FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005284487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27908031
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA1908130.6837207540725463No Hit
GTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGT1690110.6055998719508374No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCA939350.33658770122478365No Hit
GTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAG852700.30553929082277426No Hit
CCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACT847720.3037548582341764No Hit
GGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGT815670.29227070874330047No Hit
CAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAG768030.27520035361864115No Hit
CACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCA766970.27482053463391953No Hit
GTTTACCAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCT738230.2645224236708065No Hit
CTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAAAAATCCACTGCCTTCCAA736090.263755619305425No Hit
GGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACC726180.26020467011807463No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACAC723980.25941636656487876No Hit
CCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGAGGGTTC643230.2304820429646219No Hit
GCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGG640050.229342586010457No Hit
AATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATG613650.21988294337210676No Hit
CCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTC613110.21968945068177687No Hit
GTTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTT509770.18266068286938625No Hit
CCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTG489720.17547637094139676No Hit
TGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAA449290.16098950155243844No Hit
ACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTT447470.16033735952206732No Hit
GGCAAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACC436310.1563385105885829No Hit
CTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAAGACGAGAAGACCCTATGG426640.15287355815249024No Hit
CAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATA362920.1300414206935631No Hit
ATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGC348230.12477770287699624No Hit
CACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGC344760.12353433318172823No Hit
GGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCG341770.12246295698897568No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG338720.1213700816084087No Hit
CTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAA332710.11921657962899639No Hit
CTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGC331740.1188690094259964No Hit
GTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATA331730.11886542622802734No Hit
CCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCAT331570.11880809506052219No Hit
AATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAAGACGAGAAGAC328270.1176256397307284No Hit
CGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGG299290.1072415320163576No Hit
CAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCA292530.10481929018926488No Hit
AACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTTACCA286850.10278403374283193No Hit
TATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAA281740.10095301958063611No Hit
GTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAAGACGAGAAGACCCTATGGAGCTTTAATTTATT280610.10054811821013099No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGACG34100.038.048471
GTTAGGG120600.034.5474171
CGCCTAA37150.031.13438849
TAGGTCG16550.029.9745923
ACGTCTG33800.026.6864384
GTAGGGT11600.026.02240670
TCTAGGC67550.025.48807570
TGTAATC46500.025.00472570
CCGCCTA44700.024.70703548
TTGACGA48300.024.0603962
GCGTTCG50350.023.49804320
CGAGCTT48200.022.97275234
CGCGATT7100.022.83238870
ATAACGT20050.022.4928343
CGTTCGC53750.022.20614421
CGTTGCG4550.022.11237113
TTCGCAA54250.021.30949439
CCCCGTT42450.021.26818346
TAGCGAT8650.021.2625125
CATTCGG86150.021.2221454
Produced by FastQC (version 0.11.5)