FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005284390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34407517
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA14441894.197306652496895No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCA4794041.3933118161359914No Hit
GTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGT3715121.0797408019881236No Hit
TGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTG3044670.88488512553812No Hit
GCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGG2537930.7376091683686445No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG2232750.6489134336546285No Hit
AATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATG1911990.5556896186376948No Hit
CCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTG1715230.4985044401779995No Hit
GGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACC1676940.4873760579701232No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1638660.47625058210390475No Hit
GGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAA1393960.4051324017365159No Hit
TATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAA1374660.39952316233688123No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG1332570.3872903702990251No Hit
CAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAG1288760.3745576874960201No Hit
CACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCA1127410.32766386484674265No Hit
CACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGC1111460.32302824990248497No Hit
GGTTTGTTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCT1050020.3051716867567049No Hit
ATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGC1048530.3047386418496865No Hit
TGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAAT1022870.29728096915566443No Hit
GACCATCACCCTTGAGGTGGAGCCCAGTGACACCATCGAAAATGTGAAGG977010.28395248631280195No Hit
GGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGT974460.2832113691900523No Hit
GAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATT952610.27686101266766794No Hit
CCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACT928130.2697462882892712No Hit
CCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGAGGGTTC926590.2692987116739636No Hit
GGAGAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAA875590.2544763692189704No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACAC852950.24789641170561652No Hit
ACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCA837360.24336542506104117No Hit
GTCTGACAAACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAA817970.23773002858648593No Hit
GTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTTACCA808740.23504747523629793No Hit
CACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCA783690.22776708938340423No Hit
AGAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGA772140.22441026476859696No Hit
ATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTG762150.22150682945241296No Hit
CCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTC755860.2196787405496305No Hit
AATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAAGACGAGAAGAC743810.2161765988519311No Hit
GTTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTT736890.21416541042470458No Hit
CAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAA734480.21346498208516473No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT719060.20898340324877265No Hit
ACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTT708490.20591140011643386No Hit
AGGAGAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGA699990.2034410097072683No Hit
CAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCA699190.20320850237464097No Hit
AGGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGA692130.20115662516420468No Hit
AGGAACTCGGCAAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTA675210.1962390950791363No Hit
GTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAG636300.18493051968847388No Hit
CCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCAT625030.18165507264008618No Hit
CTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAAGACGAGAAGACCCTATGG621920.18075120038449738No Hit
CCTCGCTTCGCTTTTCCTCCGCAACCATGTCTGACAAACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGA615890.17899867636481878No Hit
GTTTGTTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTT615540.17889695440679432No Hit
CAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAAGACGAGA609330.17709211623727455No Hit
AGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAA597880.1737643550390457No Hit
CATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATG591890.17202345638599845No Hit
AAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAGC570250.16573413303842877No Hit
GTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATA543020.15782016470412558No Hit
AAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGA541350.15733480564726596No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT540720.15715170612282195No Hit
CCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAG536380.1558903538433186No Hit
GTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAAGACG529820.1539837937157744No Hit
CTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGC528610.15363212637517554No Hit
GTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAAGACGAGAAGACCCTATGGAGCTTTAATTTATT510800.1484559318825593No Hit
CGTATATAAATTAGCCTAGTAACTTCCTTCCTGCCCCGACCTAGACGCAC508860.14789210160093796No Hit
GCCTTCTTTCACAGAATTATTCCAGGGTTTATGTGTCAGGGTGGTGACTTCACACGCCATAATGGCACTGGTGGC507210.14741255522739405No Hit
GGCAAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACC499440.14515432775925097No Hit
CCTTGAGGTGGAGCCCAGTGACACCATCGAAAATGTGAAGGCCAAGATCC493760.14350352569759683No Hit
AGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAGCC480810.1397398132506917No Hit
CTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGG479910.13947824250148594No Hit
CGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGG479280.1392951429770419No Hit
ACACCATCGAAAATGTGAAGGCCAAGATCCAGGATAAGGAAGGCATTCCC476960.1386208717124226No Hit
GGGTTTGTTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTC473040.13748158578254863No Hit
CACCATTGGCAATGAGCGGTTCCGCTGCCCTGAGGCACTCTTCCAGCCTT471630.13707179160879293No Hit
CGATGAAGAACGCAGCTAGCTGCGAGAATTAATGTGAATTGCAGGACACA465080.13516813782290654No Hit
CAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATA456020.13253499228090188No Hit
CACCATCGAAAATGTGAAGGCCAAGATCCAGGATAAGGAAGGCATTCCCCCCGACCAGCAGAGGCTCATCTTTGC451280.13115738633508486No Hit
GGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCG446980.12990765942221288No Hit
GGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAAAAATCCACTGCCTTCCAA445280.12941358134037978No Hit
AGCTGGAAGATGGCCGTACTCTTTCTGACTACAACATCCAGAAGGAGTCGACCCTGCACCTGGTCCTGCGTCTG443180.128803249592233No Hit
CTACTGGATGAGTTCCGCTGGGAGATGGAACATAGCACGTTTCTCTCTGG437670.12720185533876216No Hit
CAGGTATGTCAGCCACCTCCAGATGTCCTGCATGCTGAGCGTACCCAAAG426230.12387700048219115No Hit
AATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGAGGGTT425900.12378109120748236No Hit
GTCAGGGTCAATTTAGACACCACTCCCCTTATACCCCACCTCCCTTCACC424670.12342361118356782No Hit
GGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGA423870.1231911038509405No Hit
GGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAAGACG419430.12190068815485873No Hit
GGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAAGACG415080.12063642953369753No Hit
GTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAAGACGAGAAGACCCT408930.11884902941412481No Hit
CCCAAAGACACACGTGACCCACCACCCCCTCTCTGACCATGAGGCCACCC408910.11884321673080914No Hit
ATCACACTGACCTGGCAGCGGGATGGGGAGGACCAGACCCAGGACACGGA408360.11868336793962783No Hit
AGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCAT402170.11688434245342377No Hit
CTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAAAAATCCACTGCCTTCCAA401600.11671868097892679No Hit
GGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGCGTT398970.11595431312291439No Hit
GACAAACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAA397380.11549220479931754No Hit
GCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAA393600.11439360765265336No Hit
GCTCCGCATCTCCCTGCTGCTCATCGAGTCGTGGCTGGAGCCCGTGCGGT391480.11377746322119087No Hit
AACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTAT386980.11246960947516207No Hit
AATTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACT386030.1121935070176671No Hit
GAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTG385860.11214409920948379No Hit
ACAACAAGTACGTGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAGGTCCCGCAGAAGCTACAGCTGCC375950.10926391462656256No Hit
GTTAAAGCCAACAAGCACCAGATCAGACAGGCTGTGAAGAAGCTCTATGA375890.10924647657661551No Hit
CTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAG368580.10712194082473316No Hit
CCATGTCTGACAAACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCA367920.10693012227531559No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA363300.10558739242939269No Hit
CATGTCTGACAAACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAG361110.10495090360632532No Hit
GGATAAAGAAGGCATCCCTCCCGACCAGCAGAGGCTCATCTTTGCAGGCA357450.10388718255955523No Hit
ATTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTT357360.10386102548463465No Hit
CACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGCGTTGCTTGGCTGCAACTGCCGTC353450.10272464589641851No Hit
CCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAAGTAGGGACCTG353350.10269558247984009No Hit
GAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAAC350140.10176264680767286No Hit
GTTTACCAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCT348380.10125113067589271No Hit
CTTTTCCTCCGCAACCATGTCTGACAAACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGA347820.10108837554305358No Hit
CATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCT344110.10001012278799426No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)