FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005284530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1396568
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC15317710.968101803850583No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAATTCTCGGGTGCCAAGGAACTC749785.368732492796627RNA PCR Primer, Index 1 (100% over 26bp)
CTTTCGAGGCCCTGTAATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA709415.079666725859393RNA PCR Primer, Index 1 (100% over 32bp)
AAATGGATTTTTGGAAATAGGAGATGGAATTCTCGGGTGCCAAGGAACTC641044.59010946835385RNA PCR Primer, Index 1 (100% over 26bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA412182.9513779493730343No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA311102.2276036684214446No Hit
CAAACGAGAACTTTGAAGGCCGAAGTGGAATTCTCGGGTGCCAAGGAACT196011.4035120380819266RNA PCR Primer, Index 1 (100% over 25bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA179951.2885158474202474RNA PCR Primer, Index 1 (100% over 32bp)
TGGTTAGTACTTGGATGGGAGACCGCCTGGAATTCTCGGGTGCCAAGGAA163161.1682925571830374RNA PCR Primer, Index 1 (100% over 23bp)
ATGGATTTTTGGAAATAGGAGATGGAATTCTCGGGTGCCAAGGAACTCCA146731.0506470146817053RNA PCR Primer, Index 1 (100% over 28bp)
AAACGAGAACTTTGAAGGCCGAAGTGGAATTCTCGGGTGCCAAGGAACTC139400.9981612066150735RNA PCR Primer, Index 1 (100% over 26bp)
TCTTTCGAGGCCCTGTAATTGGAATTCTCGGGTGCCAAGGAACTCCAGTC136350.976321954963883RNA PCR Primer, Index 1 (100% over 31bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA98110.7025078621305944No Hit
AACGAGAACTTTGAAGGCCGAAGTGGAATTCTCGGGTGCCAAGGAACTCC93980.6729353672717691RNA PCR Primer, Index 1 (100% over 27bp)
ATGGATTTTTGGAGCAGGGAGATGGAATTCTCGGGTGCCAAGGAACTCCA81600.5842894867990674RNA PCR Primer, Index 1 (100% over 28bp)
AATGGATTTTTGGAAATAGGAGATGGAATTCTCGGGTGCCAAGGAACTCC79870.571901976846097RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA76200.5456232707608939RNA PCR Primer, Index 1 (100% over 28bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA71550.5123273625057999No Hit
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC71200.509821218873696RNA PCR Primer, Index 1 (100% over 27bp)
GAGGCCCTGTAATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGTA63480.4545428507598628RNA PCR Primer, Index 22 (97% over 37bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG56370.4036323329762675RNA PCR Primer, Index 6 (100% over 34bp)
GGCTGGTCCGATGGTAGTGGGTTATCTGGAATTCTCGGGTGCCAAGGAAC53800.3852300783062479RNA PCR Primer, Index 1 (100% over 24bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG50700.36303280613618527Illumina Small RNA Adapter 2 (100% over 21bp)
AATGGATTTTTGGAGCAGGGAGATGGAATTCTCGGGTGCCAAGGAACTCC49160.3520057741549284RNA PCR Primer, Index 1 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA48670.348497173069983No Hit
TTTCGAGGCCCTGTAATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC48460.3469934868907207RNA PCR Primer, Index 1 (100% over 33bp)
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC40570.2904978490127226No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA39530.28305102222018547No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC37240.2666536824558489RNA PCR Primer, Index 1 (100% over 27bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36690.26271545674825714No Hit
GTTTCCGTAGTGTAGTGGTTATCACTTTCGCCTTGGAATTCTCGGGTGCC35690.2555550463708176No Hit
GATATAGACAGCAGGACGGTGGCCATGGAATTCTCGGGTGCCAAGGAACT34320.2457452841537254RNA PCR Primer, Index 1 (100% over 25bp)
GAGAACTTTGAAGGCCGAAGTGGAATTCTCGGGTGCCAAGGAACTCCAGT33330.23865647788006025RNA PCR Primer, Index 1 (100% over 30bp)
CCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAACT30030.21502712363450976RNA PCR Primer, Index 1 (100% over 25bp)
CAACTCTTAACAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGTA29190.20901237891746052RNA PCR Primer, Index 22 (97% over 37bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG27380.19605203613429495No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA27220.1949063704739046No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATTCTCGGGTGCCAAGG25690.18395094259642208Illumina Small RNA Adapter 2 (100% over 21bp)
ACGAGAACTTTGAAGGCCGAAGTGGAATTCTCGGGTGCCAAGGAACTCCA25120.17986950868128154RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA24850.17793619787937287No Hit
AGAACTTTGAAGGCCGAAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC19530.13984281467139445RNA PCR Primer, Index 1 (100% over 31bp)
CACGGACCAAGGAGTCTAACATATCTGGAATTCTCGGGTGCCAAGGAACT19080.13662063000154664RNA PCR Primer, Index 1 (100% over 25bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC18490.1323959878788573No Hit
CTGAGAAGACGGTCGAACTTGACTATCTAGTGGAATTCTCGGGTGCCAAG18300.1310355099071438No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA17710.12681086778445447RNA PCR Primer, Index 1 (100% over 23bp)
CTGGACGCGAGCCGGGCCCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG17010.12179858052024678RNA PCR Primer, Index 1 (100% over 29bp)
AGTACTTGGATGGGAGACCGCCTGGAATTCTCGGGTGCCAAGGAACTCCA16420.11757393839755745RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC16410.11750233429378305No Hit
GTAGCAATATCTGTTTAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA16230.11621346042584393RNA PCR Primer, Index 1 (100% over 32bp)
GGACTCTTTCGAGGCCCTGTAATTGGAATTCTCGGGTGCCAAGGAACTCC15880.11370731679374009RNA PCR Primer, Index 1 (100% over 27bp)
CTGAGAAGACGGTCGAACTTGACTTGGAATTCTCGGGTGCCAAGGAACTC15840.1134209003786425RNA PCR Primer, Index 1 (100% over 26bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGGAATTCTCGGGTGCCAAGGA15670.11220363061447777RNA PCR Primer, Index 1 (100% over 22bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTATCGGGTGCC15360.10998390339747152No Hit
ATTAAATGGATTTTTGGAAATAGGAGATGGAATTCTCGGGTGCCAAGGAA15120.10826540490688602RNA PCR Primer, Index 1 (100% over 23bp)
TTCGAGGCCCTGTAATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG14930.1069049269351725RNA PCR Primer, Index 6 (100% over 34bp)
GTTTCCGTAGTGTAGTGGTTATCACATTCGCCTTGGAATTCTCGGGTGCC14730.10547284485968461No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG14080.10081857811434888No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)