FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005284455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1240360
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT631995.095214292624721RNA PCR Primer, Index 3 (100% over 34bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA620245.000483730529846RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA403423.252442839175723No Hit
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG363022.9267309490792996RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC180281.4534489986778032RNA PCR Primer, Index 1 (100% over 27bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA89720.7233383856299784RNA PCR Primer, Index 7 (97% over 35bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA52390.422377374310684No Hit
ATATACGGAGAGTTTGATTCTGGCTCTGGAATTCTCGGGTGCCAAGGAAC46250.37287561675642555RNA PCR Primer, Index 1 (100% over 24bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAG46160.3721500209616563RNA PCR Primer, Index 7 (97% over 36bp)
GTCCCACTCCGTAGATCTGTTCTGGAATTCTCGGGTGCCAAGGAACTCCA45010.3628785191396046RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG43430.35014028185365537Illumina Small RNA Adapter 2 (100% over 21bp)
AGGTTCCGGATAAGTAAGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCA43050.3470766551646296RNA PCR Primer, Index 1 (100% over 28bp)
TCTTAACCCGGACCAGAAACTATGGAATTCTCGGGTGCCAAGGAACTCCA42820.3452223548002193RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC37920.3057176948627818RNA PCR Primer, Index 1 (100% over 27bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA37780.30458899029314074RNA PCR Primer, Index 1 (100% over 22bp)
ATATACGGAGAGTTTGATTCTGGCTCATGGAATTCTCGGGTGCCAAGGAA35410.28548163436421686RNA PCR Primer, Index 1 (100% over 23bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA34380.2771775936018575RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA31300.2523460930697539RNA PCR Primer, Index 1 (100% over 28bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC30180.24331645651262537RNA PCR Primer, Index 1 (100% over 33bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA25280.20381179657518786RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT25230.203408687800316RNA PCR Primer, Index 1 (100% over 30bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGC24960.20123190041600827RNA PCR Primer, Index 10 (100% over 50bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA24610.19841013899190557RNA PCR Primer, Index 1 (100% over 32bp)
AAGATTAAGAGAACCAACACCTTGGAATTCTCGGGTGCCAAGGAACTCCA24150.19470153826308492RNA PCR Primer, Index 1 (100% over 28bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA23390.18857428488503336RNA PCR Primer, Index 1 (100% over 23bp)
AAACTTGACCGTACTGTAAACCGCATCATGGAATTCTCGGGTGCCAAGGA23280.1876874455803154RNA PCR Primer, Index 1 (100% over 22bp)
AAACCGTTACCATTACTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG21610.1742236124995969RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCTGGAATTCTCGGGTGCCAAGGAA21180.1707568770356993RNA PCR Primer, Index 1 (100% over 23bp)
GGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCAAGGA19700.1588248572994937RNA PCR Primer, Index 1 (100% over 22bp)
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA19030.15342319971621143RNA PCR Primer, Index 1 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTCTCGGGTGCCAAGG18250.14713470282821117Illumina Small RNA Adapter 2 (100% over 21bp)
ATACGATGAAGAGTTTGATCCTGGCTCATGGAATTCTCGGGTGCCAAGGA16640.13415460027733883RNA PCR Primer, Index 1 (100% over 22bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC16290.13133283885323616RNA PCR Primer, Index 1 (100% over 24bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG16250.1310103518333387No Hit
AAAGATTAAGAGAACCAACACCTTGGAATTCTCGGGTGCCAAGGAACTCC15730.12681802057467187RNA PCR Primer, Index 1 (100% over 27bp)
CGGACAGGATTGACAGATTGATAGCTTGGAATTCTCGGGTGCCAAGGAAC15440.12447998968041538RNA PCR Primer, Index 1 (100% over 24bp)
ATACGATGAAGAGTTTGATCCTGGCTCTGGAATTCTCGGGTGCCAAGGAA14080.11351543100390209RNA PCR Primer, Index 1 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCTGGAATTCTCGGGTGCCAAGGA13870.11182237414944049RNA PCR Primer, Index 1 (100% over 22bp)
TTCGACCTGAGCTCAGGCAAGGCTACCCGTGGAATTCTCGGGTGCCAAGG13660.11012931729497888Illumina Small RNA Adapter 2 (100% over 21bp)
TCCTCGATCGTATAATGGTTATTATTTGGAATTCTCGGGTGCCAAGGAAC13420.10819439517559418RNA PCR Primer, Index 1 (100% over 24bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG13420.10819439517559418RNA PCR Primer, Index 1 (100% over 29bp)
CAAACGAGAACTTTGAAGGCCGAAGTGGAATTCTCGGGTGCCAAGGAACT13240.10674320358605566RNA PCR Primer, Index 1 (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12860.10367957689702989No Hit
GTGTGAAAGTAGGTCATCGTCAGGCTTGGAATTCTCGGGTGCCAAGGAAC12790.10311522461220937RNA PCR Primer, Index 1 (100% over 24bp)
GTTCGGGAAGTCCAGGTGCTGTTAACTTTGGAATTCTCGGGTGCCAAGGA12520.10093843722790156RNA PCR Primer, Index 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG150.002220821670.032751
TTCCACG150.002225088269.9988726
TCTACAC1300.069.996053
TGTACCG150.00222544469.996054
GTGCAAC150.002226511569.9875911
AACAGCG150.002226511569.987599
TACGGCC9950.069.644314
CTACGGC9700.069.635253
CCTGTAC140300.069.402962
TGCCCCG140350.069.1647914
CTGCCCC140400.069.1152313
GCCCCGA137050.069.0939115
TCCTGTA139500.068.97851
GAGCTGC140750.068.9682210
GCTGCCC140550.068.9667812
CCCCGAG95050.068.9567316
GTCTACG9750.068.955331
GGCCATA10100.068.9481667
ACGGCCA10000.068.9461065
CTGTACT140600.068.900813
Produced by FastQC (version 0.11.5)