FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005284450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4939354
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA1931473.9103696556270315No Hit
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA529621.0722454798744938RNA PCR Primer, Index 1 (100% over 34bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA377940.7651607882326312RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA303040.6135215252844806No Hit
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG152340.3084208987653041RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC146500.2965974902790932RNA PCR Primer, Index 1 (100% over 27bp)
TTCGACCTGAGCTCAGGCAAGGCTACCCTGGAATTCTCGGGTGCCAAGGA96440.19524820452229177RNA PCR Primer, Index 1 (100% over 22bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC91920.18609721028296414RNA PCR Primer, Index 1 (100% over 50bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT87840.17783702079259758RNA PCR Primer, Index 1 (100% over 35bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG86950.176035165732199Illumina Small RNA Adapter 2 (100% over 21bp)
GTCCCACTCCGTAGATCTGTTCTGGAATTCTCGGGTGCCAAGGAACTCCA81680.16536575430714218RNA PCR Primer, Index 1 (100% over 28bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATC78020.15795587844078396RNA PCR Primer, Index 1 (100% over 36bp)
AGGTTCCGGATAAGTAAGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCA73170.14813678063973548RNA PCR Primer, Index 1 (100% over 28bp)
TCTTAACCCGGACCAGAAACTATGGAATTCTCGGGTGCCAAGGAACTCCA64840.13127222709690378RNA PCR Primer, Index 1 (100% over 28bp)
GTGTTCGGAAGATTTTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC64640.130867315847376RNA PCR Primer, Index 1 (100% over 33bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA63280.12811391935058714RNA PCR Primer, Index 1 (100% over 28bp)
CAACTCTCAACAACGGATATCTTGGCTCTTGGAATTCTCGGGTGCCAAGG53060.10742295449971799Illumina Small RNA Adapter 2 (100% over 21bp)
TTCGACCTGAGCTCAGGCAAGGCTACCCGTGGAATTCTCGGGTGCCAAGG51670.10460882131549996Illumina Small RNA Adapter 2 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)