FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005284416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46794146
Sequences flagged as poor quality0
Sequence length35-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTT10246052.189600810323582No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA4986591.0656439803389084No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCA3952850.844731732041867No Hit
GCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGG2654200.5672077015787402No Hit
GATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCC2529700.5406018094656541No Hit
AAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCC2371450.5067834767195025No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2094050.44750255726432103No Hit
GTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGT1843170.39388901338214394No Hit
AGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCT1585710.33886931070395No Hit
TGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTG1419190.30328366287526654No Hit
GTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTTAATTCCCCTATGGTCCTACTTTACAGTCTGAATAAATTTT1369330.29262848391335106No Hit
GGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGT1294620.2766628116260525No Hit
ATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGT1293310.27638286207851726No Hit
CCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTTAATTCCCCTATGGTCCTACTTTACAGTCTGAAT1278470.2732115252194153No Hit
GAACGAGAGAAGGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCA1246680.26641794039792926No Hit
CTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCAC1207720.25809211263306314No Hit
CAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAG1146130.24493020986001113No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT1079680.23072971563579767No Hit
AAAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTTA1037090.22162814981173073No Hit
CTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTT1030480.22021557995737331No Hit
AAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTTAATTCCCCT1007160.2152320506073559No Hit
CATGAATCATACCGATTAATTCTGTGCTACTCTTCGCTTCCTCGAACACC982120.209880953912483No Hit
CTTTACAGTCTGAATAAATTTTACCATATCGGATCATCGAATAGAAGCCG951820.20340578498857526No Hit
CACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCA921520.19693061606466758No Hit
GTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAG916690.19589843567184664No Hit
CCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTC891190.19044903608241937No Hit
GGAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCA877400.18750208626523499No Hit
CGTATATAAATTAGCCTAGTAACTTCCTTCCTGCCCCGACCTAGACGCAC871550.18625192988883696No Hit
CAATAACGTCTCTTTCAGAAGGCATTGGTATCTTTTCCCCACTTCCAAGC870480.1860232688080257No Hit
CAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTTAATTCCCCTATGGTCCTACTTTACAGTCTGAATAA856650.18306777091305396No Hit
GTTTGATTGTTCGGATAATTATGTTTCACTATGCTCCTCCGTTCCCATCT854900.18269379250985796No Hit
GGATCATCGAATAGAAGCCGTGACGTATATAAATTAGCCTAGTAACTTCC820950.17543861148785578No Hit
AGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCACAC795460.16999134891787532No Hit
GGTCAATTTAGACACCACTCCCCTTATACCCCACCTCCCTTCACCTCCCTATGATTCGCCCTTTCCCGTCCAC772720.16513176669577428No Hit
GCTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCA770160.16458468971738474No Hit
ATTGGGGCAAGAGGTCCCGGGGAAACGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCATTCATGAAATC759620.16233227122042146No Hit
GTTTCTCTCACCCTTGCATCCTCATTACTCTCTTAGACGCTTGAATCATG756910.1617531389503294No Hit
GTCAATTTAGACACCACTCCCCTTATACCCCACCTCCCTTCACCTCCCTATGATTCGCCCTTTCCCGTCCACAG749440.16015678542354422No Hit
TAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCAT715670.15294007075158503No Hit
CCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTG713740.15252762599834604No Hit
CCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACT711590.15206816681727667No Hit
AATGAACCTGCCGGCTGATCGTTTTTAGGATATTGTGAGTAATATAGATT707390.1511706186496063No Hit
GGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACC675860.14443259633373798No Hit
CACCTCCCTTCACCTCCCTATGATTCGCCCTTTCCCGTCCACAGAGACAC674730.14419111313624572No Hit
CCTCCCTTCACCTCCCTATGATTCGCCCTTTCCCGTCCACAGAGACACGG672080.14362480298283464No Hit
