FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005284448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30442659
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA4192851.377294276429664No Hit
GTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGT4087941.342832766349352No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACAC2378300.7812392472024208No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2049380.6731934946944024No Hit
CACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCA2045910.672053646825003No Hit
AATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATG1960930.644138871049339No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCA1771230.581824997612725No Hit
GGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACC1653530.5431621462501025No Hit
CCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGAGGGTTC1481750.4867347494185708No Hit
GTTTACCAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCT1457130.4786474138149365No Hit
GCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGG1433000.4707210365559723No Hit
GGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGT1144570.37597569910039724No Hit
CAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAG1100850.3616142729188012No Hit
GTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAG978920.3215619240093318No Hit
TGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAAT961100.3157082960460188No Hit
CCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTG936160.3075158447887223No Hit
CGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGG887090.2913970162724616No Hit
GGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCG880240.28914688431125546No Hit
GTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATA878320.2885161903892824No Hit
CTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAAAAATCCACTGCCTTCCAA826220.27140204802740786No Hit
CCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACT751100.24672614833020995No Hit
GAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATT743430.24420665750649442No Hit
CAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATA716970.2355149068943025No Hit
ATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGC677450.22253312366702266No Hit
GGCAAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACC646650.21241574200203733No Hit
CTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGC630240.2070252798876734No Hit
TATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAA610540.20055409745909514No Hit
CAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCA586400.19262443533595405No Hit
CACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGC565820.18586418485980477No Hit
GCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACAC561790.18454038459649666No Hit
CCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTC553000.18165298898496351No Hit
ACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTT516900.16979462930619826No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT515450.16931832400054148No Hit
ATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTG508870.1671568833721128No Hit
ACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGAGGGTTC495150.1626500497213466No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT483250.1587410613507841No Hit
AAAACGAATAGCTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCC473660.1555908766050955No Hit
TGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAA473140.15542006366789446No Hit
AGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGA465920.15304839173214138No Hit
CATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATG464180.15247682536535329No Hit
ACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCA458760.15069642898145No Hit
AGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCAT454100.14916568227499444No Hit
GGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA453110.14884048072147704No Hit
GTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTT411390.13513602737526967No Hit
GTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAAGACG390630.12831664934393544No Hit
CACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCA387840.12740017223856825No Hit
ATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCC381330.12526172565937818No Hit
CCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCAT378170.12422370857946409No Hit
AAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGT371520.12203927390179682No Hit
ACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCAC364980.1198909727300759No Hit
AACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTTACCA364870.11985483922412954No Hit
CAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAA359780.1181828433580654No Hit
GACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT350710.11520347154957784No Hit
CCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAAATAGGGACCTGTATGAATGGCTCCACGAGGGTTC334590.10990827049634527No Hit
CATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCT332330.10916589119235609No Hit
CTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCAC330720.10863702805986822No Hit
CAATAACGTCTCTTTCAGAAGGCATTGGTATCTTTTCCCCACTTCCAAGC314330.1032531356738582No Hit
GTCATTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAA313280.10290822493527914No Hit
AGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATT312030.10249761691316123No Hit
GGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGG309650.10171581923904872No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA305220.10026062440866286No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA8500.056.68226665
GTTAGCG10350.045.6017211
ATGCGTA12100.045.31711215
GTTCGCG9400.045.12999755
GCGTAAG12950.045.02328517
CGTAAGC12900.044.92920318
TTCGCGA13400.044.40717758
CGCGAAC13650.043.10716260
ACGCGAC10500.043.0612756
ATTCGCG13600.042.97305357
TAGCGTC15100.042.76037640
AACGCGA10500.042.72024555
CGTAATC11450.042.276667
TACGTAA13800.042.2468453
TAGTCCG4700.042.17065455
CATACTC31950.042.05537859
TTATACG7900.041.8333257
CGAACAT13900.041.6113662
TAGCGAG8950.041.1081928
TCGCGAA14500.040.33168859
Produced by FastQC (version 0.11.5)