FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005284409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12405542
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA1291591.0411395165160862No Hit
CAAAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTT872540.7033469396177934No Hit
GATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCC714390.5758635938679664No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG637830.5141492407183822No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG619530.4993977691583326No Hit
AGAACGAGAGAAGGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCACTGCCGCGTCTGACGGGACAAGGG539550.43492658361883746No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCA506890.40859964038653046No Hit
GAACGAGAGAAGGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCA499750.4028441482040849No Hit
GTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGT492650.39712089967532255No Hit
GTTTCTGATTCCCCCTGGCTTATTCTTTACTTTTTCCCACTTTTCCAGGCTCAGCAGGGAGCTGCTGGATGAGAA472250.3806766362969067No Hit
CCTCTGCGCTATGTGGACATTGCCATCCCATGCAACAACAAGGGAGCTCA457460.36875454534755514No Hit
ATCGAATAGAAGCCGTGACGTATATAAATTAGCCTAGTAACTTCCTTCCT439630.35438193671828283No Hit
AACGAGAGAAGGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCAC417820.33680108454753527No Hit
TGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTG417050.33618039421413426No Hit
GCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGG414090.33379436384157984No Hit
GCATATATTCGCGAACATTCCCAAGTTGGCAAAAGAGTCCCATGGTTTTCTTTCTCTCGCACGATCCAGGGCCCT374350.30176029390735204No Hit
AGGAGAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGA363320.29286910640421837No Hit
GCTGCAGGAGGAGGAGAGAGAAAGGAGAGACAATTATGTTCCTGAGGTCTCAGCCTTGGATCAGGAGATTATTGA354330.28562234523892627No Hit
GGAACGCCCAAGAAGGGGGGCACGGAGAAAAATGGGAGGAGCAGAAGTGAAAGAGAAAGAGAAGATGGTGAATCC327800.2642367419335649No Hit
CTGAAGTTTGCTGCTGCCACTGGAGCCACTCCAATTGCTGGCCGCTTCAC314890.25383010270732226No Hit
GGAGAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAA312610.2519922144473817No Hit
CAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAG309610.24957394042114403No Hit
GTGTCCCTGTGGGTGGACGTGGTTGGTGATTGGCAGGATCCTGGTATCCG308010.24828419427381732No Hit
AGAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGA301790.24327030612608463No Hit
CAGCGGTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCC290560.23421790035453507No Hit
CGCGGCTCGGGACAACAAGAAGACGCGCATCATCCCTCGTCACCTCCAGC271540.2188860430281885No Hit
AGAACAACCGCCGCATACGGCCGATTGTCGCAGCCCGGGTCGATTATAAC264140.21292096709680236No Hit
GCCTTTTGGGTCCTGTATCCCAATTCTCAGAGGTCCCGCCGTACGCTGAG263510.2124131295512925No Hit
TTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA261990.21118787071133208No Hit
CCTGAAACGTGTGAAGGAAAATGATCAGAAAAAGAAAGAAGCCAAAGAGA260080.20964823624796078No Hit
GGGGAAACGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCATTCA259620.20927743423060438No Hit
GGGCAACCCTACTGTGGCCGTACTGCGCCTTCCTGCACTGAAGCACCCCT259160.20890663221324793No Hit
GGGAAGATGGCGGACATTCAGACTGAGCGTGCCTACCAAAAGCAGCCGAC248670.200450734034837No Hit
ATAAAATCGCGCTACCCCGACTGGATACTAGGCCAAATTGGTGAAGAAAGCTAGGGTGATAGGAATGAGGACTAT239870.19335713022453999No Hit
AGAACACTTGCACACATGTGCTAATGTTTCTCTCACCCTTGCATCCTCATTACTCTCTTAGACGCTTGAATCATG239710.19322815560980727No Hit
GGAAGCTCCTGCCCCTCCTAAAGCTGAAGCCAAAGCGAAGGCTTTAAAGG239140.19276868354482216No Hit
