FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005284505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34454628
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA452637613.13720757629425No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA17715445.141672114410871No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC8641672.5081304026849454RNA PCR Primer, Index 1 (100% over 27bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA7969732.3131087063253157RNA PCR Primer, Index 1 (100% over 28bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT6321121.8346214621733834RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTGCCAAGGAACTCC6002901.7422623166908087RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA5067221.4706935741694844RNA PCR Primer, Index 1 (100% over 28bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA4147511.2037599128918182RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT3808131.1052593573205898RNA PCR Primer, Index 1 (100% over 30bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA3803131.1038081734622123RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC2972750.8628013629983177RNA PCR Primer, Index 1 (100% over 26bp)
CCCATAAAGTAGAAAGCACTACTGGAATTCTCGGGTGCCAAGGAACTCCA2943990.8544541534449305RNA PCR Primer, Index 1 (100% over 28bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA2874060.8341578960016635RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC2552220.7407480934056232RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG2180790.6329454493021953RNA PCR Primer, Index 1 (100% over 29bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG1970820.5720044343534924RNA PCR Primer, Index 6 (100% over 34bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG1925790.558935072524945RNA PCR Primer, Index 1 (100% over 29bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA1685280.4891302265692725RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGGGCAGAGAGCGAGACTTGGAATTCTCGGGTGCCAAGGAACTCCAG1345470.3905048691862237RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA1293880.37553155413548506RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC1279330.37130860910760666RNA PCR Primer, Index 1 (100% over 27bp)
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG1115890.3238723111449643RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGGGCAGAGAGCGAGACTTTGGAATTCTCGGGTGCCAAGGAACTCCA1092600.31711269673264214RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAG1031000.29923411159743185No Hit
TGAGGGGCAGAGAGCGAGACTTTTTGGAATTCTCGGGTGCCAAGGAACTC1021470.2964681551633644RNA PCR Primer, Index 1 (100% over 26bp)
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTC970270.28160803245357924RNA PCR Primer, Index 1 (100% over 26bp)
AAGCTGCCAGTTGAAGAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAG963850.2797447123794226RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT909690.26402548882547794RNA PCR Primer, Index 1 (100% over 30bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG889160.25806692790298014RNA PCR Primer, Index 1 (100% over 29bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG822170.23862396656843893No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA816760.23705378563367452RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA779120.226129273547809RNA PCR Primer, Index 1 (100% over 28bp)
AACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA745230.2162931493557266RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC677110.19652222046919213RNA PCR Primer, Index 1 (100% over 27bp)
GGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCAAGGA667280.19366919300362204RNA PCR Primer, Index 1 (100% over 22bp)
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGTGCCAAGGAACTCC658270.19105415969082587RNA PCR Primer, Index 1 (100% over 27bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGGAATTCTCGGGTGCCAA653560.18968714449623428No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA635020.18430615474937068RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC619310.1797465350663487RNA PCR Primer, Index 1 (100% over 26bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA600150.1741855985210463No Hit
CATTGCACTTGTCTCGGTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT595450.17282148569417147RNA PCR Primer, Index 1 (100% over 30bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA591180.17158217467911713RNA PCR Primer, Index 1 (100% over 28bp)
GGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAAGGAA578890.16801516475522532RNA PCR Primer, Index 1 (100% over 23bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC561190.16287797389656913RNA PCR Primer, Index 1 (100% over 31bp)
CCCATAAAGTAGAAAGCACTATGGAATTCTCGGGTGCCAAGGAACTCCAG544690.1580890671639235RNA PCR Primer, Index 1 (100% over 29bp)
TGTCAGTTTGTCAAATACCCCATGGAATTCTCGGGTGCCAAGGAACTCCA533730.15490807214636013RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGAAGGTAGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCC524180.1521363109768592RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA520260.15099858283189124RNA PCR Primer, Index 1 (100% over 28bp)
TGTCAGTTTGTCAAATACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC514670.14937615927822526RNA PCR Primer, Index 1 (100% over 31bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC511420.1484328897702799RNA PCR Primer, Index 1 (100% over 26bp)
GGCTGGTCCGATGGTAGTGGGTTATCTGGAATTCTCGGGTGCCAAGGAAC478160.13877961474435307RNA PCR Primer, Index 1 (100% over 24bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG474100.13760125345135057Illumina Small RNA Adapter 2 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTTGGAATTCTCGGGTGCCAAGGAACTCC463490.1345218413038736RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCATGGAATTCTCGGGTGCCAAGGAACTCCA462330.13418516664873004RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT437860.12708307284583076RNA PCR Primer, Index 1 (100% over 30bp)
TGTCAGTTTGTCAAATACCCCTGGAATTCTCGGGTGCCAAGGAACTCCAG437140.1268741023702244RNA PCR Primer, Index 1 (100% over 29bp)
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTCC428250.12429389747002928RNA PCR Primer, Index 1 (100% over 27bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTTGGAATTCTCGGGTGCCAAGGAA422510.12262793840061195RNA PCR Primer, Index 1 (100% over 23bp)
GTCAGGATGGCCGAGCGGTCTAAGGCTGGAATTCTCGGGTGCCAAGGAAC420590.12207068379899502RNA PCR Primer, Index 1 (100% over 24bp)
TGTCAGTTTGTCAAATACCCCAATGGAATTCTCGGGTGCCAAGGAACTCC417970.12131026345720522RNA PCR Primer, Index 1 (100% over 27bp)
ACCACAGGGTAGAACCACGGACTGGAATTCTCGGGTGCCAAGGAACTCCA408440.1185443070231378RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGAATTCTCGGGTGCC396000.11493376158349466No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA394530.1145071135291317RNA PCR Primer, Index 1 (100% over 28bp)
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAATGGAATTCTCGGGTGCCAA393550.11422268149288972No Hit
AAACCGTTACCATTACTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG387900.1125828437329232RNA PCR Primer, Index 1 (100% over 29bp)
AGTTGGTCCGAGTGTTGTGGGTTATTGTTGGAATTCTCGGGTGCCAAGGA380510.1104379939902413RNA PCR Primer, Index 1 (100% over 22bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT368940.10707995454195587RNA PCR Primer, Index 1 (97% over 35bp)
TGTCAGTTTGTCAAATACCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT357450.10374513403540446RNA PCR Primer, Index 1 (100% over 30bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA356690.10352455408893109RNA PCR Primer, Index 1 (100% over 32bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC356160.1033707285999431RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCTGGAATTCTCGGGTGCCAAGGAA350330.10167864822107499RNA PCR Primer, Index 1 (100% over 23bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTG350020.10158867482185559RNA PCR Primer, Index 15 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTGGT7813050.069.838061
TGGTCCG8038050.069.785234
GCTGGTC7838650.069.7512
CTGGTCC7834450.069.636123
GGGCAGA1235350.069.596545
GAGGGGC1237200.069.559982
GGGGCAG1240100.069.5472264
AGAGAGC1228450.069.521149
CAGTTGA1076000.069.5146268
AGGGGCA1238450.069.492693
TGAGGGG1209050.069.4684751
GCAGAGA1234600.069.421357
AGCGAGA1224550.069.4191813
TCAGTGC524750.069.359041
GAGCGAG1230050.069.3563312
CCCATAA1284100.069.350351
GAGAGCG1227500.069.3267810
AAGCTGC1053100.069.274851
GCGAGAC1220750.069.2680914
AGAGCGA1229100.069.2505711
Produced by FastQC (version 0.11.5)