FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005284419

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284419
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40090202
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGGGAATGTTCGCGAATATATGCTGA2479450.6184678241331885No Hit
CTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACT1888830.47114504436769866No Hit
GGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTC1771260.4418186767928982No Hit
GTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGG1557610.38852635364621013No Hit
GTCTAAATTGACCCTGACAACTTACAGTGCGTCTAGGTCGGGGCAGGAAG1550380.3867229204781757No Hit
CCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGG1464770.36536857559360764No Hit
GTCCTACATATAGTTATAGAATAACGAAGATTCGGCAGGGTAGAGAGTAT1424790.35539606410563856No Hit
GTATGGTTTAGGGCCCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGGGAATGTTCGCG1409250.35151980526314136No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1357560.338626380580472No Hit
CGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATT1290490.3218966070562578No Hit
ATAACATAACAGATCCTAAATCAAGACACAGCACCCTTTAATTATACAGACGATGGACAACAATAAAGTGTAC1277640.318691335104772No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT1242730.3099834717719806No Hit
GTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGTTTCGTTTCCCCGGGACCTCTTGCCCC1179140.29412174076848No Hit
GTGATGATTTGGTAAGAGATAATTTAATAGTACGAGGGCGTTCATGATTC1151470.28721980497878263No Hit
GTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGT1097130.27366537090533993No Hit
GGAGCGGTGAACCGTCAAAGCCAGGCTATCTAAATGGTTTTAAGCGTGGC1060320.26448357631123937No Hit
AGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGGGAATGTTCGCGAA1058890.2641268806777277No Hit
GCACAAGCAGGTAGATCATCACTCTAAGGACACCACCCTCATCCACCAAATATTTGGAGGCTACTGGAGATCTCA1055140.2631914900304069No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGC958490.23908335508012654No Hit
CGAGTCAAGAAATAGCGTAGCACCATTATCATCATAAGTAAACAAAAGGCGTCAGACAAAGGATAAACGGTGAAA926670.23114625364072747No Hit
CATAGAAAGGTACTCACCTCCCACGTCGAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTTCC917660.22889882171209813No Hit
GGAAAGACAGCGATCCTTAATTCGGAGCCAAAAAGATCTCGTAGGCTTTATCTGGGACTGGGACACAGTTACTTT914020.22799086919043213No Hit
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT907580.22638449165209995No Hit
AGGGGCGACACGCAGAGTATGGTTTAGGGCCCTGGATCGTGCGAGAGAAA872050.21752197706561816No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT860860.214730771374013TruSeq Adapter, Index 27 (97% over 39bp)
CTCATGTTCACGTAGCCTCTCCTCCTGTTCACACAGCCTCTCCTCCTGTT848080.2115429600479439No Hit
GGGGCGACACGCAGAGTATGGTTTAGGGCCCTGGATCGTGCGAGAGAAAG847160.21131347754246785No Hit
GGCTAATTTATATACGTCACGGCTTCTATTCGATGATCCGATATGGTAAA845920.21100417503508712No Hit
GGAGAGGCTACGTGAACAGGAGGAGAGGCTGTGTGAACAGGAGGAGAGGC843850.21048783939776605No Hit
GGATCGTATAACCCATCAGATTTAGCGCCACGTCCGCTCGTTCCGAAAACTCCACCGGGCCATTTTACTTTTTA819670.20445644050384182No Hit
GGCTCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGGGAATGTTCGCGAATATATGCTG818320.20411969987080633No Hit
AGGGCCCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAA810830.20225141295122434No Hit
GTCTCTGTGGACGGGAAAGGGCGAATCATAGGGAGGTGAAGGGAGGTGGG809480.20191467231818888No Hit
GTGAAGTTTGAGAAAACCTTCTAAAACACACCTGGATCGTGCGAGAGAAA800200.1995998922629524No Hit
GTCGGAAGATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTA797390.1988989728712267No Hit
CCCCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGGGAATGTTCGCGAATATATGCTGA787700.1964819234385499No Hit
TGGGACTCTTTTGCCAACTTGGGAATGTTCGCGAATATATGCTGAAGCCGTGTCTCTGTGGACGGGAAAGGGCGA766450.1911813764370656No Hit
GCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGGGAATGTTCGC764540.19070495080069688No Hit
GTCAAAGCCAGGCTATCTAAATGGTTTTAAGCGTGGCCCGTATCTGCAGG758670.18924075264075746No Hit
GGGCCCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAAC749600.18697835446177097No Hit
GCATCTTCTCTTCCTGCTCACACATCTTCTCCTCCTGCTCCCACATCTTC741830.1850402250405224No Hit
GGAATGAGAATTAAACAGTGGAAGGAATAAAACACTGTAGAGAAGCTATT729510.18196715496719124No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG720990.1798419474164785No Hit
GCCCAATAGACACAAAATTTGTACACGAAGCGCTTAGAACACGCGTAGAGTCATTTAGAATTAATCGAGTCAAGA720700.17976961053975232No Hit
ATTAAACAGTGGAAGGAATAAAACACTGTAGAGAAGCTATTCGATGTCCTACATATAGTTATAGAATAACGA711780.17754462798665868No Hit
AGGGCGTTCATGATTCAAGCGTCTAAGAGAGTAATGAGGATGCAAGGGTGAGAGAAACATTAGCACATGTGTGCA701420.17496045542499386No Hit
GTCAAGAAATAGCGTAGCACCATTATCATCATAAGTAAACAAAAGGCGTCAGACAAAGGATAAACGGTGAAAATT700130.17463868104231553No Hit
CTATCTAAATGGTTTTAAGCGTGGCCCGTATCTGCAGGGGCGACACGCCT696940.173842975398328No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA692120.17264068661963838No Hit
GTCGGGGCAGGAAGGAAGTTACTAGGCTAATTTATATACGTCACGGCTTC665740.1660605252126193No Hit
GTTAATTGGGAAATGAAGCAATAATGATGTGGCTTATGCTTACTGGTAAA656230.16368837453101384No Hit
GGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGAC650490.16225660324684818No Hit
GGGCTCTTTTGCCAACTTGGGAATGTTCGCGAATATATGCTGAAGCCGTG634790.15834043440339862No Hit
GACGTCTTTGGCTGCCTTCCCCTATGTTTCTACGCTCTGCGGACTTACTC632640.15780414376560137No Hit
CTGGCTGTATCGATCAGTCCATGTTGCATTACCAGCCTAAAATTGCCAAATATGATATAGGATGACATTCCTAAA628750.15683383186744732No Hit
GCGTTAGAGTAGAAGTGTGCTGTTGATGTCAGCCTCCTTAAACCGATAGT628540.15678144999119734No Hit
CGAGCGTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGA622480.155269858705127No Hit
CGTTCATGATTCAAGCGTCTAAGAGAGTAATGAGGATGCAAGGGTGAGAG622050.15516260057756753No Hit
GGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAG616970.15389545804733037No Hit
GTCACGGCTTCTATTCGATGATCCGATATGGTAAAATTTATTCAGACTGT616350.15374080679364No Hit
GAAGCCGTGTCTCTGTGGACGGGAAAGGGCGAATCATAGGGAGGTGAAGG614880.15337413365989025No Hit
CCCGTGTTTTCATTTCAGCACCGACTGAGCAAAGGCCTGGGGTGCAGGAGTGCTACCATGGTAATGGACAGAGTT607940.15164303736858198No Hit
AAAGAAAACCATGGGACTCTTTTGCCAACTTGGGAATGTTCGCGAATATA607300.15148339736477257No Hit