GCCACAGGCGTCATACTTCCCAAGAAGCGGCCATAGCCCAGATGCGAGGTGGAAAAGTCACACTAGAGCGACACC235480.18981838923281225No Hit
AGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGCATG234910.1893589171678271No Hit
CGAATAGCTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAG229300.18483674473876271No Hit
GTTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTT228360.18407901887720826No Hit
CGAAGATTATCATAACAAAAGAACGGACTACTTAGCAACGTGAATGGGAGTGAGTATGGGGAAACTACACACAG219420.17687256227902015No Hit
GGCGGAGGAATTGGGGCAAGAGGTCCCGGGGAAACGAAACTGGAAACCGA219330.176800014058233No Hit
CAGCAATTGAAGTGAAGATGATTGACCGCACGCAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAG217270.17513946589354984No Hit
GGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACC215810.17396257253411418No Hit
ATTCTATGGAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCACACAAAAACGGA214650.1730275065773023No Hit
GTGCACACAAATGGACGCGTTGCGATGGGGAGAGTGTGTGGGCTGGACCA211560.17053668433027755No Hit
CGTCAAGCACCGAAATAATATCGTTAGCGCCTCGATCCTGGAGAATGACGATACGTTTTTACGCTCAACGCGACC207750.16746547631695574No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT206830.1667238722822429No Hit
CCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTG206230.16624021747699536No Hit
GGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAA205900.16597420733410922No Hit
CGGGACAAGGGATCAACCTTTCCCGGCAAGGCGGAGGAATTGGGGCAAGA205260.16545830887517854No Hit
GGACGATCTGGCCACTGGGCCCGGGAATGTCCTACTGGTGGAGGCCGTGGTCGTGGAATGAGAAGCCGTGGCAG204890.1651600550786092No Hit
CCCCTTCTTTTCAATTTCTCCTTCCAGTTGTCTTGAACAGCCTGACTCCT203280.1638622480178617No Hit
GGGAAACGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCATTCAT198280.15983179130746564No Hit
GCCATGGGCCGCCGCCCCGCCCGTTGTTACCGGTATTGTAAGAACAAGCCGTACCCAAAGTCTCGCTTCTGCCG197450.1591627354935399No Hit
CGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCATTCATGAAATC196290.158227669536728No Hit
GCGGTGGGGGCTGGGCTCCAGAATATGGGAAATACCTGCTACGTGAACGC196260.15820348679646565No Hit
AGCTGACGGTGACAAGGTTTCCCCCTAATCGAGACGCTGCAATAACACAG196160.15812287766225772No Hit
CTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCAC194850.15706689800413395No Hit
GCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGCAAGGCGG194750.15698628886992605No Hit
GTCTCTGGGACTATGGACGGGCACGCTCATATCAGGCTATATTTGGTCCGGGTTATTATCGTCGCGGTTACCGTA193320.15583357825075275No Hit
GAGCACTATGGTCAGTAATTCCTGGAGGAATAGGTACCAAGAAAAAAACG193100.15565623815549534No Hit
GGAAATTGAAGTTGGTGGTGGTCGGAAAGCTATCATAATCTTTGTTCCCGTTCCTCAACTGAAATCTTTCCAGAA192700.15533380161866364No Hit
GTAATACTTCAGATCAGTTAAGTAGGGCCATATGCCTCGGGAATAAGCTG192410.1551000351294607No Hit
AGGAGACATAACGATAGCGACTCTGTCTCAGGGGATCTGCATATGTTTGC191300.15420527373975276No Hit
CGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGT188260.15175475605983196No Hit
ATTTATTCAAGAGAAGGTAAAGAACACTTGCACACATGTGCTAATGTTTC188090.15161772053167852No Hit
CACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGC186460.15030379164408939No Hit
AGGAATTGGGGCAAGAGGTCCCGGGGAAACGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCATTCATGA185620.14962667491674286No Hit
AGGAAAGGGGAACGATCAGGTCCGGTTTGAGCTCAGCTGCTACTCACTGG184120.14841753790362405No Hit
AACCTTTCCCGGCAAGGCGGAGGAATTGGGGCAAGAGGTCCCGGGGAAACGAAACTGGAAACCGACCGCCGCCAT181070.14595895931028247No Hit
