FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005284485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2724510
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA1612695.9191928089821655RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA1381915.072141412584282No Hit
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG794062.914505727635428RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC421291.5462963982514286RNA PCR Primer, Index 1 (100% over 27bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA238780.8764144745293649RNA PCR Primer, Index 1 (100% over 34bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA222790.8177250221140683No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA168300.6177257561910214RNA PCR Primer, Index 1 (100% over 28bp)
TGCAATTCACATGTTACAAGGAAATCAAAACAACTAATAATCTCGTAACA126190.4631658536764409No Hit
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC98000.3596977071106364RNA PCR Primer, Index 1 (100% over 27bp)
TGCAATTCACATGTTACAAGGAAATCAAAACAACTAATACTCTCGTAACA80270.2946217852017427No Hit
TCCTGTACTGAGCTGCCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT74330.2728196996891184RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG63710.233840213469578RNA PCR Primer, Index 1 (100% over 29bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGC63200.23196831723869613RNA PCR Primer, Index 12 (100% over 50bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA62260.22851815555824717RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA58720.21552499348506704RNA PCR Primer, Index 1 (100% over 28bp)
AGGTTCCGGATAAGTAAGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCA57390.21064338174570843RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG54400.19966893129406754Illumina Small RNA Adapter 2 (100% over 21bp)
GTCCCACTCCGTAGATCTGTTCTGGAATTCTCGGGTGCCAAGGAACTCCA52720.19350268488645664RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGTATGC46520.17074629933455926RNA PCR Primer, Index 13 (100% over 50bp)
TCTTAACCCGGACCAGAAACTATGGAATTCTCGGGTGCCAAGGAACTCCA45200.16590139144286495RNA PCR Primer, Index 1 (100% over 28bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAG34540.12677508983266716RNA PCR Primer, Index 2 (97% over 35bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGC34320.12596760518405145No Hit
TGAGGGGCAGAGAGCGAGACTTGGAATTCTCGGGTGCCAAGGAACTCCAG33280.12215040502695897RNA PCR Primer, Index 1 (100% over 29bp)
GCTCTACACTCTACTCGTCAGCTGTTGGAATTCTCGGGTGCCAAGGAACT30300.11121265842298249RNA PCR Primer, Index 1 (100% over 25bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG29950.10992802375473021No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGAATTCTCGGGTGCC29430.10801942367618397No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29410.10794601598085526No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGT28260.10372507349945495RNA PCR Primer, Index 8 (97% over 36bp)
AAGCTGCCAGTTGAAGAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAG27690.10163295418258696RNA PCR Primer, Index 1 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGTTA39650.069.8025310
TGTTACA40000.069.6291312
GGCTGGT186100.069.6267241
GCTGGTC188500.069.421442
TGCCCCG340500.069.29053514
TACGGCC17950.069.214564
GCCCCGA332650.069.1796215
ACTGAGC343450.069.1334467
GCTGCCC341900.069.1193812
CCTGTAC346350.069.1178742
CCCCGAG240700.069.0935616
GAGCTGC344400.069.08498410
TCCTGTA342950.069.050211
TGTACTG343200.069.035894
CTGCCCC342200.068.9974413
GTACTGA344700.068.847155
CTGTACT345050.068.716463
GTCTACG18150.068.690081
TGAGCTG345550.068.59189
TGGTCCG192550.068.522214
Produced by FastQC (version 0.11.5)