCACCACAGCCGCCCACTTCTGGAAGGTTCCATCCC414460.12045623635091134No Hit
CCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCAC412100.11977033971966068No Hit
GGGTCATCTTCTCGCGGTTGGCCTTGGGGTTCAGGGGGGCCTCGGTCAGC408030.11858745866491906No Hit
GGGCAGGCGGTGCCTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTAAGATTTGCCG405680.11790446837532623No Hit
GCCACCCTCACCCCCATCAGCGTTTGGATGCCTTCCTCTAGGTCCTTTAG399380.1160734731308859No Hit
GTTGGAACCTATGAACATTCTGCAGGGGCCACTGACTTCTCCACGGTGCT390900.11360889540503606No Hit
CTTTTACTTTTTTTAACCTTTCCTTATGAGCATGCCTGTGTTGGGTTGAC384880.11185927772701529No Hit
GTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG379800.11038285616483165No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC378440.10998759369936517No Hit
CGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGAC374830.10893840436088428No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAA373660.1085983623869168No Hit
GCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAG364680.10598846757817486No Hit
TTCACATTTTCGATGGTGTCACTGGGCTCCACCTCCAGAGTGATGGTCTT359410.10445682552449223No Hit
CTGGGCAGGCGGTGCCTCTAATACTGGTGATGCTAGAGGTGATGTTTTTG349110.10146329361691517No Hit
GTTGGTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGG347160.10089655699363602No Hit
AGTGAGTCCTGCAAGACTTCAGGCCCTTTCTCATCCAGCAGCTCCCTGCT347050.10086458723539975No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)