GAGCAAGATGGGTCACCAGCAGCTGTACTGGAGCCACCCGCGAAAATTCG178230.1436696598987775No Hit
GTAGCACAACACACCCCAACTGTAGACGCAGGTAGATGCTGACACAAATA177910.14341171066931216No Hit
GAGGAGAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGG177080.1427426548553864No Hit
AGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTG175440.1414206650543765No Hit
GGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGCTTGAAGC174600.14074354832702998No Hit
ACGAGACCACCTTCAACTCCATCATGAAGTGTGACGTGGACATCCGCAAA174380.14056620823177254No Hit
CCTCACCCTGAAGTACCCCATCGAGCACGGCATCGTCACCAACTGGGACG173570.13991327424468838No Hit
CATCGACAGACCTGGTATCGTCGTGACGAACGTGCTACTCGCTTATCGGG171100.13792222862975273No Hit
TAATACTTCAGATCAGTTAAGTAGGGCCATATGCCTCGGGAATAAGCTGACGGTGACAAGGTTTCCCCCTAATCG169980.13701940632662402No Hit
GGAAGAGGAGGAGGATAAAGAGATGGAAGAATTGCAGGAAGAAAAAGAATGTGAAAAACCACAAGGGGATGAGGA169210.13639871599322304No Hit
GTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAG168980.1362133149845448No Hit
CCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGAGGGTTC167770.13523794446062895No Hit
CTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTT167640.13513315258615868No Hit
GGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGT167120.13471398508827748No Hit
GCCGAAGATGGCGGAGGTGCAGGTCCTGGTGCTTGATGGTCGAGGCCATC165890.13372249273752007No Hit
GAATAGCTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCT162490.13098178217445075No Hit
CAGAGCTCTGGCAGCCTTCCTCAAAAAGTCCGGGAAGCTGAAAGTCCCCG162430.130933416693926No Hit
GGGGAAGGAAAAGAAGGAAGAACAGGTCATCAGCCTCGGACCTCAGGTGG159270.1283861680529557No Hit
TATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAA158900.12808791425638638No Hit
AAAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTTA157610.1270480564251042No Hit
GTGAAGATGATTGACCGCACGCAATTGATATTAGATATTTTTGCAAAGCG157440.12691102089695075No Hit
CACAAATACACGACAGAATCGGACACCTCGCGAGATGACCGTTGGCCCTGAGTATTTCTACGTCTAACGAGGGTG156500.12615329503539627No Hit
GTCAGTAATTCCTGGAGGAATAGGTACCAAGAAAAAAACGAACCTTTGGGTTCCAGAGCTGTACGGTCGCACTGA156370.126048503160926No Hit
GGACAAGGGATCAACCTTTCCCGGCAAGGCGGAGGAATTGGGGCAAGAGG153750.12393654384467846No Hit
AGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGCTTGAAGCTGATC153350.12361410730784676No Hit
TAAAACGAATAGCTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTC152570.12298535606102498No Hit
TGGGAGAAGCTTCTGCTGGCAGCTCGTGCAATTGTTGCCATTGAAAACCCTGCTGATGTCAGTGTTATATCCTCC152550.1229692342341834No Hit
CCTAATCGAGACGCTGCAATAACACAGGGGCATACAGTAACCAGGCAAGA152340.12279995505234678No Hit
CCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACT151700.12228405659341607No Hit
TGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAAT151260.12192937640290122No Hit
GTACTGACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCGCTACATA151050.12176009722106458No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACAC150430.12126032058897547No Hit
CCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATCCCCGTCCTGATT149420.12044616833347546No Hit
GAGAACGAGAGAAGGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATG148900.1200270008355943No Hit
CGGAGGAATTGGGGCAAGAGGTCCCGGGGAAACGAAACTGGAAACCGACC147360.11878562016879231No Hit
CCTGGAATGGGTGCAATGGGTGGAATGGGAGGTGGTATGGGAGGTGGCATGTTCTAACTCCTAGACTAGTGCTTT147030.11851961002590616No Hit
TATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAAACTTCAAATTGAGC145860.11757648315567348No Hit
