FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005284420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40090202
Sequences flagged as poor quality0
Sequence length35-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTT12309393.070423541392982No Hit
GATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCC3639340.9077878929120885No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2352530.5868092158777349No Hit
ATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGT2294740.5723942224087571No Hit
AAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCC2048830.5110550453200511No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA2006700.5005462431942848No Hit
ACCCCACCTCCCTTCACCTCCCTATGATTCGCCCTTTCCCGTCCACAGAGACACGGCTTCAGCATATATTCGCGA1244240.3103601224059684No Hit
CGTATATAAATTAGCCTAGTAACTTCCTTCCTGCCCCGACCTAGACGCAC1192250.2973918664715134No Hit
GTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGT1174100.29286457573848096No Hit
AGGGCGATGTCCAGGTAAGGGTCAAAAGTGTCTGAAATGCCGTGGCAGTG1054010.2629096256486809No Hit
CCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTTAATTCCCCTATGGTCCTACTTTACAGTCTGAAT1050660.26207401000374103No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCA1009870.2518994541359507No Hit
AAAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTTA980680.24461837333720596No Hit
CACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGAC921330.22981425735894273No Hit
AATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTTCC859910.2144938057433584No Hit
AAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTTC838720.20920822499223127No Hit
CACATGAATCATACCGATTAATTCTGTGCTACTCTTCGCTTCCTCGAACA834540.20816557621735104No Hit
CTCCTGTTCACGTAGCCTCTCCTCCTGTTCACACAGCCTCTCCTCCTGTT819480.20440904737771087No Hit
AATAGTCTGAAGTCGGTGTCAGCCCTTCTCTCTTCTAGATAGCCAACCATTGCTATTCATATCTTGGTCTTAT814650.20320426422396173No Hit
GAACATGAGGAGAGGCTGTGTGAACAGGAGGAGAGGCTATGTGAACAGGAGGAGAGGCTACGTGAACAGGAGG778230.19411975025718256No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT768270.1916353526978986No Hit
ATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTTCCT757420.1889289557583172No Hit
GATGGGGGAGCAGGAAGAGAAGATGCAAGAACAGGAGGAGAAGATGCGGAGGCAGGAGGAGAAGATAAGGGAGC732330.18267056873397644No Hit
GCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGG729810.1820419862189769No Hit
GTTTACCATTGGTCTGTTTTAGGTAGTATCTTCCATTCAGTATTTTATTCGGAAGATGTTAAAAGGAGCAGAAAT725260.18090704556689438No Hit
CCTAGACGCACTGTAAGTTGTCAGGGTCAATTTAGACACCACTCCCCTTA717570.17898887114612194No Hit
TAAATTTGAAAATCATCACCACCATCCTTAGGAATTTATTCAAGAGAAGGTAAAGAACACTTGCACACATGTGCT714580.17824305300332485No Hit
GTACACTTTATTGTTGTCCATCGTCTGTATAATTAAAGGGTGCTGTGTCT688410.17171527347255572No Hit
TCCCAAGTTGGCAAAAGAGTCCCATGGTTTTCTTTCTCTCGCACGATCCA685390.17096197220458006No Hit
TTAATGTTTCCCGGTTTTAATGTGTGATTTTTTGAGCTGTGGTCTGTACACTTTATTGTTGTCCATCGTCTGTAT680150.16965491967339053No Hit
CCCAAGTTGGCAAAAGAGTCCCATGGTTTTCTTTCTCTCGCACGATCCAG670950.16736009461863027No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG648000.1616355038570272No Hit
CTCTCCTCCTGTTCACGTAGCCTCTCCTCCTGTTCACACAGCCTCTCCTC634860.15835789502881525No Hit
GTCAATTTAGACACCACTCCCCTTATACCCCACCTCCCTTCACCTCCCTATGATTCGCCCTTTCCCGTCCACAG619710.1545789168136394No Hit
