FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005284478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5843638
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA2022983.4618503062646933No Hit
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT1139681.9502919243115333RNA PCR Primer, Index 3 (100% over 34bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA643901.1018820809913277RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG361640.6188610588130202RNA PCR Primer, Index 1 (100% over 29bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA302340.517383178081873RNA PCR Primer, Index 7 (97% over 35bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA218050.3731408413731309No Hit
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC202030.3457264122110233RNA PCR Primer, Index 1 (100% over 27bp)
TTCGACCTGAGCTCAGGCAAGGCTACCCTGGAATTCTCGGGTGCCAAGGA172940.29594577898220253RNA PCR Primer, Index 1 (100% over 22bp)
TTCGACCTGAGCTCAGGCAAGGCTACCCGTGGAATTCTCGGGTGCCAAGG130920.22403851847085668Illumina Small RNA Adapter 2 (100% over 21bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAG126730.2168683275726525RNA PCR Primer, Index 7 (97% over 36bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA120580.20634406169581346RNA PCR Primer, Index 1 (100% over 28bp)
TCTTAACCCGGACCAGAAACTATGGAATTCTCGGGTGCCAAGGAACTCCA117880.20172365228646952RNA PCR Primer, Index 1 (100% over 28bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG117380.20086802091436878No Hit
ATACGATGAAGAGTTTGATCCTGGCTCATGGAATTCTCGGGTGCCAAGGA114740.19635028726967688RNA PCR Primer, Index 1 (100% over 22bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC110920.18981326358682724RNA PCR Primer, Index 1 (100% over 33bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG109560.18748594625471326Illumina Small RNA Adapter 2 (100% over 21bp)
AGGTTCCGGATAAGTAAGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCA109290.18702390531377885RNA PCR Primer, Index 1 (100% over 28bp)
GTCCCACTCCGTAGATCTGTTCTGGAATTCTCGGGTGCCAAGGAACTCCA102710.17576379645693316RNA PCR Primer, Index 1 (100% over 28bp)
ATATACGGAGAGTTTGATTCTGGCTCTGGAATTCTCGGGTGCCAAGGAAC96820.1656844588935865RNA PCR Primer, Index 1 (100% over 24bp)
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA88820.1519943569399747RNA PCR Primer, Index 1 (100% over 23bp)
ATACGATGAAGAGTTTGATCCTGGCTCTGGAATTCTCGGGTGCCAAGGAA87240.1492905618041364RNA PCR Primer, Index 1 (100% over 23bp)
TTCGACCTGAGCTCAGGCAAGGCTACCCGCTTGGAATTCTCGGGTGCCAA84100.14391719678734377No Hit
ATATACGGAGAGTTTGATTCTGGCTCATGGAATTCTCGGGTGCCAAGGAA78980.13515553153703225RNA PCR Primer, Index 1 (100% over 23bp)
AAACTTGACCGTACTGTAAACCGCATCATGGAATTCTCGGGTGCCAAGGA78830.134898842125402RNA PCR Primer, Index 1 (100% over 22bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC78630.13455658957656172RNA PCR Primer, Index 1 (100% over 27bp)
GGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCAAGGA76400.13074047365699246RNA PCR Primer, Index 1 (100% over 22bp)
AAGATTAAGAGAACCAACACCTTGGAATTCTCGGGTGCCAAGGAACTCCA72180.12351894487646223RNA PCR Primer, Index 1 (100% over 28bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA71190.12182479475970277RNA PCR Primer, Index 1 (100% over 22bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC70670.12093493813271801RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGC65700.11242996229403669RNA PCR Primer, Index 10 (100% over 50bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA62560.10705659727724408RNA PCR Primer, Index 1 (100% over 32bp)
CGAACTGTAGAGTTTGATCCTGGCTCATGGAATTCTCGGGTGCCAAGGAA62530.10700525939491803RNA PCR Primer, Index 1 (100% over 23bp)
TTCGACCTGAGCTCAGGCAAGGCTACCTGGAATTCTCGGGTGCCAAGGAA60630.10375386018093523RNA PCR Primer, Index 1 (100% over 23bp)
GAGACGAAGCAGCTGAATCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT60450.10344583288697898RNA PCR Primer, Index 1 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTGGT256800.068.965441
TGCCCCG161550.068.7435714
TGGTCCG261650.068.716724
GCTGGTC259400.068.664982
GCCCCGA159550.068.2890815
ACTGAGC163700.068.143537
GCTGCCC163350.068.0074912
TGTACTG165250.067.7689744
CTGCCCC164600.067.76745613
CTGGTCC262850.067.737093
GTCTACG22850.067.104211
ATACGAT30900.067.0694961
CCCCGAG115900.067.0389716
ATATACG27000.066.644021
GAGCTGC168000.066.416810
CCATACC23400.066.410399
CACTCCG12600.066.402665
AGGCTAC63850.066.38058520
CCTGTAC170350.066.315492
CTGTACT168850.066.2618943
Produced by FastQC (version 0.11.5)