FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005284468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48581483
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGTCCGCCTCTTC1035250.21309559446754642No Hit
GGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTC996720.20516458915015007No Hit
GGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTT962800.19818250505032955No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT844130.17375550268813325No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGC820250.1688400496131417No Hit
GTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGG763240.15710512583570163No Hit
GGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCT759990.15643614666929787No Hit
GTCATGTCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG753790.15515994025954294No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT693580.14276632930287453TruSeq Adapter, Index 7 (97% over 35bp)
GTCTTGCTGTGTCATGTCCGCCTCTTCACGGGCAGGTCAATTTCACTGGT622980.12823404341114905No Hit
GTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTC598710.12323831283618904No Hit
CTCGTCTTGCTGTGTCATGTCCGCCTCTTCACGGGCAGGTCAATTTCACT594040.12227704123400267No Hit
CGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATT538250.11079324194364343No Hit
GGGTCTTCTCGTCTTGCTGTGTCATGTCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGAC522430.10753685720133327No Hit
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT522330.1075162732269824No Hit
GGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTG507650.10449454579227234No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)