AGATGATTGACCGCACGCAATTGATATTAGATATTTTTGCAAAGCGGGCG145110.11697191464911409No Hit
GAAACAGGTCCATCCCGACACTGGCATCTCTTCCAAGGCCATGGGCATCA144470.11645601619018338No Hit
ATTTTGAAAATTCTATGGAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCACAC143720.11585144768362397No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT142990.11526300100390616No Hit
GTAATTCCTGGAGGAATAGGTACCAAGAAAAAAACGAACCTTTGGGTTCC140860.11354602644527743No Hit
GATTATCCGAAATGTTTCATTGTGGGAGCAGACAATGTGGGCTCCAAGCA140650.11337674726344081No Hit
CGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCTTGTCGGCTA139550.11249004678715367No Hit
AGAAGATTCAAGGGCCAGATCTTGATGCCCAACATTGGTTATGGAAGCAACAAAAAAACAAAGCACATGCTGCCC139040.11207894020269328No Hit
AGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCACAC138340.11151467626323783No Hit
CCATATGCCTCGGGAATAAGCTGACGGTGACAAGGTTTCCCCCTAATCGA137390.11074888948826259No Hit
CCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTC135910.10955587430198535No Hit
TATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGC135840.10949944790803981No Hit
GTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTTACCA135470.1092011941114705No Hit
GGTTACAGCCATGCCTCCATGCCCTGCCCAAATGAACGAGAAAGGTGGGG135110.108911001228322No Hit
AGAAGGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCACTGCCGC134760.10862886925859427No Hit
AGAGAAGGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCACTGCC134590.1084918337304408No Hit
GCGAGAACGAGAGAAGGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCACTGCCGCGTCTGACGGGACA132720.10698444292075268No Hit
GGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGCTTGAAGCTGATCTC132010.10641211806787644No Hit
AACAACGGTGCAATCTCAGCTAAACCGACGCAGTTTTGCTCCTTGGATTCTGAGCCCGGGCATCGCCCCTCGTTT131900.10632344802024774No Hit
CAAGAGGTCCCGGGGAAACGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCATTCATGAAATCAACACAC131670.10613804701156951No Hit
ACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTT131130.10570275768684674No Hit
GAAAGAAAAACGGTGTGCTTCAAATTGCGCTTGTCGGCTATACAAACGCA130580.10525940744870318No Hit
CATCATGGGAGTGGACATCCGCCATAACAAGGACCGAAAGGTTCGGCGCA130450.10515461557423288No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG130240.10498533639239625No Hit
GGATCGCGTCGATCTAGGTGACTACGCACGCCTCTGGAGTAAATCGAGTG128300.10342151918876258No Hit
ATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGCAAGGC128050.10321999635324276No Hit
GAGCTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGT127230.10255900145273783No Hit
AGACGCTGCAATAACACAGGGGCATACAGTAACCAGGCAAGAGTTCAATC127050.10241390501116357No Hit
CTTCGAACGCATCGCGGGTGAGGCTTCCCGCCTGGCGCATTACAACAAGCGCTCGACCATCACCTCCAGGG126970.10234941770379721No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG125680.10130955987251505No Hit
AATAGGTACCAAGAAAAAAACGAACCTTTGGGTTCCAGAGCTGTACGGTCGCACTGAACTCGGATAGGTCTCAGA125640.10127731621883188No Hit
GCTGGATGACTACCTGAACGGCCGGGCCGTGCAGCACCGTGAGGTCCAGG124790.10059213857806454No Hit
CCAGGCCGCTCTGGACCGTCTCAAGGTGTTTGACGGCATCCCACCGCCCT124400.10027776295465365No Hit
ATTCTGAGCCCGGGCATCGCCCCTCGTTTATGAACTAGCCTATCGCAGAC124350.10023745838754969No Hit
GCTGCAAGAAGGACCTGTGTAACTTTAACGAACAGCTTAAAAATGGTGGG124120.10005205737887149No Hit
GAAGGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCACTGCCGCG124080.10001981372518831No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)