GTCCTACTTTACAGTCTGAATAAATTTTACCATATCGGATCATCGAATAG659340.14090224020756786No Hit
GGGGAAACGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCATTCA658350.14069067528233126No Hit
ACCCCACCTCCCTTCACCTCCCTATGATTCGCCCTTTCCCGTCCACAGAGACACGGCTTCAGCATATATTCGCGA652770.13949821843099774No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT639880.13674360036402844No Hit
AGAACGAGAGAAGGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCACTGCCGCGTCTGACGGGACAAGGG638010.13634397772747045No Hit
ATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCA613860.13118307576336577No Hit
ATTTTGTGTCTATTGGGCTCTTTTTGTATTTCCGTCCTTTGCTGTTTACC610270.1304158857819523No Hit
GTACACTTTATTGTTGTCCATCGTCTGTATAATTAAAGGGTGCTGTGTCT609580.13026843144012074No Hit
AGCCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTTAATTCCCCTATGGTCCTACTTTACAGTCTGA602000.1286485706994204No Hit
ATTCAAGAGAAGGTAAAGAACACTTGCACACATGTGCTAATGTTTCTCTCACCCTTGCATCCTCATTACTCTCTT599920.12820407065447886No Hit
GTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTTACCA591760.12646026278586214No Hit
AATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATG590520.1261952723744547No Hit
CTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGG588730.12581274589347138No Hit
CTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAAGACGAGAAGACCCTATGG574950.12286793309573381No Hit
ACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTT569080.12161350268044212No Hit
GTTTACCATTGGTCTGTTTTAGGTAGTATCTTCCATTCAGTATTTTATTCGGAAGATGTTAAAAGGAGCAGAAAT566440.12104932954647789No Hit
AAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCA563230.1203633463040441No Hit
AAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTTC554360.11846781005470214No Hit
AATTGGGGCAAGAGGTCCCGGGGAAACGAAACTGGAAACCGACCGCCGCC552980.11817290137103902No Hit
CGATGAAGAACGCAGCTAGCTGCGAGAATTAATGTGAATTGCAGGACACA551290.11781174508452402No Hit
TGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAA550590.11766215372324564No Hit
TATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAA543470.11614059587709967No Hit
CACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGC540480.11550162706249624No Hit
GGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCACTGCCGCGTCT535590.1144566245529943No Hit
AGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGCTTGAAGCTGATC533070.11391809565239207No Hit
CTCCCTTCACCTCCCTATGATTCGCCCTTTCCCGTCCACAGAGACACGGC529470.11314876865153176No Hit
TGAATATTAATGTTTCCCGGTTTTAATGTGTGATTTTTTGAGCTGTGGTC525970.11240081184513977No Hit
GAGATGCCTTCCATGGTTTCCTCCCTTCCTTAATTCCCCTATGGTCCTAC525720.11234738635896892No Hit
CGAGAGAAGGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCA521190.111379316549553No Hit
CCATGGTTTCCTCCCTTCCTTAATTCCCCTATGGTCCTACTTTACAGTCT518800.11086856890175964No Hit
GTTTGTTTGTTTGTTTGTTTGTTTGTTTGTTTGTTTGTTTGTTTGTTTGT511310.10926794133608081No Hit
AAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCACACAAAAACGGAACAGGGCTG510150.10902004708024803No Hit
GTCCATCGTCTGTATAATTAAAGGGTGCTGTGTCTTGATTTAGGATCTGTTATGTTATGTGTGTTACTTTTATGA507770.10851143645190149No Hit
ACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCA500420.10694072715847831No Hit
CAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAAGACGAGA498580.10654751558026082No Hit
CCCTCCCCTTATACCCCACCTCCCTTCACCTCCCTATGATTCGCCCTTTCCCGTCCACAGAGACACGGCTTCAGC496580.10612011169089398No Hit
GAAGGTTTTCTCAAACTTCACTAGTGCTGTGTTATTAACATTAATTATTGTCTTATTCTATTCTCTTGGATGAAT494790.10573758520991065No Hit
AGATGATTGACCGCACGCAATTGATATTAGATATTTTTGCAAAGCGGGCG492840.10532086641777798No Hit
GTAAGTTGTCAGGGTCAATTTAGACACCACTCCCCTTATACCCCACCTCC490860.1048977365673048No Hit
GTATAATTAAAGGGTGCTGTGTCTTGATTTAGGATCTGTTATGTTATGTGTGTTACTTTTATGAAGTTAAAATT490010.10471608991432389No Hit
CTTCCATTCAGTATTTTATTCGGAAGATGTTAAAAGGAGCAGAAATAGCG481320.10285902001502495No Hit
AAAACGAATAGCTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCC480490.10268164740093771No Hit
AAGGGATCAACCTTTCCCGGCAAGGCGGAGGAATTGGGGCAAGAGGTCCC472840.10104682752410954No Hit
AGGAATTGGGGCAAGAGGTCCCGGGGAAACGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCATTCATGA472430.10095920972678933No Hit
GAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATT470620.10057240920691234No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)