FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005284529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8039847
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA5966527.421186000181347No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCA2568853.1951478678636547No Hit
TGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTG1550401.9283949060224654No Hit
GGCGACAGGAGGAGAGGCTGTGTGAACAGGAGGAGAGGCTACGTGAACAGGAGGAGAGGCTGTGTGAACAGGAGG947021.1779079875524994No Hit
GTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGT943671.173741241593279No Hit
GGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACC916031.139362477917801No Hit
AATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATG722910.8991589019044766No Hit
CAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAG659900.8207867637282154No Hit
CCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTG642730.7994306359312559No Hit
TATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAA630930.7847537397166886No Hit
TGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAAT574580.7146653412683103No Hit
GCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGG496560.6176236935852137No Hit
CACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCA474100.589687838586978No Hit
CACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGC444030.5522866293351105No Hit
ATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGC425240.528915537820558No Hit
CCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAG415900.5172984013252988No Hit
GAACGAGAGAAGGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCA413960.5148854200832429No Hit
GAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATT398390.4955193799085978No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACAC378160.47035720953396254No Hit
CACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCA369750.45989681146917344No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG356400.4432920178704894No Hit
CCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCAT340840.4239384157434837No Hit
CCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGAGGGTTC316170.3932537522169265No Hit
GGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGT310880.3866740250156502No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG304010.37812908628733854No Hit
CCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACT298740.37157423518134114No Hit
CATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATG296250.3684771613191147No Hit
TGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGAC286420.35625056048952175No Hit
CAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCA274460.34137465551272306No Hit
ACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCA259140.3223195665290645No Hit
ATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTG252970.31464529113551537No Hit
CAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAA243150.30243112835356195No Hit
GTCTTTTTATATTTAACCTAAACCAAATAACACTAATAGAGAAGAACTAATTATAGATGTAAAGTATCTGTAAAT235250.29260507071838554No Hit
ACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTT233400.2903040319050848No Hit
CAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAAGACGAGA228580.2843088929428632No Hit
GAAAGTGATGATGAGGAAGAGGAAGAAAAAGGGCCAGTGTCTCCCAGGAATCTGCAGGAGTCTGAAGAGGAGGA224020.27863714321926775No Hit
AACGAGAGAAGGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCAC223660.278189373504247No Hit
CCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTC213070.26501748105405487No Hit
CTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGT212940.26485578643474184No Hit
ATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGA211310.26282838466950925No Hit
CTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAAAAATCCACTGCCTTCCAA203490.25310183141544856No Hit
GCCCCGACCTAGACGCACTGTAAGTTGTCAGGGTCAATTTAGACACCACT203060.25256699536695165No Hit
AGGAACTCGGCAAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTA199300.24789028945451327No Hit
GTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTTACCA192590.23954435948843303No Hit
CTGGAGGCTTCACAATTCCCTTATAGCCAGCATTCTGGATCCCTACAGTAATGCATTTGCGTGGCTGCAGATGGA192120.23895977124937826No Hit
AATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGAGGGTT191920.23871101029658898No Hit
CTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGG191030.2376040240566767No Hit
GTGAGGGGTGAAGGGGATAAATGCCGGCAGGCTGGACAGGTCGCAAAGAC189750.23601195395882535No Hit
CTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAAGACGAGAAGACCCTATGG185090.2302158237588352No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT183780.22858643951806545No Hit
GGCAAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACC178810.22240472984125192No Hit
GGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCACTGCCGCGTCT177360.22060121293352972No Hit
AGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAA173380.21565086997302313No Hit
CGGCAAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCC172720.2148299588288185No Hit
CGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTA165570.20593675476660192No Hit
CTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGT163760.20368546814385893No Hit
CCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGC161840.2012973629970819No Hit
GTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAAGACG159780.19873512518335237No Hit
GTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAAGACGAGAAGACCCT159140.19793909013442668No Hit
AATTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACT159040.19781470965803205No Hit
TGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTG158270.19685697998979335No Hit
TAATTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTAC157500.1958992503215546No Hit
AGAGAAGGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCACTGCC155470.19337432665074347No Hit
GTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAG151080.18791402373701888No Hit
GGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGA150910.18770257692714798No Hit
AATGGTACAAACTTGCCCACCCGCTAGCCGTTTGGTATGTCTATTAGACT150090.18668265702071196No Hit
CGGGACAAGGGATCAACCTTTCCCGGCAAGGCGGAGGAATTGGGGCAAGA146680.1824412827756548No Hit
AAGAGATGCCTTCCATGGTTTCCTCCCTTCCTTAATTCCCCTATGGTCCT146160.1817945042984027No Hit
CTTCCTTCCTGCCCCGACCTAGACGCACTGTAAGTTGTCAGGGTCAATTT145320.18074970829668774No Hit
AGTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGA143610.17862280215033943No Hit
CTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCT142840.1776650724821007No Hit
CGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGG142640.17741631152931145No Hit
CAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATA142550.17730436910055627No Hit
CATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCT140450.17469237909626886No Hit
TAATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGAGGGT140210.17439386595292175No Hit
CACGTAGTCCCTCAAGGTCTAGGCGGGATAAGAACAAGGATGTACCGTGA136590.16989129270743586No Hit
CGATGAAGAACGCAGCTAGCTGCGAGAATTAATGTGAATTGCAGGACACA136070.16924451423018372No Hit
GGAGAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAA135280.1682619084666661No Hit
GTGTTTGTTTGTTTGTTTGTTTGTTTGTTTGCTTGTTTGTTTGTTTGTTT135200.1681624040855504No Hit
GCAGGTCCTGGTGCTTGATGGTCGAGGCCATCTCCTGGGCCGCCTGGCGG134990.16790120508512163No Hit
GACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT132030.16421954298384037No Hit
GAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTG129490.16106027888341656No Hit
AGGAGAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGA128990.16043837650144338No Hit
GGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGCGTT126740.15763981578256403No Hit
AAAACAGCAGACGGAAAAGTACTGACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCGCTACATATATAGGA126320.15711741778170654No Hit
AGAAAAACGGTGTGCTTCAAATTGCGCTTGTCGGCTATACAAACGCAGGG125000.15547559549329734No Hit
CTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGC122950.15292579572720724No Hit
GGGTTTGTTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTC122700.15261484453622065No Hit
GGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAAGACG122210.15200538020188692No Hit
AGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCAT121320.15089839396197466No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA119280.14836103224352404No Hit
ACTGGCCAGAGGGCTGTGCTGAAGTTTGCTGCTGCCACTGGAGCCACTCCAATTGCTGGCCGCTTCACTCCTGGA118690.14762718743279568No Hit
AGGGTCTCTGAGCATTTCTCAGCCATGTTCAAAAGGAAAGCTTTTGTGCACTGGTACACCAGCGAAGGGATGGAC118040.14681871433623053No Hit
GCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAA116620.14505251157142668No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116000.1442813526177799No Hit
GCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACAC115840.1440823438555485No Hit
CAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTTACC114290.1421544464714316No Hit
GGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAAAAATCCACTGCCTTCCAA111440.13860960289418442No Hit
AGAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGA111100.13818670927444265No Hit