ATTCAAGTTTGTCCTTTTTCATTTACTTTAGCCTGAGCATACACATTTTC619650.15456395056328226No Hit
CGTCAAGCACCGAAATAATATCGTTAGCGCCTCGATCCTGGAGAATGACGATACGTTTTTACGCTCAACGCGACC619400.1545015911867942No Hit
GGCAGGTGTCTTGATATTATCATCTGCTGAGGGTGGAGCTGAGGGTGGAA615090.15342651553614023No Hit
CATTTGGGTAGTATTCTGGGGTCCGACTATGCGAGTGTGGTGTCATAGAT610570.15229905800923627No Hit
ATTTAGACACCACTCCCCTTATACCCCACCTCCCTTCACCTCCCTATGAT608420.15176276737143904No Hit
AAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTTAATTCCCCT608320.15173782362084381No Hit
AATTTATTCAAGAGAAGGTAAAGAACACTTGCACACATGTGCTAATGTTT605540.15104438735429668No Hit
ATTAAATTTGAAAATCATCACCACCATCCTTAGGAATTTATTCAAGAGAA604110.15068769172078503No Hit
GGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGT599180.1494579648164407No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT598810.14936567293923836No Hit
CAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAG598540.14929832481263128No Hit
CAAGGCCGATTTAGGTGACACTATAGAAGGATGTCAAAGAGATAAATCAA597300.14898902230525055No Hit
TGTAAGTTGTCAGGGTCAATTTAGACACCACTCCCCTTATACCCCACCTC574600.14332679092013556No Hit
TGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTG566050.14119410024424423No Hit
ATTATTGTCTTATTCTATTCTCTTGGATGAATATTAATGTTTCCCGGTTT565910.1411591789934109No Hit
GATTTTTTGAGCTGTGGTCTGTACACTTTATTGTTGTCCATCGTCTGTAT559770.13962763270686437No Hit
GCTGATCTTCGTTAGGTGGGTTAGGGAAATCCGTTGGGGTTCAGAGGTCC551230.13749743640603257No Hit
AGCGGGAACTATGCAAAGGCTGTCTATTCCGTGCGTCTGACTTTATACACAATTACCTGTGTCATGCTGGGGGCA544580.13583867699145044No Hit
TTCCTCCCTTCCTTAATTCCCCTATGGTCCTACTTTACAGTCTGAATAAATTTTACCATATCGGATCATCGAAT544290.1357663401147243No Hit
GGATCATCGAATAGAAGCCGTGACGTATATAAATTAGCCTAGTAACTTCC542770.13538719510567693No Hit
GTTGTCAGGGTCAATTTAGACACCACTCCCCTTATACCCCACCTCCCTTC539750.1346338938377013No Hit
GGGGAAACGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCATTCA533740.13313477442692856No Hit
AATAAAATCGCGCTACCCCGACTGGATACTAGGCCAAATTGGTGAAGAAA532110.13272819129222646No Hit
CATCGAATAGAAGCCGTGACGTATATAAATTAGCCTAGTAACTTCCTTCC529450.1320646875263936No Hit
GATAAAACGAATAGCTCGTAACCAAACATGCACAGCGGTCAAACAGTATG528160.13174291314371525No Hit
CTAAGGTGCTTTCGTGAGTGGCTCTTTCCACCAAGTGCTCGTCCAACTCG527500.13157828438978683No Hit
GTCAGGGTCAATTTAGACACCACTCCCCTTATACCCCACCTCCCTTCACC527120.13148349813752497No Hit
TGAATATTAATGTTTCCCGGTTTTAATGTGTGATTTTTTGAGCTGTGGTC524810.1309072974987754No Hit
GCCCTTCGATCGGGTGTTAGGGGGAAGTAGTTTGATTGTTCGGATAATTATGTTTCACTATGCTCCTCCGTTCCC521530.13008914247925216No Hit
GAACGAGAGAAGGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCA518320.12928844808514559No Hit
AGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCACAC514410.12831314743687247No Hit
TCCAACTCCTCAATCCTATGCTGCTAGCAGTCCCCCCTTCCTCCTGGGGC514050.12822334993472967No Hit
ATTTAATAAAACCCAAACTGTAGTGTGCTTTGTATGCCTTTAGGGTCATC513500.12808615930645598No Hit
GGGAAACGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCATTCAT508830.1269212861536592No Hit
CTCCCTATGATTCGCCCTTTCCCGTCCACAGAGACACGGCTTCAGCATATATTCGCGAACATTCCCAAGTTGGCA495560.12361125044967347No Hit
AGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTC492600.12287291543205495No Hit
