FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005284453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50377369
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGTCTTGCTGTGTCATGTCCGCCTCTTCACGGGCAGGTCAATTTCACT2090560.41497998833563543No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT1569340.3115168638520999No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGTCCGCCTCTTC1273190.2527305465277474No Hit
GTCTTGCTGTGTCATGTCCGCCTCTTCACGGGCAGGTCAATTTCACTGGT1189800.23617747881990422No Hit
GGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCT1151930.22866021447050955No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC1135660.22543058967609045No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGC1043990.20723392680550667No Hit
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT856570.17003071359284364No Hit
GTCATGTCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG851410.16900644414359947No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGC792130.15723925558716653No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGC751880.14924955687939956No Hit
CCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA708550.14064847253138607No Hit
GGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTT679870.13495544001116852No Hit
GGGTCTTCTCGTCTTGCTGTGTCATGTCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGAC646980.1284267147813932No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG640550.12715034800646297No Hit
GTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGG622500.12356738995242089No Hit
GGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTC619420.12295600431217438No Hit
CACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACA587450.11660990076714806No Hit
GCTGTGTCATGTCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG578720.11487697978034542No Hit
GGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTG551440.10946184982387626No Hit
CTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGG538030.10679994026682893No Hit
CTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATG536460.10648829239176823No Hit
CTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGTCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA535890.10637514634795636No Hit
GTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCC526240.10445960367640478No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTCTAATACTGGTG513940.10201803115204369No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)