CAGCAATTGAAGTGAAGATGATTGACCGCACGCAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAG108040.13438066669676674No Hit
GAGAAACTGGCAAGGAGAAGCTCCCGCGGTACTACAAGAACATCGGTCTG105910.13173136254956097No Hit
AATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAAGACGAGAAGAC105890.13170648645428204No Hit
GTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCTTGTCGGCTAT105870.1316816103590031No Hit
GGTTTGTTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCT104820.1303756153568594No Hit
CTCTGGCTCTAGAAACAGCAAGAGTCTCCGCACCCCACTTGGAACCATAT104730.13026367292810423No Hit
GTGTGGGCTGGACCAACGGAGGTGTTTAAGATACACAATCTCTGTGAGGT104690.13021392073754637No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT104670.13018904464226744No Hit
CCTGGCTCACGAGGTTGGCTGGAAGTACCAGGCAGTGACAGCCACCCTGGAGGAGAAGAGGAAAGAGAAAGCCA104190.12959201835557319No Hit
ACGAGAGAAGGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCACTGCCGCGTCTGACGGGACAAGGGATC102450.1274277980663065No Hit
GGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCG101550.12630837377875476No Hit
AACAGGAACATCCTCGTGTTAGATATTGAGGCTGCTTCGTGTCGGCACGA101240.12592279430193137No Hit
GCTGCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGCAAGGCGGAGGAATTGGGGCA98170.12210431367661598No Hit
GAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAAC96510.120039597768465No Hit
GTCGCGGGTTCTGCACATATCACGGACGTATCGCTGTCAGACGGACGCTCTCGGACCTAAACCGTAGCTCTCGGC96490.12001472167318607No Hit
GGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA96250.11971620852983893No Hit
TCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCT95530.11882066909979755No Hit
CTTTTCCTCCGCAACCATGTCTGACAAACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGA94300.11729078924014351No Hit
GGCTCAGCTGCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACC93520.11632062152426532No Hit
ATTGGGGCAAGAGGTCCCGGGGAAACGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCATTCATGAAATC92660.11525094942727145No Hit
CACGGACACACCCCCGATGAGTGGTTGCGCTACGGGGCACGATACCATTC91670.11401958271096452No Hit
ATTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTT91420.11370863151997793No Hit
GTGGTTTATGAAATTATTATGTGACCTGTGACTTTCTGATGGCATTTTTG90060.11201705704101085No Hit
GGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAA89580.1114200307543166No Hit
GACAAGGGATCAACCTTTCCCGGCAAGGCGGAGGAATTGGGGCAAGAGGTCCCGGGGAAACGAAACTGGAAACCG89120.11084788056290125No Hit
CTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTT89090.11081056641998287No Hit
AAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTTACCAAAA88880.11054936741955414No Hit
CCCTGGCAGCAGCAGCAGCCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCCACAGCAGCAGCC87590.1089448592740633No Hit
CTGTCATTCGCCATAGAAGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCTTGTCGGCT87360.10865878417835563No Hit
CAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGGATTTGAGGAAAACTGCGACTGTTCTTTAACCAA87240.10850952760668207No Hit
GCAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCA87200.10845977541612423No Hit
TATAGGTTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGATTGTCGCAGCCCGGGTCGATTATAACA87130.10837270908264796No Hit
CGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCTTGTCGGCTA86940.10813638617749816No Hit
ATGGCGTGCCCTCTGGAGAAGGCCCTGGATGTGATGGTGTCCACCTTCCACAAGTACTCGGGCAAAGAGGGTGAC86870.1080493198440219No Hit
CTGAGAGCAAGATGGGTCACCAGCAGCTGTACTGGAGCCACCCGCGAAAA86860.10803688179638245No Hit
CAGTCCTCTGCCAGACCCCTGCCAGACCCCAGTCCACCATGATCCATCTG86780.10793737741526674No Hit
AGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTG85050.10578559517363949No Hit
CTGACAAACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAAAA84770.10543732983973451No Hit
GTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAG84700.10535026350625826No Hit
GTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATA83850.10429302945690386No Hit
GGCGGAGGAATTGGGGCAAGAGGTCCCGGGGAAACGAAACTGGAAACCGA83790.10421840117106707No Hit
GGACCAATGAAGGAACGTAAGGCAAAGGTATGCTCACGAAAAAGAGGTCA83370.10369600317020958No Hit
CCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAAGTAGGGACCTG82020.10201686673888197No Hit
GGCTTCGTTTTGTTTTGCTCTTGAAGGAATTTCCAGTGGTCTAAGGTGCTTTCCTGAGTGGCTCTTTCCA81210.10100938488008541No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG81020.10077306197493559No Hit
GCTGAAGCCAAAGCGAAGGCTTTAAAGGCCAAGAAGGCAGTGTTGAAAGGTGTCCACAGCCACAAAAAGAAGA80960.10069843368909881No Hit
CAGCAGACGGAAAAGTACTGACCAGCGTCACACAAAAACGGAACAGGGCT80950.10068599564145934No Hit
TACTAGGCTAGACTAGCGTACGAGCACTATGGTCAGTAATTCCTGGAGGA80420.10002677911656777No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)