FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005284475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26547244
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAAAAATCCACTGCCTTCCAA2889201.088323895316591No Hit
CTGACAAACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAAAA1261930.4753525450702152No Hit
GGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAAAAATCCACTGCCTTCCAA897310.338004954487931No Hit
GTCTGACAAACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAA866760.32649716859497735No Hit
TGACAAACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGA820880.30921477197407005No Hit
CCTCGCTTCGCTTTTCCTCCGCAACCATGTCTGACAAACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGA584080.22001530554358112No Hit
CCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGAC579810.21840685232711915No Hit
CGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAAAA554020.2086920962492378No Hit
CAGCATTCGGGCCGAGATGTCTCGCTCCGTGGCCTTAGCTGTGCTCGCGC546270.20577277249570614No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCA488410.18397766638224292No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA459470.17307634645615189No Hit
CCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTT440240.16583265667803407No Hit
GACAAACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAA389790.14682880075988303No Hit
CTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAACCATGTCTGACAAACCCGATATGGCTG384190.14471935391862145No Hit
CACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTT371570.13996556478706415No Hit
CCGCAACCATGTCTGACAAACCCGATATGGCTGAGATCGAGAAATTCGAT350770.1321304765195212No Hit
CTTTTCCTCCGCAACCATGTCTGACAAACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGA330100.12434435755365039No Hit
GTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGT328150.12360981802856824No Hit
GTGGCTTTGAAGAACTTTGCCAAATACTTTCTTCACCAATCTCATGAGGAGAGGGAACATGCTGAGAAACTGATG325780.12271706999039146No Hit
CAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAG318060.11980904684493804No Hit
CAAACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAAAAATCC314370.11841907205132104No Hit
CCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAACCATGTCTGACAAACCCGATATGGCTG310850.11709313403681376No Hit
CCATGTCTGACAAACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCA293560.11058021691441869No Hit
CATGTCTGACAAACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAG283820.10691128615836733No Hit
CCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACA271220.1021650307655288No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCAA798550.027.82943267
CACGCGC84200.027.4857370
CTGCCTT816250.026.8406463
CGCCTAA25950.026.6157249
ACTGCCT829900.026.42077362
CCTTCCA875400.025.12287366
CTTGACG30350.024.8827131
ATCCACT887950.024.74709558
AACGATT289200.024.69086670
TGCCTTC898050.024.52789964
ACGTCTG55500.024.28144
GCCTTCC907300.024.23149765
AATCCAC915050.024.12489757
CATTCGG174150.023.6325234
TCCACTG930650.023.57405359
TGTAGGC47750.023.5053023
CACTGCC943550.023.30613961
TAGGCAG48900.022.6708645
GCATTCG184400.022.3740733
ACACGCG72750.022.17212569
Produced by FastQC (version 0.11.5)