FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005284472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10205874
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC135857113.31165758072263No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA131863512.920353514064548No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA4226904.141634513614415No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA1439701.4106582150632077No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA1334901.3079722520579815No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA1091011.0690020276558383No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC943660.9246243878770207No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA824200.807574147985758RNA PCR Primer, Index 1 (100% over 23bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC799990.7838525147380813No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA643680.6306956170534733No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA608590.5963134563487654No Hit
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGC541860.5309295411642354No Hit
CTGGACGCGAGCCGGGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT530410.5197105118091797RNA PCR Primer, Index 1 (100% over 30bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG506460.49624363381323344Illumina Small RNA Adapter 2 (100% over 21bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGGAATTCTCGGGTGC478600.46894562876241663No Hit
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG473240.46369375126520274RNA PCR Primer, Index 6 (100% over 34bp)
GTTTCCGTAGTGTAGTGGTTATCACTTTCGCCTTGGAATTCTCGGGTGCC457750.4485162172294112No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTGGAATTCTCGGGTGC421590.41308564068104314No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC409720.4014550836116534No Hit
CGCGACCTCAGATCAGACGTGGCGACCTGGAATTCTCGGGTGCCAAGGAA353130.34600662324461384RNA PCR Primer, Index 1 (100% over 23bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA337870.3310544496238147No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGAATTCTCGGGTGCC328120.3215011276839201No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG326800.320207754867442No Hit
CTGGACGCGAGCCGGGCCCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG321650.31516164122739515RNA PCR Primer, Index 1 (100% over 29bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC289750.28390513149584246RNA PCR Primer, Index 1 (100% over 31bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA285460.2797016698422889RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG264210.258880327152775No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCA253170.24806302723314044No Hit
GGCTGGTCCGATGGTAGTGGGTTATCTGGAATTCTCGGGTGCCAAGGAAC246580.24160596142966295RNA PCR Primer, Index 1 (100% over 24bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC232440.2277511950470876No Hit
GCGGCGACTCTGGACGCGAGCCGGGCCCTGGAATTCTCGGGTGCCAAGGA211090.20683186956844657RNA PCR Primer, Index 1 (100% over 22bp)
GCGGCGACTCTGGACGCGAGCCGGGCCTGGAATTCTCGGGTGCCAAGGAA204650.20052177794865977RNA PCR Primer, Index 1 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA201170.19711197688703583No Hit
CTGGACGCGAGCCGGGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC195270.1913309923285355RNA PCR Primer, Index 1 (100% over 31bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA193360.18945952105620742RNA PCR Primer, Index 1 (100% over 22bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG164400.16108370532499228RNA PCR Primer, Index 1 (100% over 29bp)
CCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAACT163230.1599373066922049RNA PCR Primer, Index 1 (100% over 25bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC161020.15777188705249545No Hit
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC160520.15728197310685982RNA PCR Primer, Index 1 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC158110.15492058788889612No Hit
CCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAAC157860.15467563091607833RNA PCR Primer, Index 1 (100% over 24bp)
GGGCGCGCGCCGCGGCTGGACTGGAATTCTCGGGTGCCAAGGAACTCCAG149200.14619032137766938RNA PCR Primer, Index 1 (100% over 29bp)
CGGCGACTCTGGACGCGAGCCGGGCCCTGGAATTCTCGGGTGCCAAGGAA149080.14607274203071682RNA PCR Primer, Index 1 (100% over 23bp)
CGGCCCCGCCGGGGTCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT144900.14197706144520303RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA143070.14018397640417665No Hit
GCGGCGACTCTGGACGCGAGCCGGGCCCTTGGAATTCTCGGGTGCCAAGG142960.1400761953361368Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC139270.13646063041734594No Hit
CTGGACGCGAGCCGGGCCCTTCCCGTGGATTGGAATTCTCGGGTGCCAAG129260.1266525532257208No Hit
GTTTCCGTAGTGTAGTGGTTATCACATTCGCCTTGGAATTCTCGGGTGCC127980.1253983735248936No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGC125360.12283122444976295No Hit
CTGGACGCGAGCCGGGCCCTTCTGGAATTCTCGGGTGCCAAGGAACTCCA124870.12235110878304004RNA PCR Primer, Index 1 (100% over 28bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA124650.12213554664696037RNA PCR Primer, Index 1 (100% over 23bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA119680.1172658020273423No Hit
GGCGGCGACTCTGGACGCGAGCCGGGCCCTGGAATTCTCGGGTGCCAAGG112380.11011305842106223Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCAA111770.10951536340738675No Hit
ACGGCCCTGGCGGAGCGCTGAGAAGACGTGGAATTCTCGGGTGCCAAGGA109040.10684043326421627RNA PCR Primer, Index 1 (100% over 22bp)
GGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCAAGGA108690.10649749350227133RNA PCR Primer, Index 1 (100% over 22bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG107550.10538048970622212No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC107120.10495916371297549No Hit
TAAGCAGGGTCGGGCCTGGTTAGTACTTGGATGGTGGAATTCTCGGGTGC106720.104567232556467No Hit
GCGGCGACTCTGGACGCGAGCCGGGCCCTTTGGAATTCTCGGGTGCCAAG106150.10400873065844238No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA103330.10124561600505746No Hit
GTTCTATTTTGTTGGTTTTCGGAACTTGGAATTCTCGGGTGCCAAGGAAC103320.10123581772614476RNA PCR Primer, Index 1 (100% over 24bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG102900.10082429001181084Illumina Small RNA Adapter 2 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)