FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005284506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3700316
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA2373406.414046800327323RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA1671864.518154665709631No Hit
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG1159573.1337053375981943RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC692851.8724076538328078RNA PCR Primer, Index 1 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA311720.8424145397312014No Hit
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC304430.8227135196021097RNA PCR Primer, Index 2 (100% over 34bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA273460.7390179649521824RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC156540.42304495075555715RNA PCR Primer, Index 1 (100% over 27bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA102920.27813840763869896RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG96390.2604912661513233RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT93860.2536540122519266RNA PCR Primer, Index 1 (100% over 30bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTATGC86820.23462861009708358RNA PCR Primer, Index 16 (100% over 50bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG69950.18903790919478228Illumina Small RNA Adapter 2 (100% over 21bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA66230.17898471373796182RNA PCR Primer, Index 1 (100% over 28bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCG63500.17160696545916618RNA PCR Primer, Index 7 (97% over 36bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCC63280.17101242164182734RNA PCR Primer, Index 5 (97% over 35bp)
GTCCCACTCCGTAGATCTGTTCTGGAATTCTCGGGTGCCAAGGAACTCCA55590.15023041275393778RNA PCR Primer, Index 1 (100% over 28bp)
AGGTTCCGGATAAGTAAGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCA53590.1448254689599483RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACAGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA52450.1417446509973743RNA PCR Primer, Index 1 (100% over 28bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC51170.13828548696922102RNA PCR Primer, Index 1 (100% over 31bp)
TCTTAACCCGGACCAGAAACTATGGAATTCTCGGGTGCCAAGGAACTCCA48030.12979972521265754RNA PCR Primer, Index 1 (100% over 28bp)
TTCGACCTGAGCTCAGGCAAGGCTACCCTGGAATTCTCGGGTGCCAAGGA44310.11974652975583708RNA PCR Primer, Index 1 (100% over 22bp)
TGAGGGGCAGAGAGCGAGACTTGGAATTCTCGGGTGCCAAGGAACTCCAG42150.11390919045832842RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA41910.11326059720304969RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACAGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG41330.11169316350279274RNA PCR Primer, Index 1 (100% over 29bp)
GTGTTCGGAAGATTTTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC37900.10242368489610076RNA PCR Primer, Index 1 (100% over 33bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA37100.10026170737850496RNA PCR Primer, Index 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTGGT217800.069.381621
GCTGGTC218150.069.363912
CCTGTAC526000.069.1406252
TCCTGTA523150.069.0876851
TGCCCCG525300.069.07017514
GCCCCGA518800.068.9776115
ACTGAGC506750.068.946497
CCCCGAG380300.068.8919216
GCTGCCC526800.068.8858612
TACGGCC20800.068.8258744
TATTGCA56500.068.785271
TGTACTG503750.068.773984
CTGCCCC528450.068.7303813
CTGTACT503950.068.719833
TGGTCCG222100.068.711544
GTACTGA506900.068.665175
GAGCTGC530550.068.6505510
CCCGAGA84900.068.42979417
TGAGCTG508400.068.426719
CTGGTCC221550.068.377493
Produced by FastQC (version 0.11.5)