FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005284503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5648804
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA1597042.8272179385229155No Hit
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA905891.6036846029708236RNA PCR Primer, Index 1 (100% over 28bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC379350.671558085569972RNA PCR Primer, Index 2 (100% over 34bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG367590.6507395193743667RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC314270.5563478569976936RNA PCR Primer, Index 1 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA210550.3727337680684265No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA145300.2572225908351573RNA PCR Primer, Index 1 (100% over 28bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG112520.19919260785114865No Hit
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC111020.19653717848946434RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC102210.18094095670517157RNA PCR Primer, Index 2 (100% over 50bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC98010.1735057544924554RNA PCR Primer, Index 1 (100% over 26bp)
AGGTTCCGGATAAGTAAGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCA87490.1548823432358425RNA PCR Primer, Index 1 (100% over 28bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC80460.1424372309607485RNA PCR Primer, Index 1 (100% over 31bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG79040.139923424498354Illumina Small RNA Adapter 2 (100% over 21bp)
GTGTTCGGAAGATTTTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC76880.13609960621752853RNA PCR Primer, Index 1 (100% over 33bp)
TCTTAACCCGGACCAGAAACTATGGAATTCTCGGGTGCCAAGGAACTCCA76440.13532068027143446RNA PCR Primer, Index 1 (100% over 28bp)
TTCGACCTGAGCTCAGGCAAGGCTACCCTGGAATTCTCGGGTGCCAAGGA74720.13227578793670305RNA PCR Primer, Index 1 (100% over 22bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGA73760.13057631314522508RNA PCR Primer, Index 2 (100% over 36bp)
GTCCCACTCCGTAGATCTGTTCTGGAATTCTCGGGTGCCAAGGAACTCCA72720.12873521545445726RNA PCR Primer, Index 1 (100% over 28bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG69350.1227693508218731RNA PCR Primer, Index 2 (100% over 35bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC67490.1194766184133845RNA PCR Primer, Index 1 (100% over 26bp)
GCTCTACACTCTACTCGTCAGCTGTTGGAATTCTCGGGTGCCAAGGAACT60980.10795205498367441RNA PCR Primer, Index 1 (100% over 25bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA57250.10134888730428601RNA PCR Primer, Index 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCCCG211600.068.9754214
GGCTGGT208450.068.629581
GCTGGTC209550.068.553282
ACTGAGC212400.068.468457
GCTGCCC212550.068.45306412
GCCCCGA210800.068.32278415
TGTACTG214500.068.114184
GTACTGA214850.068.003235
TACGGCC25750.067.968114
TACTGAG214400.067.8847356
ATTGCAC54050.067.610382
GGGTTAT215100.067.5746819
CCCCGAG165600.067.4206316
AGTGGGT214250.067.3853616
CTGCCCC218400.067.3566913
GTCCGAT213700.067.288116
GGTCCGA217450.067.1901255
GTCTACG25850.067.162941
CACTTGT52900.067.161876
TATTGCA50750.067.109711
Produced by FastQC (version 0.11.5)