GTGGCTTATGCTTACTGGTAAAAAGACTTTGTAATGAGTAAAAAATTCATAACAGAGAGTGACCATATAGCATAT600750.14984958170078563No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTC598860.14937814481453598No Hit
GCTGAAGCCGTGTCTCTGTGGACGGGAAAGGGCGAATCATAGGGAGGTGA595850.14862733792161986No Hit
GTCGTCGGAAGATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACT586490.14629260286590723No Hit
GGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCG584810.14587354785590756No Hit
GTGGGGTATAAGGGGAGTGGTGTCTAAATTGACCCTGACAACTTACAGTG581150.1449606065841225No Hit
GTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAG574430.14328438654412368No Hit
CATGGTTTAGGGCCCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGGGAATGTTCGCGA570620.1423340296464458No Hit
GGGGAGGAACATATCAGTCGGACGGTCCCTGCAACCCGTTATACGACCCA570500.14230409714573153No Hit
ACATAACATAACAGATCCTAAATCAAGACACAGCACCCTTTAATTATACAGACGATGGACAACAATAAAGTGTAC568270.1417478515074581No Hit
CTATAATGGTGCTGGGCGTGTGTTGAACCCAATGACGGGTCGCAGCGTCA564820.14088729211192302No Hit
GTCTAGGTCGGGGCAGGAAGGAAGTTACTAGGCTAATTTATATACGTCAC558540.13932082457454317No Hit
GGGAACAATTAGCGCCAAAGAAACACAGACCAGGATAAGGAAGATGGGAACGGAGGAGCATAGTGAAACATAATT558270.1392534764479361No Hit
TTTAGAATTAATCGAGTCAAGAAATAGCGTAGCACCATTATCATCATAAG557320.1390165108172815No Hit
GTCCGGTGCACCGATTGGCTGTTTTAGCTCATTGTGACGTGAGTTGATAC556360.13877705081156735No Hit
CTAGTTAAGCTGTCAACCGGTCACTTACCTCGAGGGTGCTAGCAGAAGAACCCGCATAGGCCACAGTAGTATCGA553710.13811604142079403No Hit
CAGCGTTCTTGCAGGGGCTAATCTTTGAGGCAGTCATGTCCACACAGATC543190.13549195885817686No Hit
ATCTAAATGGTTTTAAGCGTGGCCCGTATCTGCAGGGGCGACACGCAGAG542960.13543458823180787No Hit
GGGGAGTGGTGTCTAAATTGACCCTGACAACTTACAGTGCGTCTAGGTCGGGGCAGGAAGGAAGTTACTAGGCTA538800.1343969282070467No Hit
CCTTAACTTATTTCTGTGAGCGTTAGAGTAGAAGTGTGCTGTTGATGTCA538610.1343495350809158No Hit
GTCTAAGAGAGTAATGAGGATGCAAGGGTGAGAGAAACATTAGCACATGT537260.1340127944478803No Hit
CTTTAATTATACAGACGATGGACAACAATAAAGTGTACAGACCACAGCTCAAAAAATCACACATTAAAACCGGGA536490.1338207275682971No Hit
AAGTCAGGCAGTTCAAGATAACCTGAAGGAGTCGAATAACATCTATCCAG533910.13317717880294044No Hit
GGACGGGAAAGGGCGAATCATAGGGAGGTGAAGGGAGGTGGGGTATAAGG533170.1329925950485358No Hit
GGGAAAGACACAGTGAGAGTGTGTCAAGAGGCAGGGAACCAAGAGCCCTT532800.1329003031713335No Hit
GTCATGTCCACACAGATCGCGAAAGATTAAGACGAGGGAGATTCTGGCTG524120.13073518561966838No Hit
CCAGAATTCAAGACGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCT523650.13061794999187085No Hit
CACAACGATTGGGAACATGACTTAGCAACAGATTATTCAGATGACCCTAA519200.12950795309038354No Hit
CCTGGAAGCTGCCAGCCAGCAGAAACAGCAGCTAACGGCCCAGCTGAGCC515800.12865986557014605No Hit
CGAGGGCGTTCATGATTCAAGCGTCTAAGAGAGTAATGAGGATGCAAGGG514390.12830815868675344No Hit
GGTCCAGCGTGGCAAACAGGAGGTCTTCTTCATAGCTGTCAGCACTCGTC505810.12616798488568354No Hit
CAAAAAGAGCCCAATAGACACAAAATTTGTACACGAAGCGCTTAGAACACGCGTAGAGTCATTTAGAATTAATCG499490.12459153984806562No Hit
GTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCTGCGTTTGTATAGCCGACAAGCGCAATTTGAAGCAC495320.12355138544824494No Hit