CAAAGGCAGAAGCATAGTCCTGATCTATAGTTGGCACCTGTGATTTGTCA492160.12276316292943598No Hit
GAAATAATATCGTTAGCGCCTCGATCCTGGAGAATGACGATACGTTTTTA490960.12246383792229333No Hit
CTAATGTTTCTCTCACCCTTGCATCCTCATTACTCTCTTAGACGCTTGAA488660.12189013165860327No Hit
ATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCA484430.12083501100842546No Hit
GTAGTATCTTCCATTCAGTATTTTATTCGGAAGATGTTAAAAGGAGCAGA482500.1203535966219377No Hit
GTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTTACCA475120.11851274782801045No Hit
ACCTCCCTTCACCTCCCTATGATTCGCCCTTTCCCGTCCACAGAGACACG470270.11730297592414227No Hit
CTTTTATGAAGTTAAAATTAGCTTTATATCAGAATTTCTTATTTTTTAGT466390.11633515840104772No Hit
GTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAG464970.1159809571425956No Hit
GAGCAAGAAAGGATTAAAATGGGGGAAAGGAACGCCCAAGAAGGGGGGCA464900.11596349651717894No Hit
AGGAGATTCAACCCATGCACCAGACCACGAAACTACCGAGTAGTCTGAGA464180.11578390151289333No Hit
AGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCT461520.11512039774706048No Hit
ATAAATTTTACCATATCGGATCATCGAATAGAAGCCGTGACGTATATAAA460090.11476370211354885No Hit
CTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTT459200.11454170273325137No Hit
GGGTCAATTTAGACACCACTCCCCTTATACCCCACCTCCCTTCACCTCCCTATGATTCGCCCTTTCCCGTCCAC456040.11375348021444241No Hit
CGAGTCAATTTAGACACCACTCCCCTTATACCCCACCTCCCTTCACCTCCCTATGATTCGCCCTTTCCCGTCCAC456010.11374599708926386No Hit
GTTGTTATCCATATAAATCATTCTGCTTTGCATGAGTAAGGAGATTCAAC455300.1135688964600378No Hit
CTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCAC454340.11332943645432367No Hit
GGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACC450450.11235912455616962No Hit
CTGTAAGACTGGTACGAGGCCAACATTTCAGGAGGAATTGAGAGAGTCGA446150.11128654328057513No Hit
GGAAGTGGAATTGTTGCCAGGTTGATTCCCCCTCCCAGAGAACCTTTTGTTCTGTGCCTTTTCCCTTCAAGTCTA445430.11110694827628956No Hit
AGGAGAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGA444090.1107727020183136No Hit
ATCTGCTATTCCTTGTTAAATCCGACTATTTAGGCCTAACGGGAATGATG439630.10966021074176678No Hit
CAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTTAATTCCCCTATGGTCCTACTTTACAGTCTGAATAA433460.1081211813300417No Hit
CCTTTCCCGGCAAGGCGGAGGAATTGGGGCAAGAGGTCCCGGGGAAACGA430770.10745019443903026No Hit
ATTAACATTAATTATTGTCTTATTCTATTCTCTTGGATGAATATTAATGT430390.10735540818676843No Hit
AGTTAAGTGTATTAAATTTGAAAATCATCACCACCATCCTTAGGAATTTA425410.10611320940712647No Hit
ATTAATTATTGTCTTATTCTATTCTCTTGGATGAATATTAATGTTTCCCG423980.10575651377361481No Hit
GTTAAAAGGAGCAGAAATAGCGATATAAAAGCATTCTTCACTGGCATGTG416930.10399797935665177No Hit
GGAGAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAA414180.10331202621528322No Hit
TATAAATTAGCCTAGTAACTTCCTTCCTGCCCCGACCTAGACGCACTGTAAGTTGTCAGGGTCAATTTAGACACC413020.10302267870837868No Hit
TATGTTAATTTGAAAATCATCACCACCATCCTTAGGAATTTATTCAAGAG407980.10176551367837958No Hit
CTCCCCTTATACCCCACCTCCCTTCACCTCCCTATGATTCGCCCTTTCCCGTCCACAGAGACACGGCTTCAGCAT406980.10151607617242737No Hit
AGAACGAGAGAAGGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCACTGCCGCGTCTGACGGGACAAGGG406960.10151108742230833No Hit
CAACCTTTCCCGGCAAGGCGGAGGAATTGGGGCAAGAGGTCCCGGGGAAA404960.10101221241040391No Hit
CCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTG403770.10071538177832079No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)