FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005284415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46794146
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT3261560.6970017147016638No Hit
CTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACT2997480.6405673051496654No Hit
GTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGTTTCGTTTCCCCGGGACCTCTTGCCCC1907360.40760654121137285No Hit
GTTTAGGGCCCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGGGAATGTTCGCGAATAT1864380.39842163162887945No Hit
GTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGT1830060.39108738088734435No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGC1683050.3596710579994344No Hit
GTCAGACAAAGGATAAACGGTGAAAATTAACAAGAAGCAATACAGAGTATGGTTTAGGGCCCTGGATCGTGCG1640480.35057376621426106No Hit
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT1624450.34714812404098583No Hit
CGTCAGACAAAGGATAAACGGTGAAAATTAACAAGAAGCAATACAGAGTATGGTTTAGGGCCCTGGATCGTGCG1271710.2717669000733553No Hit
CCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA1262240.2697431426572033No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTC1210960.2587845069338374No Hit
CACACATAACATAACAGATCCTAAATCAAGACACAGCACCCTTTAATTAT1164590.2488751477588671No Hit
GGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCT1154070.24662700330079748No Hit
CACATAACATAACAGATCCTAAATCAAGACACAGCACCCTTTAATTATAC1145250.24474215214868972No Hit
GGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGAC1136540.2428808082104971No Hit
GGGCCCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAAC1119310.23919872370360173No Hit
GTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCC1103570.23583505509428465No Hit
CCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGG1099420.23494819202384845No Hit
GGCTAATTTATATACGTCACGGCTTCTATTCGATGATCCGATATGGTAAA1072940.2292893645286314No Hit
CCAGAATTCAAGACGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCT1011110.2160761732888554No Hit
TGAAAATTAACAAGAAGCAATACAGAGTATGGTTTAGGGCCCTGGATCGT1006840.2151636659850572No Hit
GGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTC982460.20995361257367534No Hit
GTCGTCGGAAGATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACT961200.2054103092297058No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA935980.20002074618478988No Hit
GTCGGTTTAGCTGAGATTGCACCGTTGTTATAATCGACCCGGGCTGCGAC935180.1998497846290431No Hit
GTCGGAAGATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTA926940.19808888060485172No Hit
GGAAGGAATAAAACACTGTAGAGAAGCTATTCGATGTCCTACATATAGTT900730.19248775263469922No Hit
GCCTTCTCTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCG879950.1880470262241777No Hit
GTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGG855030.18272157376266682No Hit
GTGGTGTCTAAATTGACCCTGACAACTTACAGTGCGTCTAGGTCGGGGCA837550.17898606376960058No Hit
GTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCG832350.17787481365724678No Hit
GTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCTGCGTTTGTATAGCCGACAAGCGCAATTTGAAGCAC813100.1737610512220909No Hit
GGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTT801910.1713697264610834No Hit
GTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATT800020.17096582978563174No Hit
GGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAG799750.17090813026056723No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTCTAATACTGGTG798090.17055338503239273No Hit
GTTTGTATAGCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTTCTTCTTTCACGGTATCGGCTTCTATGGCG794580.1698032912065539No Hit
CTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATG789420.16870058917198744No Hit
GCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCG789200.1686535747441571No Hit
CTCTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCGTGCGG774050.16541599028220325No Hit
GGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTC763450.16315074966855897No Hit
GTCTGCGATAGGCTAGTTCATAAACGAGGGGCGATGCCCGGGCTCAGAATCCAAGGAGCAAAACTGCGTCGGTTT744340.15906690550565877No Hit
AGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGAC735320.15713931396461428No Hit
CACGCAGAGTATGGTTTAGGGCCCTGGATCGTGCGAGAGAAAGAAAACCA732280.1564896600527767No Hit
GGGACTCTTTTGCCAACTTGGGAATGTTCGCGAATATATGCTGAAGCCGT730610.15613277780515536No Hit
GCCTACAGCACCCGGTATTCCCAGGCGGTCTCCCATCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCG725950.15513692674293061No Hit
GTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTG723450.15460267188122206No Hit
GTTTCATCAATGGCACTCTCTCACAGCCAATAACTTCAACAACTTCCCTA713640.1525062558038777No Hit
CGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATT712940.1523566644425993No Hit
GTCTAGGTCGGGGCAGGAAGGAAGTTACTAGGCTAATTTATATACGTCAC712060.15216860673127788No Hit
CACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACA709470.15161511869454783No Hit
ATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCATGA696250.148789978985833No Hit
CTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATAT676980.14467194251178342No Hit
GTCCGGTGCACCGATTGGCTGTTTTAGCTCATTGTGACGTGAGTTGATAC666430.14241738699537332No Hit
GGTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCT666190.1423660985286493No Hit