TGTCATTGTTGAGGAGCATCCACTTCTCAATACGAGGAAATAAGACATCACCCAGAGCACATTTTTCAGTTCTGG491740.12265839917693605No Hit
ACACATAACATAACAGATCCTAAATCAAGACACAGCACCCTTTAATTATACAGACGATGGACAACAATAAAGTGT490500.12234909666955533No Hit
GATTCGGCAGGGTAGAGAGTATGTTAATTGGGAAATGAAGCAATAATGATGTGGCTTATGCTTACTGGTAAAA490310.1223017035434244No Hit
GTCTGCGATAGGCTAGTTCATAAACGAGGGGCGATGCCCGGGCTCAGAATCCAAGGAGCAAAACTGCGTCGGTTT486820.1214311666476512No Hit
GGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGGGAA482540.12036357412217578No Hit
ATCCTAAATCAAGACACAGCACCCTTTAATTATACAGACGATGGACAACAATAAAGTGTACAGACCACAGCTCAA481290.12005177723973554No Hit
AGACTGTAAAGTAGGACCATAGGGGAATTAAGGAAGGGAGGAAACCATGG480440.11983975535967617No Hit
CCGTTATACGACCCATCCTAAGTATCGCCTTCATACGGAGCCACTTCGAA479880.11970007035634292No Hit
GCTTAGCGTTCTTGCAGGGGCTAATCTTTGAGGCAGTCATGTCCACACAGATCGCGAAAGATTAAGACGAGGG475170.11852521970330805No Hit
GGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTT473930.11821591719592733No Hit
GGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTG472590.11788167093795138No Hit
GTCCGATTCTGTCGTGTATTTGTGTCTCCTGCTGCACTGAATATGGCATT467740.11667189903408319No Hit
CCTAACATTATTGCTCAGCGTTCTTGCAGGGGCTAATCTTTGAGGCAGTCATGTCCACACAGATCGCGAAAGATT466130.11627030464950014No Hit
CAGAGTATGGTTTAGGGCCCTGGATCGTGCGAGAGAAAGAAAACCATGGG466010.11624037214878588No Hit
GGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCT460220.11479612898932262No Hit
CCGGGAAACATTAATATTCATCCAAGAGAATAGAATAAGACAATAATTAA458720.11442197273039434No Hit
GTTGGTTAAATCTTCTATCATCCCTGTCCTGCCTGGCTCATCAGGAATCT448940.11198247392218179No Hit
CTGTTGATGCACGAGAGGTTGGCCAAGTGTCTGTAAATAGCAACAAAGAAAAAAGACAGAATTAGACTTGAAGGG448890.11197000204688416No Hit
GGCCCCGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGGGAATGTTCGCGAATATATGCTG446770.11144119453426551No Hit
AGGGAGGTGGGGTATAAGGGGAGTGGTGTCTAAATTGACCCTGACAACTTACAGTGCGTCTAGGTCGGGGCAGGA438660.10941825636099314No Hit
GTAAATAAATAGGAATGAGAATTAAACAGTGGAAGGAATAAAACACTGTA436970.10899670697593392No Hit
CATGCTGAAGCCGTGTCTCTGTGGACGGGAAAGGGCGAATCATAGGGAGGTGAAGGGAGGTGGGGTATAAGGGG429570.10715086943188762No Hit
GCAGTAATAGAGAGATTCAAGACGCGTATGGGCCAACCATGGTGAGATTT427590.10665698317010226No Hit
CTTACTGGTAAAAAGACTTTGTAATGAGTAAAAAATTCATAACAGAGAGTGACCATATAGCATATAAAAATACTT422420.10536739126432938No Hit
GAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGGGAATGTTCGCGA421220.10506806625718675No Hit
GACGGAAATACAAAAAGAGCCCAATAGACACAAAATTTGTACACGAAGCGCTTAGAACACGCGTAGAGTCATTT418630.10442202311677053No Hit
GGCGACAGAGGAGAGAATGAATGGACAGGCTTATAAGCATTAAAAGCTAG414610.10341928434284267No Hit
GTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCC411700.10269342120052176No Hit
CCCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGGGAATGTTCGCGAATATATGCTGA411570.10266099432474798No Hit
TATGTTAATTGGGAAATGAAGCAATAATGATGGGGCTTATGCTTACTGGTAAAAAGACTTTGTAATGAGTAAAAA402730.1004559667721305No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)