FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005284449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5850677
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA281509648.11573088037504No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA2318473.9627379874158155No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGAATTCTCGGGTGCC830041.4187076128113036No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA501300.8568239196933962RNA PCR Primer, Index 1 (100% over 23bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA451220.7712269879195177RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG257080.43940214098300073RNA PCR Primer, Index 1 (100% over 29bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG229480.3922281130884511No Hit
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC192280.3286457276653625RNA PCR Primer, Index 1 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACATGGAATTCTCGGGTGCCA153090.26166202646292047No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGC137190.23448568430627773RNA PCR Primer, Index 10 (100% over 50bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC133840.2287598512103813RNA PCR Primer, Index 1 (100% over 26bp)
TCTTAACCCGGACCAGAAACTATGGAATTCTCGGGTGCCAAGGAACTCCA120020.20513865318492203RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC118920.20325852888477694RNA PCR Primer, Index 1 (100% over 27bp)
GCTCTACACTCTACTCGTCAGCTGTTGGAATTCTCGGGTGCCAAGGAACT112940.1930374895076245RNA PCR Primer, Index 1 (100% over 25bp)
AGGTTCCGGATAAGTAAGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCA102590.17534722904716837RNA PCR Primer, Index 1 (100% over 28bp)
CCCCCCACTGCTAAATTTGACTGGCTTTTGGAATTCTCGGGTGCCAAGGA99900.17074947053135903RNA PCR Primer, Index 1 (100% over 22bp)
TGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA84320.1441200736256676No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTATCGGGTGCCA81840.13988124793079504No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC75040.1282586613480799RNA PCR Primer, Index 1 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTTGGAATTCTCGGGT71630.12243027601763011No Hit
GGGTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA70010.11966136568468913No Hit
GTCCCACTCCGTAGATCTGTTCTGGAATTCTCGGGTGCCAAGGAACTCCA69080.11807180604911191RNA PCR Primer, Index 1 (100% over 28bp)
CGATGCTATAGACTCTCACGTGATGTGGAATTCTCGGGTGCCAAGGAACT58700.10033026947137913RNA PCR Primer, Index 1 (100% over 25bp)
GGATGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA58610.10017644111954907No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTGGT3347100.069.972171
CTGGTCC3378000.069.865473
TGGTCCG3422900.069.857154
GCTGGTC3373850.069.8497242
GTCCGAT3413750.069.816746
GGTCCGA3432750.069.805595
TCCGATG3416150.069.797017
AGTGGGT3425900.069.7760416
GGGTTAT3446500.069.773219
CGATGGT3422200.069.769669
TGGGTTA3447950.069.73717518
GTTATCA3419450.069.7346821
TAGTGGG3428800.069.7292715
GATGGTA3428300.069.7271910
ATCAGAA3422100.069.70965624
GTGGGTT3450000.069.7045117
TGGTAGT3429800.069.6946612
TTATCAG3421100.069.693222
GGTAGTG3431250.069.6825413
TATCAGA3422150.069.6769323
Produced by FastQC (version 0.11.5)