AAACAAACAAACAAACAAACAAACAAACAAACAAACAAACAAACAAACAA653170.13958369920887112No Hit
ATCGGGTCCAGCGTGGCAAACAGGAGGTCTTCTTCATAGCTGTCAGCACT646610.13818181445174788No Hit
GCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGT642990.13740821341199388No Hit
CCCTGCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTTCTTCTTTCACGGTATCGGCTTCT640860.1369530282698182No Hit
GTCACTGGGCAGGCGGTGCCTCTAATACTGGTGATGCTAGAGGTGATGTT630820.13480746074519662No Hit
GTGCCATTCGGACCTACCGTAAGCCTATATTTCGTTTTTCTGAGACCTAT629160.13445271551702215No Hit
GGCGACACGCCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGGGAATGTTCGCGAATAT619580.13240545088695496No Hit
ACACATAACATAACAGATCCTAAATCAAGACACAGCACCCTTTAATTATACAGACGATGGACAACAATAAAGTGT617820.13202933546431214No Hit
CGTCAAAGCCAGGCTATCTAAATGGTTTTAAGCGTGGCCCGTATCTGCAGGGGCGACACGCCTGGATCGTGCG609720.13029834971237642No Hit
GTAGCACCATTATCATCATAAGTAAACAAAAGGCGTCAGACAAAGGATAAACGGTGAAAATTAACAAGAAGCAAT607810.1298901789980311No Hit
GTTCCTTTTACTTTTTTTAACCTTTCCTTATGAGCATGCCTGTGTTGGGT606910.129697847247816No Hit
CTTGGGAATGTTCGCGAATATATGCTGAAGCCGTGTCTCTGTGGACGGGA603460.1289605755386582No Hit
GCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGG600270.1282788663351181No Hit
GGCGAATCATAGGGAGGTGAAGGGAGGTGGGGTATAAGGGGAGTGGTGTC594980.12714838304774276No Hit
CTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA592570.1266333613610557No Hit
GGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTG592220.1265585656804165No Hit
GTCTTCTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATT587970.125650332415512No Hit
GTTAATTGGGAAATGAAGCAATAATGATGTGGCTTATGCTTACTGGTAAA581120.12418647409443054No Hit
GATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGGGAAT580320.1240155125386838No Hit
CAGCAATCAATGTCGTCGGAAGATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGC572610.1223678705451746No Hit
GTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG564870.12071381749332491No Hit
CATTACCAGCCTAAAATTGCCAAATATGATATAGGATGACATTCCTAAAA563560.12043386794578963No Hit
GTCATCGGGTCCAGCGTGGCAAACAGGAGGTCTTCTTCATAGCTGTCAGC561850.12006843762038097No Hit
GGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATT557710.11918371156939161No Hit
CCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAAT555830.11878195191338678No Hit
GTGATGATTTGGTAAGAGATAATTTAATAGTACGAGGGCGTTCATGATTC555200.11864731968823622No Hit
CGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGG553980.11838660331572244No Hit
CTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGG552580.11808742059316564No Hit
CTGTAAATAAATAGGAATGAGAATTAAACAGTGGAAGGAATAAAACACTG552130.1179912547180581No Hit
GCCGTGTCTCTGTGGACGGGAAAGGGCGAATCATAGGGAGGTGAAGGGAGGTGGGGTATAAGGGGAGTGGTGTCT540200.11544179051798488No Hit
CCCTCACATTATTGCTCAGCGTTCTTGCAGGGGCTAATCTTTGAGGCAGT534500.11422368943328938No Hit
CAGGGGCTAATCTTTGAGGCAGTCATGTCCACACAGATCGCGAAAGATTA532050.11370011966881498No Hit
GGTGAACCGTCAAAGCCAGGCTATCTAAATGGTTTTAAGCGTGGCCCGTA529490.11315304269042543No Hit
CTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTA528010.11283676381229396No Hit
AGAATAACGAAGATTCGGCAGGGTAGAGAGTATGTTAATTGGGAAATGAA526860.11259100657590802No Hit
GGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTG525370.11227259067832973No Hit
ATCGCGAATATATGCTGAAGCCGTGTCTCTGTGGACGGGAAAGGGCGAAT521590.11146479732742638No Hit
GTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTC517010.11048604242077631No Hit
GCCAACTTGGGAATGTTCGCGAATATATGCTGAAGCCGTGTCTCTGTGGA514840.1100223092008133No Hit
CATGGGACTCTTTTGCCAACTTGGGAATGTTCGCGAATATATGCTGAAGC509230.10882344129113927No Hit
GCTCAGCGTTCTTGCAGGGGCTAATCTTTGAGGCAGTCATGTCCACACAGATCGCGAAAGATTAAGACGAGGG504240.107757068587169No Hit
CAAAGGACGGAAATACAAAAAGAGCCCAATAGACACAAAATTTGTACACGAAGCGCTTAGAACACGCGTAGAGTC504130.10773356137325384No Hit
CGCGAATATATGCTGAAGCCGTGTCTCTGTGGACGGGAAAGGGCGAATCA503790.10766090271206147No Hit
CCGTGTCTCTGTGGACGGGAAAGGGCGAATCATAGGGAGGTGAAGGGAGGTGGGGTATAAGGGGAGTGGTGTCTA501880.10725273199771612No Hit
GGCGCAAGTTGTAGGTCGGGGGCACCACACCACGCTCGCTTGGCAGTAAC500780.10701765985856437No Hit
GCCATTCGGACCTACCGTAAGCCTATATTTCGTTTTTCTGAGACCTATCC498700.10657315981362284No Hit
GCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG498170.10645989778294064No Hit
GTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATA493440.10544908758458804No Hit
GCTATCTAAATGGTTTTAAGCGTGGCCCGTATCTGCAGGGGCGACACGCA491310.10499390244241234No Hit
ATACAGAGTATGGTTTAGGGCCCTGGATCGTGCGAGAGAAAGAAAACCAT490860.1048977365673048No Hit
GTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTT487020.10407712109972048No Hit
GTGTCTCTGTGGACGGGAAAGGGCGAATCATAGGGAGGTGAAGGGAGGTG485940.10384632299946236No Hit
GGCAGGAAGGAAGTTACTAGGCTAATTTATATACGTCACGGCTTCTATTC483310.10328428688494495No Hit
CCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTTT482870.10319025802928425No Hit
CCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATA474920.10149132756905105No Hit
CTAATCTTTGAGGCAGTCATGTCCACACAGATCGCGAAAGATTAAGACGA473810.10125411841045244No Hit
CATATAGCATATAAAAATACTTAGTAGTGTGATGATTTGGTAAGAGATAA470270.10049761352627315No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)