FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005284533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2739439
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA56184220.50938166537017RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC1924717.025927571302007RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG933163.4063908705395525RNA PCR Primer, Index 1 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA472961.7264848751879491No Hit
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC451091.6466510113932087RNA PCR Primer, Index 1 (100% over 27bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA407511.487567344992898RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC402891.4707025781556005RNA PCR Primer, Index 1 (100% over 27bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA230590.8417416850676361RNA PCR Primer, Index 1 (100% over 32bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA178510.6516297679926437RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTGCCAAGGAACTCC106260.3878896372578473RNA PCR Primer, Index 1 (100% over 27bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA98780.36058477666412725RNA PCR Primer, Index 1 (100% over 34bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG89690.32740280035437913Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT85490.3120711941386539RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGGGCAGAGAGCGAGACTTGGAATTCTCGGGTGCCAAGGAACTCCAG81760.298455267666117RNA PCR Primer, Index 1 (100% over 29bp)
CAAACGAGAACTTTGAAGGCCGAAGTGGAATTCTCGGGTGCCAAGGAACT81260.29663007644995926RNA PCR Primer, Index 1 (100% over 25bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA72600.2650177645861069RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA70810.2584835800322621No Hit
AAACGAGAACTTTGAAGGCCGAAGTGGAATTCTCGGGTGCCAAGGAACTC66490.24271392792465904RNA PCR Primer, Index 1 (100% over 26bp)
AACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA66370.24227588203278114RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA53560.1955144830748193RNA PCR Primer, Index 1 (100% over 28bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG51790.18905330616962088No Hit
CTTTCGAGGCCCTGTAATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA44760.16339111767044273RNA PCR Primer, Index 1 (100% over 32bp)
TGGTTAGTACTTGGATGGGAGACCGCCTGGAATTCTCGGGTGCCAAGGAA44460.16229600294074809RNA PCR Primer, Index 1 (100% over 23bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA43140.1574774981300916RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT43120.15740449048144528RNA PCR Primer, Index 1 (100% over 30bp)
TCCTGTACTGAGCTGCCCCGAGATTGGAATTCTCGGGTGCCAAGGAACTC43000.15696644458956743RNA PCR Primer, Index 1 (100% over 26bp)
TCTTAACCCGGACCAGAAACTATGGAATTCTCGGGTGCCAAGGAACTCCA42480.15506824572476335RNA PCR Primer, Index 1 (100% over 28bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA41040.149811695022229RNA PCR Primer, Index 1 (100% over 28bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA39930.14575977052235878RNA PCR Primer, Index 1 (100% over 34bp)
TCCTGTACTGAGCTGCCCCGAGAATGGAATTCTCGGGTGCCAAGGAACTC34900.1273983468878117RNA PCR Primer, Index 1 (100% over 26bp)
CAACTCTTAACAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTT32040.1169582531313893RNA PCR Primer, Index 8 (97% over 37bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA31910.1164837034151883RNA PCR Primer, Index 1 (100% over 28bp)
GTTGAATTGTGGTCTGTGGACGTGGAATTCTCGGGTGCCAAGGAACTCCA31620.11542509250981679RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG30890.11276031333422645No Hit
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT30570.11159219095588549RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGGGCAGAGAGCGAGACTTTGGAATTCTCGGGTGCCAAGGAACTCCA30180.11016854180728244RNA PCR Primer, Index 1 (100% over 28bp)
CTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGT28730.10487548728042492RNA PCR Primer, Index 1 (100% over 30bp)
AGGTTCCGGATAAGTAAGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCA27790.10144412779404834RNA PCR Primer, Index 1 (100% over 28bp)
ACGAGAACTTTGAAGGCCGAAGTGGAATTCTCGGGTGCCAAGGAACTCCA27500.10038551688867683RNA PCR Primer, Index 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACTCC150.002223508270.0136953
GCTAGCC150.002223508270.0136956
CATGTTA150.002223508270.0136953
ATAGCCG207.923607E-570.012421
CTAGGCG207.923607E-570.012421
CCCCACC150.002224796570.003467
GCCTTAC207.9300575E-570.00091614
CCGATAC207.9300575E-570.0009169
CGCGCTA252.8418235E-670.00091614
AGCCCAC150.002225118770.0009114
TGTTACC207.930774E-569.9996412
GCGCTAC252.842131E-669.9996415
TTCAGCG150.002225279669.9996312
AGCTTCC2550.069.9996311
GGTCCAC150.002225279669.9996312
CCCACCC150.002225279669.9996319
TACGCAA150.002225279669.9996315
CCCCCTA150.002225279669.9996315
TTGCCCC1250.069.9996313
CTAGCGC150.002225279669.9996320
Produced by FastQC (version 0.11.5)