FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005284444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6519328
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA377553957.913008825449495No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA2573793.9479375788424824No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGAATTCTCGGGTGCC1504812.308228700872237No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA1110811.7038719328127072RNA PCR Primer, Index 1 (100% over 23bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA665261.0204425977646776RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACATGGAATTCTCGGGTGCCA392750.6024393925263463No Hit
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG306640.4703552267963815RNA PCR Primer, Index 1 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTTGGAATTCTCGGGT300620.4611211462285683No Hit
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC281200.43133279994502505RNA PCR Primer, Index 1 (100% over 27bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG279590.4288632202582843No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTTGGAATTCTCGGGTGCCAAGGA270470.41487404836817543RNA PCR Primer, Index 1 (100% over 22bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGC217090.33299444359909486No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA216800.3325496124753962RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTATGGAATTCTCGGG197340.30269991017479103No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA193530.29685574954964683RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC187540.2876676859946301RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC164800.25278679029495066RNA PCR Primer, Index 1 (100% over 26bp)
GCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCATGGAATTCTCGG143960.22082030540571054No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATGGAATTCTCGGGTGC142650.21881089584693392No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAACTTGGAATTCTC125270.19215170643354654No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAACTGGAATTCTCG120570.18494237442877548No Hit
TGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA118440.1816751665202303No Hit
GCTCTACACTCTACTCGTCAGCTGTTGGAATTCTCGGGTGCCAAGGAACT114380.17544753078844935RNA PCR Primer, Index 1 (100% over 25bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTATCGGGTGCCA107620.16507836390499145No Hit
GGGTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA93080.14277545170299763No Hit
CCCCCCACTGCTAAATTTGACTGGCTATGGAATTCTCGGGTGCCAAGGAA92790.14233062057929896RNA PCR Primer, Index 1 (100% over 23bp)
CGATGCTATAGACTCTCACGTGATGTGGAATTCTCGGGTGCCAAGGAACT91920.14099612720820304RNA PCR Primer, Index 1 (100% over 25bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA87960.1349218815190768RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC86970.13340332009679523RNA PCR Primer, Index 1 (100% over 27bp)
GGATGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA80270.12312618723893014No Hit
CCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAAC79630.12214449096594004RNA PCR Primer, Index 1 (100% over 24bp)
AGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA78050.11972092829199572No Hit
TACAACCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCA70890.108738201237919No Hit
CGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA68540.10513353523553348No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACCC150.002226088269.9943214
GGCTGGT4556050.069.9619451
GCTGGTC4595300.069.837092
TGGTCCG4667300.069.830964
CTGGTCC4604850.069.816693
GGTCCGA4678700.069.796235
GTCCGAT4654200.069.780086
TCCGATG4656650.069.7602547
CGATGGT4667750.069.7397169
AGTGGGT4672500.069.7349516
TAGTGGG4673150.069.73048415
GGTAGTG4675750.069.720913
TGGGTTA4704050.069.7206518
GGGTTAT4704150.069.71895619
GAACTTG4027550.069.7056228
GTGGGTT4705500.069.7033117
TCAGAAC4658000.069.7025725
TGGTAGT4675050.069.6991612
GATGGTA4675450.069.69768510
ATGGTAG4676150.069.6889611
Produced by FastQC (version 0.11.5)