FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005284465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30004217
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA796504126.546405126985984No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA15989355.329034248752434No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA6843462.280832724280057RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA6813032.2706908165608852RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTGCCAAGGAACTCC5857341.9521722563198365RNA PCR Primer, Index 1 (100% over 27bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT4088151.3625251410493397RNA PCR Primer, Index 1 (100% over 30bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC3534781.178094399197286RNA PCR Primer, Index 1 (100% over 27bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA2891230.9636078821853609RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC2779080.9262298029640301RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG2700000.8998735077805896RNA PCR Primer, Index 1 (100% over 29bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA2273320.7576668306325075RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA1872150.62396229170053RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGAATTCTCGGGTGCC1765010.5882539777658586No Hit
TGAGGGGCAGAGAGCGAGACTTGGAATTCTCGGGTGCCAAGGAACTCCAG1744200.5813182860262609RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1677790.5591847305997021RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC1604410.5347281683771318RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA1502180.5006562910806838RNA PCR Primer, Index 1 (100% over 28bp)
AAATGGATTTTTGGAGCAGGGAGATGGAATTCTCGGGTGCCAAGGAACTC1445260.4816856243907315RNA PCR Primer, Index 1 (100% over 26bp)
GGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCAAGGA1411880.47056052154268846RNA PCR Primer, Index 1 (100% over 22bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACATGGAATTCTCGGGTGCCA1344370.44806035098333014No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC1146410.3820829585387947RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGGGCAGAGAGCGAGACTTTGGAATTCTCGGGTGCCAAGGAACTCCA1138440.37942666525842017RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC1105580.368474871382246RNA PCR Primer, Index 1 (100% over 26bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC1060290.35338032650543755RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1025200.34168530376913353RNA PCR Primer, Index 1 (100% over 30bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGC1019020.3396255932957691No Hit
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG962920.3209282215229946RNA PCR Primer, Index 1 (100% over 29bp)
CCCATAAAGTAGAAAGCACTACTGGAATTCTCGGGTGCCAAGGAACTCCA884560.2948118926082957RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA845930.281937035717346RNA PCR Primer, Index 1 (100% over 28bp)
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA844350.28141044307205215RNA PCR Primer, Index 1 (100% over 23bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAG786520.2621364856813294No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA774850.2582470324088111No Hit
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG758060.25265115233635327RNA PCR Primer, Index 6 (100% over 34bp)
CATTGCACTTGTCTCGGTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT756840.2522445428254302RNA PCR Primer, Index 1 (100% over 30bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG749370.2497548927872372Illumina Small RNA Adapter 2 (100% over 21bp)
AAGCTGCCAGTTGAAGAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAG707380.23576019330882725RNA PCR Primer, Index 1 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC703970.23462368639714878No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA688180.22936109280905412RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGGGCAGAGAGCGAGACTTTTTGGAATTCTCGGGTGCCAAGGAACTC665980.22196213285619154RNA PCR Primer, Index 1 (100% over 26bp)
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGTGCCAAGGAACTCC609890.20326809394826065RNA PCR Primer, Index 1 (100% over 27bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG582310.19407605270952413No Hit
GTCAGGATGGCCGAGCGGTCTAAGGCTGGAATTCTCGGGTGCCAAGGAAC562530.1874836460488204RNA PCR Primer, Index 1 (100% over 24bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG527590.17583861628517083RNA PCR Primer, Index 1 (100% over 29bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGGAATTCTCGGGTGCCAA526700.1755419913140876No Hit
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG518640.17285570225012037RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA511700.1705426940486399RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC507140.16902290767994377No Hit
CAAATAAAGTATAGGCGATAGAAATTGAAACCTGGAATTCTCGGGTGCCA483470.1611340165950673No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT481680.16053743378805718RNA PCR Primer, Index 1 (100% over 30bp)
GGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAAGGAA457400.15244523794771916RNA PCR Primer, Index 1 (100% over 23bp)
AAATGGATTTTTGGAGCAGGGAGATTGGAATTCTCGGGTGCCAAGGAACT446290.1487424251064442RNA PCR Primer, Index 1 (100% over 25bp)
CTAGACTGAAGCTCCTTGAGGATGGAATTCTCGGGTGCCAAGGAACTCCA438400.1461127947448187RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGAAGGTAGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCC435680.14520625550735083RNA PCR Primer, Index 1 (100% over 27bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA426450.14213002125667867RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTATCGGGTGCCA412200.13738068885450336No Hit
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCC382640.1275287403767277No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA368140.12269608635346158RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA361720.12055638712384995RNA PCR Primer, Index 1 (100% over 28bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC361230.120393076746512RNA PCR Primer, Index 1 (100% over 31bp)
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTC354990.11831336908408574RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG340900.11361736251940852RNA PCR Primer, Index 1 (100% over 29bp)
AGTTGGTCCGAGTGTTGTGGGTTATTGTTGGAATTCTCGGGTGCCAAGGA339860.11327074457567082RNA PCR Primer, Index 1 (100% over 22bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG330230.11006119573125338RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCAA321930.10729491791103896No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTTGGAATTCTCGGGT318310.10608842083764426No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTGGT11552800.069.949091
GCTGGTC11599700.069.8378752
TGGTCCG11814000.069.835774
CTGGTCC11579200.069.8357543
CTACGGC177700.069.700133
TGAGGGG1172700.069.696041
AGGGGCA1200950.069.685943
TCAGTGC379850.069.656021
AAGCTGC891150.069.6540451
GGGCAGA1200850.069.6392755
GGGGCAG1203700.069.6198044
AGTGCAC369550.069.570113
GAGCGAG1191250.069.5673412
TACGGCC178200.069.56354
AGAGAGC1193200.069.562189
CCTGTAC1797550.069.542092
TGCCAGT903250.069.530875
GCAGAGA1198650.069.516247
AGAGCGA1191050.069.5114311
TCCTGTA1776750.069.487411
Produced by FastQC (version 0.11.5)