FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005284515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24002759
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTT4574481.905814244104188No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACC2846031.1857095261423904No Hit
AGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTG1567360.6529915998406683No Hit
AGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTC1518640.6326939332265928No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1491510.6213910659187137No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA1086210.45253547727575816No Hit
CGATGAAGAACGCAGCTAGCTGCGAGAATTAATGTGAATTGCAGGACACA989010.41204013255309524No Hit
GATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGT951440.3963877652564857No Hit
GGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAG849060.35373433529037224No Hit
GAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTA821920.3424273017947645No Hit
GTGATTTTTTGAGCTGTGGTCTGTACACTTTATTGTTGTCCATCGTCTGT764280.3184133957267163No Hit
CTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAATGTG719100.2995905595685896No Hit
ATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCA717340.29885731052834386No Hit
CAAAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTCCATGGTTTCCTCCCTTCCTTA685610.285637996865277No Hit
CCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTTAATTCCCCTATGGTCCTACTTTACAGTCTGAAT683180.2846256132472105No Hit
AAAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTTA675240.28131766019064725No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT673610.2806385715908742No Hit
GTTTCTCTCACCCTTGCATCCTCATTACTCTCTTAGACGCTTGAATCATG668500.2785096496615243No Hit
TTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA638780.26612773973192No Hit
GGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGCGTT626470.26099916263792844No Hit
CACTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAATG626140.2608616784428823No Hit
ACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTAGTACTTGGATGGG603330.25135860423378825No Hit
AGAGACACGGCTTCAGCATATATTCGCGAACATTCCCAAGTTGGCAAAAGAGTCCCATGGTTTTCTTTCTCTCGC600830.250317057301621No Hit
CAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTTAATTCCCCTATGGTCCTACTTTACAGTCTGAATAA575890.2399265851063205No Hit
ATTTAGACACCACTCCCCTTATACCCCACCTCCCTTCACCTCCCTATGAT573500.2389308662391686No Hit
CCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCA573370.2388767057986959No Hit
CTTTCAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGA561960.23412308560028453No Hit
GTCAATTTAGACACCACTCCCCTTATACCCCACCTCCCTTCACCTCCCTATGATTCGCCCTTTCCCGTCCACAG559860.233248186177264No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT547250.2279946234514124No Hit
AGCGAAATCCGCTCCTGCTCCCAAGAAGGGCTCCAAAAAGGCTGTTACGAAAGTGCAGAAGAAGGACGGCAAGA545440.22724054347252332No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA524310.21843738880184563No Hit
GTAAGTTGTCAGGGTCAATTTAGACACCACTCCCCTTATACCCCACCTCC524200.2183915607368303No Hit
CCACCATCCTTAGGAATTTATTCAAGAGAAGGTAAAGAACACTTGCACAC514130.2141962096940606No Hit
ATTTTACCATATCGGATCATCGAATAGAAGCCGTGACGTATATAAATTAG506810.21114656027667486No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAA505100.21043414217507247No Hit
GAACGAGAGAAGGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCA495600.20647626383283688No Hit
AAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCC488190.20338911872589313No Hit
GAACGCAGCTAGCTGCGAGAATTAATGTGAATTGCAGGACACATTGATCA487850.20324746834311838No Hit
AGAACACTTGCACACATGTGCTAATGTTTCTCTCACCCTTGCATCCTCATTACTCTCTTAGACGCTTGAATCATG483790.20155599612527875No Hit
GGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGA482870.2011727068542412No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCA469690.19568167142785545No Hit
TGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGC444920.18536202442394226No Hit
CTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTT439090.1829331369781282No Hit
GGGCAAGCTAAGCTCGATCGCCCAAAGCGGCGGCTAACCATAACTACGGATCCCCGCAGGCCACTTGTAGTTCCC431990.17997514369077322No Hit
GCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTAGTACTTGG408200.17006378308426962No Hit
CACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTAGTACTTGGATGGG406870.16950968011635661No Hit
CTCCCTATGATTCGCCCTTTCCCGTCCACAGAGACACGGCTTCAGCATATATTCGCGAACATTCCCAAGTTGGCA400300.16677249477862108No Hit
CTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCAC394920.16453108578059714No Hit
TGAAGAACGCAGCTAGCTGCGAGAATTAATGTGAATTGCAGGACACATTG382040.15916503598607143No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGA368720.1536156739314843No Hit
AGCCTACGAACAGTCAACACAAAAGCGCAAGTCGTGCCCTACACAAACTA366980.1528907572666959No Hit
CCTCCCTATGATTCGCCCTTTCCCGTCCACAGAGACACGGCTTCAGCATATATTCGCGAACATTCCCAAGTTGGC366820.15282409826303717No Hit
GAAGAACGCAGCTAGCTGCGAGAATTAATGTGAATTGCAGGACACATTGA363280.15134926780708835No Hit
TGACGATACGTTTTTACGCTCAACGCGACCACGTAGTCCCTCAAGGTCTA357260.1488412227944296No Hit
ATCGGATCATCGAATAGAAGCCGTGACGTATATAAATTAGCCTAGTAACT339300.14135874963374No Hit
GTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGA337910.14077964953945502No Hit
AGTCAAGCTATTTCACTGTCGTTTTTACCGAGAGCCTAGGGTACAGGGACTTGGAATCTTGCTATTCTGCTGTCA337890.14077131716399768No Hit
AGATTGTCTCGTACGGTTAAGAGCCTCCGCCCGTCTCTGGGACTATGGAC330050.13750502598472117No Hit
CAATAACGTCTCTTTCAGAAGGCATTGGTATCTTTTCCCCACTTCCAAGC327570.13647181142801126No Hit
AGACGATCAGGGAATTTACCTACAGAGGACTCGGGATCCCTGGGCCACTG323060.13459286076238153No Hit
CCTTCACCTCCCTATGATTCGCCCTTTCCCGTCCACAGAGACACGGCTTCAGCATATATTCGCGAACATTCCCA320240.13341799582289687No Hit
ATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGT318960.13288472379362723No Hit
CGTCAAGCACCGAAATAATATCGTTAGCGCCTCGATCCTGGAGAATGACGATACGTTTTTACGCTCAACGCGACC317430.13224729707114086No Hit
AGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAA316450.1318390106737313No Hit
CTCACTTCTTCTAAAAGCGAGCCCCGTCAAGCACCGAAATAATATCGTTA316310.13178068404552992No Hit
CGAATAGAAGCCGTGACGTATATAAATTAGCCTAGTAACTTCCTTCCTGC314410.13098910837708283No Hit
AATTTATTCAAGAGAAGGTAAAGAACACTTGCACACATGTGCTAATGTTT313550.13063081623241726No Hit
ATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAA312770.1303058535895811No Hit
GCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGG310270.12926430665741384No Hit
TGCAAAGGCTGTCTATTCCGTGCGTCTGACTTTATACACAATTACCTGTG304470.1268479177747858No Hit
CAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTT303330.12637297237371753No Hit
CCCACCTCCCTTCACCTCCCTATGATTCGCCCTTTCCCGTCCACAGAGACACGGCTTCAGCATATATTCGCGAAC299730.12487314479139669No Hit
CTGTACACCGTATACCTGGCCTAGTCTTTCCCGGGGCGGGTGTGAAGAGT299150.12463150590313389No Hit
TTCAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGA298870.12451485264673115No Hit
TCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAA297910.12411489862477892No Hit
AACGTCTGAGGTTATCAATAAGCTCCTAGTCCAGACGCCATGGGTCATTTCACAGAGGAGGACAAGGCTACTATC297720.1240357410579342No Hit
CGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAG296590.1235649618445946No Hit
CTTTACAGTCTGAATAAATTTTACCATATCGGATCATCGAATAGAAGCCG292990.12206513426227376No Hit
AGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGT292640.12191931769177035No Hit
TGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGG292280.12176933493353827No Hit
