FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005284480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3978854
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA197352249.60026178392069No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGC1841104.627211754942503RNA PCR Primer, Index 9 (100% over 50bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA1445203.6322016339378123No Hit
TCTTAACCCGGACCAGAAACTATGGAATTCTCGGGTGCCAAGGAACTCCA1335873.3574240220927933RNA PCR Primer, Index 1 (100% over 28bp)
AGGTTCCGGATAAGTAAGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCA1212513.047385000806765RNA PCR Primer, Index 1 (100% over 28bp)
GTCCCACTCCGTAGATCTGTTCTGGAATTCTCGGGTGCCAAGGAACTCCA834502.0973375750907173RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGAATTCTCGGGTGCC789801.984993669031334No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACATGGAATTCTCGGGTGCCA229820.5776035009075478No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA213620.5368882597853553RNA PCR Primer, Index 1 (100% over 23bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG193700.4868235929239927No Hit
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA193270.4857428797337123RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTTGGAATTCTCGGGT145830.36651256869440296No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAT145630.3660099113965981RNA PCR Primer, Index 9 (100% over 36bp)
TGATACGGATGTTATACGCAGCTGGAATTCTCGGGTGCCAAGGAACTCCA110130.27678824103623806RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG106180.2668607594045924RNA PCR Primer, Index 1 (100% over 29bp)
TAGCATATCGAGCCTGAGAACATGGAATTCTCGGGTGCCAAGGAACTCCA106160.2668104936748119RNA PCR Primer, Index 1 (100% over 28bp)
CCTGGAACTTAGGACGTGAATCTGGAATTCTCGGGTGCCAAGGAACTCCA104900.2636437526986414RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATGTCGTATGC93010.2337607763441433RNA PCR Primer, Index 9 (98% over 50bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG90660.22785455309493638RNA PCR Primer, Index 6 (100% over 34bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC87680.22036495935764416RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA81450.20470718453102327RNA PCR Primer, Index 1 (100% over 28bp)
CCCCCCACTGCTAAATTTGACTGGCTTTTGGAATTCTCGGGTGCCAAGGA78820.19809724106488952RNA PCR Primer, Index 1 (100% over 22bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGAGCTCGTATGC78680.19774538095642616RNA PCR Primer, Index 9 (98% over 50bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC75580.18995419284045104RNA PCR Primer, Index 1 (100% over 26bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTATGGAATTCTCGGG72800.1829672564009637No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTATCGGGTGCCA57120.14355892425306382No Hit
TGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA55600.139738728789747No Hit
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC55440.13933660295150313RNA PCR Primer, Index 1 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATGGAATTCTCGGGTGC54290.13644632348912525No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGCTCTCGTATGC52920.13300312099916206RNA PCR Primer, Index 9 (98% over 50bp)
GGGTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA49470.12433228261202849No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATATCGTATGC44800.11259523470828535RNA PCR Primer, Index 9 (98% over 50bp)
GGATGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA43110.1083477805418344No Hit
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTTGGAATTCTCGGGT42510.10683980864841987No Hit
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA41930.10538210248478583RNA PCR Primer, Index 7 (97% over 35bp)
GCTCTACACTCTACTCGTCAGCTGTTGGAATTCTCGGGTGCCAAGGAACT41060.10319554323933475RNA PCR Primer, Index 1 (100% over 25bp)
TATGCATATGATCACGAGACTCAGTTGGAATTCTCGGGTGCCAAGGAACT41010.10306987891488353RNA PCR Primer, Index 1 (100% over 25bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC40650.1021650957788348RNA PCR Primer, Index 1 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG3350.070.012621
CCAGAGG150.002223717370.012621
GGAAGCA150.002223717370.012621
CCGCAGG207.9239326E-570.012621
GGAGGGA150.002223717370.012621
GTTTGTC207.92788E-570.0055856
GTTCTAG150.002224604470.0055852
CGCGGAG207.92788E-570.0055855
GCACGTC150.002224604470.0055852
TTCGTCG150.002224604470.0055856
CTACGAG252.840934E-670.0055853
CACTACG150.002224604470.0055855
TTTGCTC150.002224604470.0055853
CCGCGCG252.840934E-670.0055852
TTGCACG150.002224604470.0055854
CCGTTAC401.3460522E-1070.0055854
GGGATGT150.002224604470.0055852
GGTCCGC900.070.005585
CGCTGCT1800.070.005586
AGGGGCA5150.070.005583
Produced by FastQC (version 0.11.5)