FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005284484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4847469
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA102614421.16865523018301No Hit
TCTTAACCCGGACCAGAAACTATGGAATTCTCGGGTGCCAAGGAACTCCA3373056.9583735347250295RNA PCR Primer, Index 1 (100% over 28bp)
AGGTTCCGGATAAGTAAGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCA2849585.878490403961325RNA PCR Primer, Index 1 (100% over 28bp)
GTCCCACTCCGTAGATCTGTTCTGGAATTCTCGGGTGCCAAGGAACTCCA1759403.6295229531122324RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGATCTCGTATGC1588453.276864689593683RNA PCR Primer, Index 17 (100% over 50bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA1100392.2700299888457254No Hit
TAGCATATCGAGCCTGAGAACATGGAATTCTCGGGTGCCAAGGAACTCCA241820.4988582701611914RNA PCR Primer, Index 1 (100% over 28bp)
TGATACGGATGTTATACGCAGCTGGAATTCTCGGGTGCCAAGGAACTCCA218140.4500080351210085RNA PCR Primer, Index 1 (100% over 28bp)
CCTGGAACTTAGGACGTGAATCTGGAATTCTCGGGTGCCAAGGAACTCCA217610.4489146810428287RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA181250.37390646541525074RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG149900.309233540224806No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTA140000.2888105112172971RNA PCR Primer, Index 3 (97% over 36bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG137750.28416891371559055RNA PCR Primer, Index 6 (100% over 34bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT122210.2521109469704706No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC121750.25116199814789947No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGAATTCTCGGGTGCC114960.23715468835386055No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTTGGAATTCTCGGG114530.23626762749797883No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGATGTCGTATGC93790.19348241319335927RNA PCR Primer, Index 17 (98% over 50bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG93040.19193521402612373RNA PCR Primer, Index 1 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACATGGAATTCTCGGGTGCCA79040.16305416290439403No Hit
TGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA77950.16080556678134508No Hit
ATAGGCGATAGAAATTGAAACCTTGGAATTCTCGGGTGCCAAGGAACTCC70510.1454573510423687RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC68380.14106330540741985No Hit
GCTCTACACTCTACTCGTCAGCTGTTGGAATTCTCGGGTGCCAAGGAACT68150.14058883099613428RNA PCR Primer, Index 1 (100% over 25bp)
AGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA66900.13801016571740843No Hit
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC66160.1364835958724027RNA PCR Primer, Index 1 (100% over 27bp)
CGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA63250.13048046310352887No Hit
TGTAGTATTCTCTGATGGTAGTTTGTATTTCTGTGGAATTCTCGGGTGCC49720.10256899012660009No Hit
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT49510.10213577435977414RNA PCR Primer, Index 1 (97% over 35bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCT48520.10009347145902324No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTAG150.002259568569.7310641
TAGCCCG150.002262528869.7080464
AGCTACG353.8417056E-969.7080464
TTCGCCC150.002262528869.7080462
TCGACCG208.097062E-569.708044
CGGACCG401.4006218E-1069.701579
TGGTCCG1388150.069.539824
AGGTTCC323600.069.515581
GCTGGTC1369600.069.486642
GGTCCGA1396200.069.46345
GTCCGAT1396500.069.388576
GGCTGGT1344800.069.370691
TCCGATG1397200.069.359137
GGGTTAT1409650.069.31660519
AGTGGGT1405650.069.29462416
GTTATCA1403850.069.2341421
CTTAACC396350.069.233182
TGGGTTA1412000.069.2209918
GGTTCCG324650.069.2141952
GGTTATC1403500.069.21416520
Produced by FastQC (version 0.11.5)