GGCTGTGCGAGGGGGGCTAGCTCCCAGGTCTTCAACCGTACGACGAAGACAGGAACTGGCGTTCTAACGCCGGGG290910.1211985672147106No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG287500.11977789719923447No Hit
GTAACGCAGTCCGGTACGTGGCGTCATGATGCATCGCCTCCCACGTCCAG284830.11866552507567983No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTCCTCCTGAGCGTGAAGCCG283560.11813641923413888No Hit
CCAGAATCTGTGTTTTAGAAGGTTTTCTCAAACTTCACTAGTGCTGTGTT282490.11769063714717129No Hit
CGCAGCTAGCTGCGAGAATTAATGTGAATTGCAGGACACATTGATCATCG281710.11736567450433512No Hit
AACGCAGCTAGCTGCGAGAATTAATGTGAATTGCAGGACACATTGATCAT280620.1169115600419102No Hit
GGGGAAACGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCATTCA280510.11686573197689483No Hit
CTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTCCTCCTGAGCGTGA276150.11504927412719512No Hit
TGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTG275320.1147034805457156No Hit
AGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAG274780.11447850640836747No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT273310.11386607681225312No Hit
ATAAATTAGCCTAGTAACTTCCTTCCTGCCCCGACCTAGACGCACTGTAA273030.11374942355585038No Hit
CTCTGCCAGGGCAGAGGGATTCGCCTATATACTGAATACCCACCATGCCTGTATGAGCCTACGAACAGTCAACAC272930.11370776167856371No Hit
GAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTT269000.11207044990119677No Hit
AGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCACAC266710.11111639291133156No Hit
ACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTG266140.11087892021079743No Hit
GTTTGCGCGATCTGCAATAAGGTTGGTGGTAACAAAAGAACGGAGACTAC265980.11081226120713873No Hit
ATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGT263740.10987903515591686No Hit
ATGGACTGATCGATACAGCCAGAATCTCCCTCGTCTTAATCTTTCGCGATCTGTGTGGACATGACTGCCTCAA263720.10987070278045952No Hit
GGAACGTCTGAGGTTATCAATAAGCTCCTAGTCCAGACGCCATGGGTCAT263310.10969988908358409No Hit
AAAACGAATAGCTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCC261210.1088249896605636No Hit
CACCACTCCCCTTATACCCCACCTCCCTTCACCTCCCTATGATTCGCCCTTTCCCGTCCACAGAGACACGGCTTC258960.10788759742161307No Hit
GATGAAGAACGCAGCTAGCTGCGAGAATTAATGTGAATTGCAGGACACAT258680.10777094416521034No Hit
CCCTAAGAAGACCCCCAAGGATTAGATCGATCGACCGGATCTTTCCACGA258290.10760846284379225No Hit
CGAGAGAAGGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCA255910.10661691016436903No Hit
GGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCACTGCCGCGTCT253460.1055961941708451No Hit
AGGGATCAACCTTTCCCGGCAAGGCGGAGGAATTGGGGCAAGAGGTCCCG252630.10525040058936559No Hit
CTATGATTCGCCCTTTCCCGTCCACAGAGACACGGCTTCAGCATATATTCGCGAACATTCCCAAGTTGGCAAA251360.10472129474782461No Hit
CAGTCAACACAAAAGCGCAAGTCGTGCCCTACACAAACTAAGGCGTCGGTGACTCGGATCATAATGGATGAGTTA250220.10424634934675635No Hit
GTAAAGAACACTTGCACACATGTGCTAATGTTTCTCTCACCCTTGCATCC248070.10335061898509251No Hit
AGTGCCTATTCGTTATTCGACAATCACCCAAACGTGAGCGTGCTCGACGC247730.10320896860231776No Hit
CAGATCCTCTGTCGACCAAACATGCTCATTCGAAACACCGCGCCAACCTATGGGGTTAGCAGTTTCGTGATCCT247310.10303398871771366No Hit
GGACTTTCTAGCTTAAGGAAATTGAAAACTGGTGCAATCGCTCTTTATAG245980.10247988574980067No Hit
GAGAGCTACTCCGTATACGTGTACAAGGTGCTGAAACAGGTCCACCCCGA245970.10247571956207202No Hit
AGAACGCAGCTAGCTGCGAGAATTAATGTGAATTGCAGGACACATTGATC245940.102463220998886No Hit
GGAGAGGGCGGCGAAGAGGACCCGCAGGCTGCAAGGAGCAACAGCGATGGTGAGAAGGCAACCAAAGTGCAGGAC240890.10035929619590814No Hit
GAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGCGTTGCTT240730.10029263719224944No Hit
GATGCCTTCCATGGTTTCCTCCCTTCCTTAATTCCCCTATGGTCCTACTT240480.10018848249903271No Hit
AGCTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTC240330.10012598968310267No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)