FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005284514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24002759
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGC5270012.195585099196305No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC4146831.7276472258876574No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3535911.4731264851678092No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGC3373261.4053634417610075No Hit
GCCTACAGCACCCGGTATTCCCAGGCGGTCTCCCATCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCG1774980.7394899894632946No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT1564470.651787571587083TruSeq Adapter, Index 3 (97% over 36bp)
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAA1516690.6318815266195024No Hit
GCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCG1441260.6004559725821519No Hit
CCTACATATAGTTATAGAATAACGAAGATTCGGCAGGGTAGAGAGTATGT1122120.4674962574094086No Hit
GTCTAAATTGACCCTGACAACTTACAGTGCGTCTAGGTCGGGGCAGGAAG983960.40993620775011735No Hit
CGACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCG867840.3615584358448127No Hit
GCTAATTTATATACGTCACGGCTTCTATTCGATGATCCGATATGGTAAAATTTATTCAGACTGTAAAGTAGGACC846630.35272195167230563No Hit
CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAATGTGTCCTGC825170.3437813128065819No Hit
AGAGTATGGTTTAGGGCCCTGGATCGTGCGAGAGAAAGAAAACCATGGGA785890.32741652740837No Hit
GGCTTCTATTCGATGATCCGATATGGTAAAATTTATTCAGACTGTAAAGT722340.30094040439267833No Hit
CGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTC704910.29367873918160825No Hit
AGCCAGGCTATCTAAATGGTTTTAAGCGTGGCCCGTATCTGCAGGGGCGA697020.2903916170636884No Hit
ACCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGA676320.28176760846534354No Hit
GACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGT668420.278476320159695No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT611270.2546665572903515No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCGCGTAT607800.25322089014850335TruSeq Adapter, Index 3 (97% over 36bp)
GGGAATGTTCGCGAATATATGCTGAAGCCGTGTCTCTGTGGACGGGAAAGGGCGAATCATAGGGAGGTGAAGGG583020.2428970769568615No Hit
GCTGTATCGATCAGTCCATGTTGCATTACCAGCCTAAAATTGCCAAATAT577480.24058900895517887No Hit
GCCCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGGGAATGTTCGCGAATATATGCTGA556690.23192750466727594No Hit
CCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGG545690.22734469816574004No Hit
GGTTGATCTATTGCGTCCCTTGCACACTCTTACTTCGAGCTGGTGTACTTGGTGACCGCCTTGGTGCCCTCGGAC545250.22716138590567858No Hit
CCTAAATCAAGACACAGCACCCTTTAATTATACAGACGATGGACAACAATAAAGTGTACAGACCACAGCTCAAAA544060.22666560956596699No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTAC531580.22146620728058802No Hit
GTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGG524910.21868736006556577No Hit
GTTGTGTCTCTGTGGACGGGAAAGGGCGAATCATAGGGAGGTGAAGGGAGGTGGGGTATAAGGGGAGTGGTGTCT521650.21732918286601968No Hit
GTATGGTTTAGGGCCCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGGGAATGTTCGCG510450.2126630526099104No Hit
GGCTAATTTATATACGTCACGGCTTCTATTCGATGATCCGATATGGTAAA502920.2095259132502226No Hit
AGCCCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGGGAATGTTCGCGAATATATGCTG496470.20683872216523108No Hit
GTGGCTTATGCTTACTGGTAAAAAGACTTTGTAATGAGTAAAAAAATTCA489000.20372657993191534No Hit
GCCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGA455380.18971985678813008No Hit
GGAAAGGGCGAATCATAGGGAGGTGAAGGGAGGTGGGGTATAAGGGGAGT452090.18834918102539794No Hit
GAAGCCGTGTCTCTGTGGACGGGAAAGGGCGAATCATAGGGAGGTGAAGG442110.18419132567218627No Hit
GTCTAGGTCGGGGCAGGAAGGAAGTTACTAGGCTAATTTATATACGTCAC427820.17823784340791823No Hit
GCGACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGC426480.17767957425227657No Hit
GGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTC419850.17491739178816904No Hit
GATTCGGCAGGGTAGAGAGTATGTTAATTGGGAAATGAAGCAATAATGATGTGGCTTATGCTTACTGGTAAAA411620.17148861928747441No Hit
AGCAATACAGAGTATGGTTTAGGGCCCTGGATCGTGCGAGAGAAAGAAAA405800.16906389802938904No Hit
GCTCAGACAGGCGTAGCCCCGGGAGGACCCCGGGGCCGCAAGTGCGTTCG395650.16483521748478996No Hit
CTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACT392580.16355619785208858No Hit
CTCCGTTCTTTTGTTACCACCAACCTTATTGCAGATCGCGCAAACCCAAATGCGTCGGCCCCCTTACCTCACAG386680.16109814709217388No Hit
GCTCCGTTCCGCTGTGGGGGCTCTGGACGTAACCGCTATAATGGTGCTGG385010.16040239374148615No Hit
CGCGTCTAGGTCGGGGCAGGAAGGAAGTTACTAGGCTAATTTATATACGTCACGGCTTCTATTCGATGATCCGAT378850.15783602210062603No Hit
GTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCTGCGTTTGTATAGCCGACAAGCGCAATTTGAAGCAC377040.1570819421217369No Hit
GTAAGAGATAATTTAATAGTACGAGGGCGTTCATGATTCAAGCGTCTAAGAGAGTAATGAGGATGCAAGGGTG376030.15666115716114135No Hit
ATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGC374570.15605289375275566No Hit
CGGTGCGTCTAGGTCGGGGCAGGAAGGAAGTTACTAGGCTAATTTATATA374290.15593624049635293No Hit
GTGAAGTTTGAGAAAACCTTCTAAAACACACCTGGATCGTGCGAGAGAAA373820.1557404296731055No Hit
GGGGGCTCTGGACGTAACCGCTATAATGGTGCTGGGCGTGTGTTGAACCC368760.15363233868239898No Hit
CACGGCTTCTATTCGATGATCCGATATGGTAAAATTTATTCAGACTGTAAAGTAGGACCATAGGGGAATTAAGGA368510.15352818398918225No Hit
AGGAGGTGAAGGGAGGTGGGGTATAAGGGGAGTGGTGTCTAAATTGACCC363780.15155757719352178No Hit
GTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGTTTCGTTTCCCCGGGACCTCTTGCCCC360050.15000358917072826No Hit
AAGGGAGGTGGGGTATAAGGGGAGTGGTGTCTAAATTGACCCTGACAACTTACAGTGCGTCTAGGTCGGGGCAGG354830.14782883917636302No Hit
TCTGGCTGTATCGATCAGTCCATGTTGCATTACCAGCCTAAAATTGCCAAATATGATATAGGATGACATTCCTAA343370.1430543880393083No Hit
GCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGGGAATGTTCGC339610.14148790145332876No Hit
GGGGCCCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAA338630.1410796150559192No Hit
GTAGAGTCATTTAGAATTAATCGAGTCAAGAAATAGCGTAGCACCATTATCATCATAAGTAAACAAAAGGCGTC335130.13962144935088502No Hit
GTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGG334920.13953395940858299No Hit
CTAAGGAGCTGCACAAGCTCGCGCACAGACCTACAGCGGGGAGAACAAGC334140.1392089967657468No Hit
ATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCATGA333480.13893402837565463No Hit
GTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAG332620.13857573623098912No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGC328790.13698008633090888No Hit
CCTACAGCACCCGGTATTCCCAGGCGGTCTCCCATCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCG325430.13558024725407608No Hit
GCTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGC324400.13515112991802317No Hit
GAGTCTTTTTATATTTAACCTAAACCAAATAACACTAATAGAGAAGAACTAATTATAGATGTAAAGTATCTGTAA313600.13065164717106062No Hit
GGGGCGTTCATGATTCAAGCGTCTAAGAGAGTAATGAGGATGCAAGGGTGAGAGAAACATTAGCACATGTGTGCA312490.13018920033317838No Hit
CGCCTCTTTGCATCATTCTCGGGTTTCCTTAACTTATTTCTGTGAGCGTTAGAGTAGAAGTGTGCTGTTGATGTC310890.12952261029659132No Hit
ACCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGA310160.1292184785923985No Hit
CGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGC309250.12883935550908962No Hit
TACACGAAGCGCTTAGAACACGCGTAGAGTCATTTAGAATTAATCGAGTC306470.1276811553205196No Hit
GAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAG305010.12707289191213395No Hit
CGGTAGAAAAATAAATAGTCGACGGGGAACGCGGTCATCCCACCTCCTAC304620.12691041059071584No Hit
AGACCAATGGTAAACAGCAAAGGACGGAAATACAAAAAGAGCCCAATAGACACAAAATTTGTACACGAAGCGCTT303520.12645212994056224No Hit
CAGGGTTTCTCCTCCAGCATCTTCCACATTCACCTTGCCCCACAGGCTTG301660.1256772190230298No Hit
