FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005284421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5207876
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA282651254.27379607348563No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA2298304.413123507548951No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGAATTCTCGGGTGCC807021.5496144685472542No Hit
TCTTAACCCGGACCAGAAACTATGGAATTCTCGGGTGCCAAGGAACTCCA383270.7359430216848482RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA374240.7186038991711784RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGC324600.6232867295611493RNA PCR Primer, Index 12 (100% over 50bp)
AGGTTCCGGATAAGTAAGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCA319310.6131290376345366RNA PCR Primer, Index 1 (100% over 28bp)
ATTCCCGTGTGAAAGTAGGTAATCGTCAGGCTTGGAATTCTCGGGTGCCA295760.5679090669593515No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA281820.5411419165894118RNA PCR Primer, Index 1 (100% over 23bp)
GTCCCACTCCGTAGATCTGTTCTGGAATTCTCGGGTGCCAAGGAACTCCA237620.4562704641969202RNA PCR Primer, Index 1 (100% over 28bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG220540.4234739844036225No Hit
CCCGTGTGAAAGTAGGTAATCGTCAGGCTTGGAATTCTCGGGTGCCAAGG187480.3599932102838086Illumina Small RNA Adapter 2 (100% over 21bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG186930.35893711755041785RNA PCR Primer, Index 1 (100% over 29bp)
GATTCCCGTGTGAAAGTAGGTAATCGTCAGGCTTGGAATTCTCGGGTGCC185880.35692094051394463No Hit
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC163270.3135059283285547RNA PCR Primer, Index 1 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACATGGAATTCTCGGGTGCCA148640.28541386162036114No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA140090.2689964200376507RNA PCR Primer, Index 1 (100% over 28bp)
GCTCTACACTCTACTCGTCAGCTGTTGGAATTCTCGGGTGCCAAGGAACT139520.26790192393213663RNA PCR Primer, Index 1 (100% over 25bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC134500.25826267752918847RNA PCR Primer, Index 1 (100% over 26bp)
CCGTGTGAAAGTAGGTAATCGTCAGGCTTGGAATTCTCGGGTGCCAAGGA128540.24681847263644524RNA PCR Primer, Index 1 (100% over 22bp)
GGATTCCCGTGTGAAAGTAGGTAATCGTCAGGCTTGGAATTCTCGGGTGC107550.20651413359304255No Hit
TCCCGTGTGAAAGTAGGTAATCGTCAGGCTTGGAATTCTCGGGTGCCAAG103900.1995055181805404No Hit
CGGATTCCCGTGTGAAAGTAGGTAATCGTCAGGCTTGGAATTCTCGGGTG103610.19894866928475255No Hit
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC102890.19756614788831375RNA PCR Primer, Index 1 (100% over 27bp)
CGATGCTATAGACTCTCACGTGATGTGGAATTCTCGGGTGCCAAGGAACT91720.17611786455745107RNA PCR Primer, Index 1 (100% over 25bp)
TGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA90950.17463933473070403No Hit
TATGCATATGATCACGAGACTCAGTTGGAATTCTCGGGTGCCAAGGAACT84750.16273428937248122RNA PCR Primer, Index 1 (100% over 25bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTATCGGGTGCCA79140.15196214349189574No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC76970.14779537761651773RNA PCR Primer, Index 1 (100% over 27bp)
TTCTTACTCGTCTATCGGCTAGGATGCGCGCCTTGGAATTCTCGGGTGCC76630.14714252029042166No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTTGGAATTCTCGGGTGCCAAGGA76520.1469313017437435RNA PCR Primer, Index 1 (100% over 22bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTTGGAATTCTCGGGT74480.14301415778716697No Hit
GGGTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA71940.13813692952750795No Hit
TCTTACTCGTCTATCGGCTAGGATGCGCGCCTTGGAATTCTCGGGTGCCA63820.12254516044544839No Hit
TTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCC63220.12139305928174941No Hit
GGATGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA62710.12041377329260529No Hit
AGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA58840.11298272078674684No Hit
CGTGTGAAAGTAGGTAATCGTCAGGCTTGGAATTCTCGGGTGCCAAGGAA58530.1123874685188357RNA PCR Primer, Index 1 (100% over 23bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC58040.11144658590181487RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC57210.10985284595869794RNA PCR Primer, Index 1 (100% over 26bp)
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGC54980.10557086996695006No Hit
CGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA54220.10411154182626467No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA53870.10343948281410695RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA52380.10057843159092114RNA PCR Primer, Index 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACC150.002223035670.018341
CGCTAGG150.002224390670.007584
CTTCATC150.002225322670.0001942
CCCCTCG401.3460522E-1069.9988527
GGGATAT950.069.99750519
ACGATTC150.002225831269.996168
GTCTAGC2200.069.9954810
GGCTGGT3351750.069.977611
CTGGTCC3385900.069.8545763
GCTGGTC3379750.069.8505252
TGGTCCG3426300.069.8461764
GGTCCGA3438350.069.793795
GTCCGAT3427150.069.775076
TCCGATG3429200.069.7457667
AGTGGGT3440850.069.7076416
GATGGTA3441900.069.7046810
GGTAGTG3442100.069.7036813
TAGTGGG3441100.069.70257615
GGGTTAT3457350.069.69887519
TGGGTTA3457200.069.6978618
Produced by FastQC (version 0.11.5)