GTCCGTTTTTCTATTTCGTAATTCTCTTCGTTTCCTTTGTCCTATTTCAT296990.12373160935374138No Hit
CAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATC291170.121306888095656No Hit
GCCAAATATGATATAGGATGACATTCCTAAAATGGAGCGGTGAACCGTCAAAGCCAGGCTATCTAAATGGTTTTA290970.12122356434108263No Hit
ATCGGGTCCAGCGTGGCAAACAGGAGGTCTTCTTCATAGCTGTCAGCACT290460.1210110887669205No Hit
GTCACCGCTGCGCAAGCCTGGGGGGGCATCCGTCTGACGCCAGGCCTCCA289720.12070279087499901No Hit
GTTTATTACAACAAAACAGCTCTTCTTTTTCCCTTGTCTCTCTTCAAAATCAAGACGTGATAATCGGAGTCCCC287860.11992787995746657No Hit
CACACATAACATAACAGATCCTAAATCAAGACACAGCACCCTTTAATTAT287430.1197487338851338No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA287070.1195987511269017No Hit
GTAAGTGTTCTTTACCTTCTCTTGAATAAATTCCTAAGGATGGTGGTGAT280340.11679490678550745No Hit
AGATTCTGGCTGTATCGATCAGTCCATGTTGCATTACCAGCCTAAAATTG278670.11609915343481973No Hit
CAGGGATCCCGAGTCCTCTGTAGGTAAATTCCCTGATCGTCTGATAATCA276950.11538256914548864No Hit
ATCCGATATGGTAAAATTTATTCAGACTGTAAAGTAGGACCATAGGGGAATTAAGGAAGGGAGGAAACCATGGA276180.11506177269038113No Hit
CTCTTTTGCCAACTTGGGAATGTTCGCGAATATATGCTGAAGCCGTGTCTCTGTGGACGGGAAAGGGCGAATCAT275480.11477013954937432No Hit
GGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAG274870.11451600209792549No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAACGAT269480.11227042691217287No Hit
CCACGATGTACAAACGTGCGTACCCAAAACTACCTAGGCAGAATTGTATCGGCAAAACCGATGGCCACGGTGCAT266920.11120388285363363No Hit
GGCAGGAAGGAAGTTACTAGGCTAATTTATATACGTCACGGCTTCTATTC266010.11082475977032473No Hit
GGAGATTCTGGCTGTATCGATCAGTCCATGTTGCATTACCAGCCTAAAAT265330.11054145900477524No Hit
CTCCAGCATCTTCCACATTCACCTTGCCCCACAGGCTTGTGATAGTAGCC262390.10931659981254654No Hit
GTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGC262240.1092541069966165No Hit
TGTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGT261990.1091499523033998No Hit
GGGGATTCGGCGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAA261440.108920811978323No Hit
CGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATT261140.10879582634646293No Hit
CCCGGATGGGGGTAAGAAGAGAAGGAAAAAACGAAAGACAGACAATCAAA260760.1086375112127735No Hit
AGGACGGCTTCGGGGCCCACGCCAGTGTCCCTCCGCGCGACCTGATGCAG260150.10838337376132469No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG256700.10694603899493388No Hit
CGACGCTCAGACAGGCGTAGCCCCGGGAGGACCCCGGGGCCGCAAGTGCG256380.10681272098761647No Hit
CCTGGCTGTATCGATCAGTCCATGTTGCATTACCAGCCTAAAATTGCCAAATATGATATAGGATGACATTCCTAA255840.10658774685026833No Hit
GTGCGTAATGAAGAACGGAGCAAGTACTTGTCGAGGGGTCCTGCATTCAAATAGGGGAAGGATCGATAGCACGG254190.10590032587503793No Hit
GTCGATCGATCTAATCCTTGGGGGTCTTCTTAGGGCGCGACGTCGAGGCC253040.10542121428624102No Hit
AGGCATTTCGTCAGGACCATTCCTGGAGGTAGACCCATAGGGATGAATCC252970.10539205097214031No Hit
CACTGTAGAGAAGCTATTCGATGTCCTACATATAGTTATAGAATAACGAAGATTCGGCAGGGTAGAGAGTATGTT252230.10508375308021882No Hit
GTCTTTTTATATTTAACCTAAACCAAATAACACTAATAGAGAAGAACTAATTATAGATGTAAAGTATCTGTAAAT249670.10401720902167955No Hit
CTCGGTTTAATTTTGTTCGATCCTATTTATTGTGAACAACATGGGTTGCT247740.10321313479004643No Hit
GCCGTCTGGATCTCCCTGGAGGTGATGGTCGAGCGCTTGTTGTAATGCGC246950.10288400595948156No Hit
CTCAGACAGGCGTAGCCCCGGGAGGACCCCGGGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAATGTGTCCTGC244920.10203826985056177No Hit
GGCACGTTCATCACACTCAGAATGTAGTCCACTCTGAACTGGTTCTCGGGGTTGGCCAGCTCCACGGGGGGCACC244790.10198410941008905No Hit
CGCCAGGCTATCTAAATGGTTTTAAGCGTGGCCCGTATCTGCAGGGGCGACACGCCTGGATCGTGCGAGAGAA244500.10186328996595767No Hit
CAGGGATATCCGCTGGTTAACTCATCCATTATGATCCGAGTCACCGACGC244440.10183829283958565No Hit
AGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGGGAATGTTCG243220.10133001793668803No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)